A5016349252- NF-κB Signaling Pathways
- Muscle metabolism and nutrition
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Ubiquitin and proteasome pathways
- Cholesterol and Lipid Metabolism
- Immune Cell Function and Interaction
- Inflammatory mediators and NSAID effects
- Alkaline Phosphatase Research Studies
- Pancreatic function and diabetes
- interferon and immune responses
- Diet, Metabolism, and Disease
- Drug-Induced Hepatotoxicity and Protection
- Protein Kinase Regulation and GTPase Signaling
- Phagocytosis and Immune Regulation
- Immune cells in cancer
- Metabolism, Diabetes, and Cancer
- Nuclear Receptors and Signaling
- Erythrocyte Function and Pathophysiology
- Phytase and its Applications
- Cancer-related Molecular Pathways
- Mitochondrial Function and Pathology
Instituto de Investigaciones Biomédicas Sols-Morreale
2014-2024
Universidad Autónoma de Madrid
2014-2024
Consejo Superior de Investigaciones Científicas
2014-2024
Universidad de Las Palmas de Gran Canaria
2016-2023
Instituto de Biomedicina y Biotecnología de Cantabria
2016
Indian Institute of Business Management Patna
2010
Weatherford College
1993
Hospital Universitario Fundación Jiménez Díaz
1980-1990
University of Dundee
1984-1987
We have measured the activity of S -adenosyl-L-methionine synthetase, ratio between high- and low-molecular-weight forms this enzyme concentration in liver biopsies from a group controls (n = 6) six cirrhotics (five posthepatitic one alcoholic). The total synthetase was markedly reduced cirrhosis (37.5% that found control group). This due to specific reduction high-molecular-weight (73.9 pmoles per min mg protein) when compared with observed (460.3 protein). Despite rate synthesis (the form...
Protein phosphatases‐2A o , 2A 1 and 2 have been purified to homogeneity from rabbit skeletal muscle. Approximately mg of phosphatase‐2A 0 0.5 was isolated 4000 g muscle within 10 days. each comprised three subunits, termed A, B' C (2A ) or B ), while contained only two A C. The components had indistinguishable mobilities on sodium dodecyl sulphate/ polyacrylamide gels identical peptide maps. By these criteria, the component also catalytic subunit ethanol‐treated extracts. electrophoretic B′...
Sirtuin 1 (SIRT1) is a key player in liver physiology and therapeutic target against hepatic inflammation. We evaluated the role of SIRT1 proinflammatory context oxidative stress during acetaminophen (APAP)-mediated hepatotoxicity.SIRT1 protein levels decreased human mouse livers following APAP overdose. SIRT1-Tg mice maintained higher on injection than wild-type were protected hepatotoxicity by modulation antioxidant systems restrained inflammatory responses, with stress, cytokine messenger...
Only two S-adenosyl-L-methionine synthetase forms exist in rat liver: high-Mr and low-Mr synthetase, which have been purified to apparent homogeneity as judged by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. High-Mr had an molecular mass, determined filtration, of 210 kDa was a tetramer constituted 48.5-kDa subunits, estimated The mass 110 subunits 47 kDa. An antiserum against cross-reacted with the forms. Reverse-phase HPLC runs tryptic digestions showed that peptide maps were...
The dephosphorylation of glycogen synthase by protein phosphatase‐1 in hepatic and microsomes was inhibited nanomolar concentrations phosphorylase a . I 50 for 1000‐fold lower than its K m as substrate, while tryptic digestion increased the without affecting Protein from skeletal muscle phosphatase‐2A liver were only at higher concentrations. became desensitized to when released microsomes, but sensititvity partially restored readdition solubilized enzyme microsomes. results demonstrate that...
A 2.5 kb clone containing the full‐length coding sequence of a type‐2A protein phosphatase catalytic subunit has been isolated from rabbit skeletal muscle cDNA library constructed in λgt10. The deduced contains 309 residues (35.58 kDa). major mRNA species at 2.0 and minor component 2.8 were visualized by Northern blotting both liver. enzyme showed weak homology with mammalian alkaline phosphatases between 55 95. differs that reported for bovine adrenal three regions.
Glucagon produces a time- and dose-dependent activation of phospholipid methyltransferase activity in isolated rat hepatocytes. Half-maximal effect is caused by dose glucagon 1 x 10(-10) M. This due to an increase the Vmax value enzyme, without affecting Km for S-adenosylmethionine. Exogenous cyclic AMP added hepatocytes mimics glucagon, nonsaturating concentration spontaneously reversible within 40 min incubation.
LPS stimulation activates IKK and different MAP kinase pathways, as well the PI3K-Akt-mTOR-p70 S6k pathway, a negative regulator of these MyD88-dependent intracellular signals. Here, we show that Cot/tpl2, MAP3K responsible for activation MKK1-Erk1/2, controls P-Ser473 Akt P-Thr389 p70 phosphorylation in LPS-stimulated macrophages. Analysis signalling Cot/tpl2 KO macrophages versus WT reveals lower IκBα recovery higher JNK p38α after 1 h stimulation. Moreover, deficiency increases...
Macrophages are present in a large variety of locations, playing distinct functions that determined by its developmental origin and the nature activators microenvironment. Macrophage activation can be classified as pro-inflammatory (M1 polarization) or anti-inflammatory-pro-resolution-deactivation (M2), these profiles coexisting course immune response relevant functional role onset inflammation (Figure 1). Several groups have analysed metabolic aspects associated with macrophage to answer...
Glycogen synthase (labelled in sites-3) and glycogen phosphorylase from rabbit skeletal muscle were used as substrates to investigate the nature of protein phosphatases that act on these proteins microsomal fractions rat liver. Under assay conditions employed, phosphatase activities both subcellular could be inhibited 80–90% by inhibitor-1 or inhibitor-2, concentrations required for half-maximal inhibition similar. coeluted Sephadex G-100 broad peaks, stretching void volume an apparent...
The liver X receptors α and β (LXRα LXRβ) are oxysterol-activated transcription factors that coordinately regulate gene expression is important for cholesterol fatty acid metabolism. In addition to their roles in lipid metabolism, LXRs participate the transcriptional regulation of macrophage activation considered potent regulators inflammation. highly similar, despite notable exceptions, most reported functions substantially overlapping. However, individual genomic distribution capacities...
Cyclooxygenase-2 (COX-2) is induced in human T lymphocytes upon cell receptor triggering. Here we report that Cot kinase, a mitogen-activated protein kinase involved activation, up-regulates COX-2 gene expression Jurkat cells. Induction of promoter activity by occurred mainly through activation the nuclear factor activated cells (NFAT). Mutation distal (−105/−97) and proximal (−76/−61) NFAT response elements abolished kinase. Even more, coexpression dominant negative version inhibited...
Antibody prepared against the catalytic subunit of protein phosphatase-2A from rabbit skeletal muscle, could completely inhibit this enzyme, but did not significantly affect activities phosphatases-1, 2B and 2C. The antibody was used to establish following points. 1 three forms that can be resolved by ion-exchange chromatography, termed 2A0, 2A1, 2A2, share same subunit. 2 antigenic sites on remain accessible antibody, when is complexed with other subunits phosphatases-2A0,2A1 2A2. 3...
Cot/tpl2 is the only MAP3K that activates MKK1/2-Erk1/2 in Toll-like receptor-activated macrophages. Here we show regulates RSK, S6 ribosomal protein, and 4E-BP phosphorylation after stimulation of bone marrow-derived macrophages with lipopolysaccharide (LPS), poly I:C, or zymosan. The dissociation 4E-BP-eIF4E complex, a key event cap-dependent mRNA translation initiation, dramatically reduced LPS-stimulated Cot/tpl2-knockout (KO) versus wild-type (Wt) Accordingly, LPS activation, increased...
NGFI-A is an immediate early gene, encoding a zinc finger protein, rapidly activated after mitogenic stimulation.NGFI-A gene expression was found to be and transiently induced interleukin-2 (IL-2) stimulation of GI lymphoblasts, as well during the Go/G1 transition, when stimulated with concanavalin A (ConA).Activation both Ca2+ protein kinase C pathways, separately, in quiescent T lymphocytes produced partial induction this gene; however, stimuli together are necessary obtain full...
The methods to obtain chitooligosaccharides are tightly related the physicochemical properties of end products. Knowledge these characteristics is crucial describing biological functions chitooligosaccharides. Chitooligosaccharides were prepared either in a single-step enzymatic hydrolysis using chitosanase, or two-step chemical-enzymatic hydrolysis. hydrolyzed products obtained preparation composed mainly 42% fully deacetylated oligomers plus 54% monoacetylated oligomers, and they...
Research Article16 April 2018Open Access Source DataTransparent process Myeloid cell deficiency of p38γ/p38δ protects against candidiasis and regulates antifungal immunity Dayanira Alsina-Beauchamp Department Immunology Oncology, Centro Nacional de Biotecnología/CSIC, Madrid, Spain Search for more papers by this author Alejandra Escós orcid.org/0000-0002-2990-7920 Pilar Fajardo Diego González-Romero Ester Díaz-Mora Ana Risco Miguel A Martín-Serrano Carlos del Fresno Immunobiology...
Cot, initially identified as an oncogene in a truncated form, is mitogen-activated protein kinase implicated cellular activation and proliferation. Here, we show that this truncation of Cot results 10-fold increase its overall activity through two different mechanisms. Truncated exhibits lower turnover rate (half-life, 95 min) than wild-type 35 min). The degradation can be specifically inhibited by proteasome inhibitors situ. 20S also degrades more efficiently the protein. Furthermore, amino...