This study was conducted to investigate the modulating effects of green tea polyphenols on genotoxic damage and apoptotic activity induced by hexavalent chromium [Cr (VI)] in CD-1 mice. Animals were divided into following groups: (i) injected with vehicle; (ii) treated (30 mg/kg) via gavage; (iii) CrO 3 (20 intraperitoneally; (iv) addition . Genotoxic evaluated examining micronucleated polychromatic erythrocytes (MN-PCEs) obtained from peripheral blood at 0, 24, 48, 72 h after treatment....

The chemotactic activity and random motility of neutrophils, was studied in 38 patients with hypovitaminosis D rickets, compared 29 healthy controls matched age. derived from the activated rickets serum as well amounts complement components C4, C3 C5 normal, but cell clearly defective (p less than 0.001). A possible relationship between neutrophil movement recurrent infections seen these is suggested. mechanisms responsible for defect could be alteration Ca/P metabolism or a action vitamin...

This study aimed to examine the dose-response effects of polyphenon-60 derived from green tea (P60-GT) on hexavalent chromium [Cr(VI)]-induced genotoxic damage and apoptosis. Male Hsd:ICR mice were divided into 4 groups: (1) Control (vehicle only), (2) P60-GT (15, 30, or 45 mg/kg gavage), (3) Cr(VI) (20 CrO3 intraperitoneally), (4) P60-GT+CrO3 (P60-GT administered hr before CrO3). Peripheral blood samples collected at 24, 48, 72 assess number micronuclei (MN), apoptosis, cell viability,...

Abstract This study was conducted to investigate the modulating effects of (–)-epigallocatechin-3-gallate (EGCG), quercetin, and rutin on genotoxic damage induced by Cr(VI) in polychromatic erythrocytes CD-1 mice. The animals were divided into following groups: (i) vehicle only; (ii) flavonoids (10 mg/kg EGCG, 100 625 rutin, or 100–625 quercetin–rutin); (iii) (20 CrO3); (iv) concomitantly with Cr(VI). All treatments administered intraperitoneally (ip). evaluated based number micronucleated...

Abstract Most cases of CVID occur sporadically, but familial do also and 15% the patients with disease have first degree relatives IgA deficiency (IgAD). Our purpose was to study association HLA class II alleles ascertain whether this shares a common genetic background IgAD in our population. Patients (n = 42), were typed using gene amplification sequence-specific oligonucleotide probing for HLA-DRB1, DRB3, DQA1 DQB1 loci their typing compared that 96 334 healthy controls. We observed...

This study was conducted to investigate the effects of vanadium pentoxide (V 2 O 5 ), ascorbic acid (AA), and alpha‐tocopherol ( α ‐TOH) on apoptotic, cytotoxic, genotoxic activity. Groups five Hsd:ICR mice were treated with following: (a) vehicle, distilled water; (b) corn oil; (c) AA, 100 mg/kg intraperitoneally (ip); (d) ‐TOH, 20 by gavage; (e) V , 40 ip injection; (f) AA + ; (g) ‐TOH . Genotoxic damage evaluated examining micronucleated polychromatic erythrocytes (MN‐PCE) obtained from...

This study aimed to investigate the relationship between endogenous antioxidant system, 8-hydroxydeoxyguanosine adduct (8-OHdG) repair, and apoptosis in mice treated with chromium(VI) alone presence of antigenotoxic compound (-)-epigallocatechin-3-gallate (EGCG). Groups 5 Hsd:ICR male were divided as follows: (1) control, vehicle only; (2) EGCG, 8.5 mg/kg by gavage alone; (3) CrO3, 20 intraperitoneally (4) EGCG combined was administered 4 hr prior CrO3. Peripheral blood parameters analyzed...

This study was conducted to investigate the relationship between modulation of genotoxic damage and apoptotic activity in Hsd:ICR male mice treated with (–)-epigallocatechin-3-gallate (EGCG) hexavalent chromium [Cr(VI)]. Four groups 5 each were (i) control vehicle only, (ii) EGCG (10 mg/kg) by gavage, (iii) Cr(VI) (20 mg/kg CrO3) intraperitoneally (ip), (iv) addition CrO3 (EGCG-CrO3). Genotoxic evaluated examining presence micronucleated polychromatic erythrocytes (MN-PCE) obtained from...

Abstract Mutations in the TNFRSF13B ( TACI ) gene have been associated with common variable immunodeficiency, and a role immunoglobulin A deficiency (IgAD) has also suggested. We aimed at studying of several polymorphisms along this IgAD susceptibility. Three mutations (C104R, A181E R202H) eight additional single nucleotide were genotyped 338 Spanish patients 553 ethnically matched healthy controls tested for association. Data from parents 114 collected used analysis. No statistically...

SUMMARY X-linked hyper-IgM syndrome (HIGM1) (MIM♯308230), is a severe primary immunodeficiency caused by mutations in the gene coding for CD40 ligand (CD40L or CD154), member of tumour necrosis factor (TNF) superfamily. The interaction this protein with its ligand, CD40, mediates crucial processes immune response. variety defects that have been described HIGM1 patients range from complete lack CD40L expression to missense interfere CD40L. In study we describe three families – total seven and...

Exposure to chromium (VI) [Cr(VI)] has been postulated be associated with the induction of cancer. In vivo studies utilizing biomarkers genotoxic damage could aid in elucidating mechanisms underlying effects Cr(VI) and their relationship carcinogenesis. this study, origin (clastogenic and/or aneugenic damage) kinetics micronuclei (MN) induced by were investigated.Hsd:ICR female mice divided into groups five individuals each. MN measured treated 20 or 25 mg/kg CrO3 intraperitoneally using...

Consumption of green tea, by its antioxidant properties, has been associated with beneficial health effects, because may play a role in the risk and pathogenesis several chronic diseases, especially cardiovascular disease cancer. On other hand, it reported that metal compounds such as chromium [VI] are carcinogenic can induce genotoxic damage through Oxidative Stress. Therefore, is possible tea protective effect against induced this compounds.To evaluate oral administration over Cr...

In vitro assays have demonstrated that vanadium compounds interact with biological molecules similar to protein kinases and phosphatases also shown oxides decrease the proliferation of cells, including human lymphocytes; however, mechanism, phase in which cell cycle is delayed proteins involved this process are unknown. Therefore, we evaluated effects (V2 O3 , V2 O4 O5 ) lymphocyte cultures (concentrations 2, 4, 8, or 16 μg/ml) on cellular levels p53, p21 Cdc25C proteins. After 24 h...

Summary Common variable immunodeficiency (CVID) is a very frequent but heterogeneous syndrome of antibody formation. The primary defect remains unknown, many reports describe peripheral blood T lymphocyte dysfunctions in substantial proportion CVID patients, which may impair T–B cell collaboration. In order to investigate whether such putative defects were intrinsic cells or, rather, secondary quantitative differences subset distribution, or other described disorders, we have used...