A5051538398- Immune Response and Inflammation
- Immune cells in cancer
- Immune responses and vaccinations
- Immunotherapy and Immune Responses
- Inflammation biomarkers and pathways
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Antifungal resistance and susceptibility
- Vaccine Coverage and Hesitancy
- Cystic Fibrosis Research Advances
- Fungal Infections and Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- Lung Cancer Research Studies
- COVID-19 Impact on Reproduction
- Bacterial Identification and Susceptibility Testing
- Phagocytosis and Immune Regulation
- Gut microbiota and health
- NF-κB Signaling Pathways
- Neutropenia and Cancer Infections
Hospital Universitario La Paz
2004-2025
Hospital La Paz Institute for Health Research
2011-2024
Spanish National Centre for Cardiovascular Research
2013-2022
Hospital General Universitario Gregorio Marañón
2014-2020
Universidad Complutense de Madrid
2017
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2017
Instituto de Salud Carlos III
2013
Centro Nacional de Biotecnología
2012-2013
Consejo Superior de Investigaciones Científicas
2012-2013
Universidad Autónoma de Madrid
2012
Neutrophils require directional cues to navigate through the complex structure of venular walls and into inflamed tissues. Here we applied confocal intravital microscopy analyze neutrophil emigration in cytokine-stimulated mouse cremaster muscles. We identified differential non-redundant roles for chemokines CXCL1 CXCL2, governed by their distinct cellular sources. was produced mainly TNF-stimulated endothelial cells (ECs) pericytes supported luminal sub-EC crawling. Conversely, neutrophils...
Monocyte exposure to LPS induces a transient state in which these cells are refractory further endotoxin stimulation. This phenomenon, termed tolerance (ET), is characterized by decreased production of cytokines response the proinflammatory stimulus. We have established robust model ET and determined time frame features unresponsiveness cultured human monocytes. A large number genes transcribed tolerant monocytes were classified as either "tolerizable" or "nontolerizable" depending on their...
Abstract Triggering receptors expressed on myeloid cell (TREM) proteins are a family of surface that participate in diverse cellular processes such as inflammation, coagulation, and bone homeostasis. TREM-1, particular, is neutrophils monocytes potent amplifier inflammatory responses. LPS other microbial products induce up-regulation surface-localized TREM-1 the release its soluble form, sTREM-1. Two hypotheses have been advanced to explain origin sTREM-1: alternative splicing mRNA...
On the basis mainly of pharmacological experiments, p38α MAP kinase isoform has been established as an important regulator immune and inflammatory responses. However, role related p38γ p38δ kinases remained unclear. Here, we show that deletion impaired innate response to lipopolysaccharide (LPS), a Toll-like receptor 4 (TLR4) ligand, by blocking extracellular signal-regulated 1/2 (ERK1/2) activation in macrophages dendritic cells. were necessary maintain steady-state levels tumor progression...
The continuous dissemination of the novel emerging SARS-CoV-2 virus, with more than 78 million infected cases worldwide and higher 1,700,000 deaths as 23 December 2020, highlights urgent need for development vaccines against COVID-19. With this aim, we have developed vaccine candidates based on poxvirus modified vaccinia virus Ankara (MVA) strain expressing full-length spike (S) protein, evaluated their immunogenicity in mice using DNA/MVA or MVA/MVA prime/boost immunization protocols.
Host injury triggers feedback mechanisms that limit tissue damage. Conventional type 1 dendritic cells (cDC1s) express cell natural killer lectin group receptor-1 (DNGR-1), encoded by the gene Clec9a, which senses damage and favors cross-presentation of dead-cell material to CD8+ T cells. Here we find DNGR-1 additionally reduces host-damaging inflammatory responses induced sterile infectious in mice. deficiency leads exacerbated caerulein-induced necrotizing pancreatitis increased pathology...
MV130 is an inactivated polybacterial mucosal vaccine that confers protection to patients against recurrent respiratory infections, including those of viral etiology. However, its mechanism action remains poorly understood. Here, we find intranasal prophylaxis with modulates the lung immune landscape and provides long-term heterologous infections in mice. Intranasal administration systemic candidiasis wild-type Rag1-deficient mice lacking functional lymphocytes, indicative innate...
Sepsis is associated with T-cell exhaustion, which significantly reduces patient outcomes. Therefore, targeting of immune checkpoints (ICs) deemed necessary for effective sepsis management. Here, we evaluated the role SIGLEC5 as an IC ligand and explored its potential a biomarker sepsis.In vitro in vivo assays were conducted to both analyse SIGLEC5's ligand, well assess impact on survival sepsis. A multicentre prospective cohort study was evaluate plasmatic soluble (sSIGLEC5) mortality...
Impairment of the intestinal barrier allows systemic translocation commensal bacteria, inducing a proinflammatory state in host. Here, we investigated innate immune responses following increased gut permeability upon administration dextran sulfate sodium (DSS) mice. We found that Enterococcus faecalis translocated to bone marrow DSS treatment and induced trained immunity (TI) hallmarks bone-marrow-derived mouse macrophages human monocytes. or heat-killed E. reprogrammed progenitors (BMPs),...
Abstract Although blood monocytes possess significant cytotoxic activity against tumor cells, tumor-infiltrating are commonly deactivated in cancer patients. Monocytes pre-exposed to cells show significantly decreased expression levels of TNF-α, IL-12p40, and IL-1R-associated kinase (IRAK)-1. Activation the Ser/Thr IRAK-1 is an important event several inflammatory processes. By contrast, another IRAK family member, IRAK-M, negatively regulates this pathway, up-regulated cultures...
Abstract Repeated exposure to low doses of endotoxin results in progressive hyporesponsiveness subsequent challenge, a phenomenon known as tolerance. In spite its clinical significance sepsis and characterization the TLR4 signaling pathway principal detection mechanism, molecular determinants that induce tolerance remain obscure. We investigated role TRIF/IFN-β TLR4-induced Lipid A-induced homotolerance was characterized by down-regulation MyD88-dependent proinflammatory cytokines TNF-α...
β-Glucan-induced trained immunity in myeloid cells leads to long-term protection against secondary infections. Although previous studies have characterized this phenomenon, strategies boost remain undefined. We found that β-glucan-trained macrophages from mice with a myeloid-specific deletion of the phosphatase SHIP-1 (LysMΔSHIP-1) showed enhanced proinflammatory cytokine production response lipopolysaccharide. Following β-glucan training, SHIP-1-deficient exhibited increased phosphorylation...
The molecular mechanisms through which dendritic cells (DCs) prime T helper 2 (Th2) responses, including those elicited by parasitic helminths, remain incompletely understood. Here, we report that soluble egg antigen (SEA) from Schistosoma mansoni, is well known to drive potent Th2 triggers DCs produce prostaglandin E2 (PGE2), subsequently—in an autocrine manner—induces OX40 ligand (OX40L) expression license these responses. Mechanistically, SEA was found promote PGE2 synthesis Dectin-1 and...
Brain CD11c+ cells share features with microglia and dendritic (DCs). Sterile inflammation increases brain cells, but their phenotype, origin, functions remain largely unknown. We report that, after cerebral ischemia, attract DCs to the inflamed brain, astroglia produce Flt3 ligand, supporting development expansion of cells. in are a complex population derived from proliferating infiltrating DCs, including major subset OX40L+ conventional cDC2, also cDC1, plasmacytoid, monocyte-derived DCs....
We have analyzed BNT162b2 vaccine-induced immune responses in naive subjects and individuals recovered from coronavirus disease 2019 (COVID-19), both soon after (14 days) later (almost 8 months) vaccination. Plasma spike (S)-specific immunoglobulins peak one vaccine shot COVID-19, while a second dose is needed subjects, although the latter group shows reduced levels all along period. Despite how neutralization capacity against severe acute respiratory syndrome 2 (SARS-CoV-2) mirrors this...
Intravesical administration of Bacillus Calmette-Guérin (BCG) was one the first FDA-approved immunotherapies and remains a standard treatment for bladder cancer. Previous studies have demonstrated that intravenous (IV) BCG is well-tolerated effective in preventing tuberculosis infection animals. Here, we examine IV several preclinical lung tumor models. Our findings demonstrate inoculation reduced growth prolonged mouse survival models melanoma metastasis orthotopic adenocarcinoma. Moreover,...