A5015162064- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Optical Imaging and Spectroscopy Techniques
- Neurological Disease Mechanisms and Treatments
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Advanced Fluorescence Microscopy Techniques
- Atherosclerosis and Cardiovascular Diseases
- Phagocytosis and Immune Regulation
- Chemokine receptors and signaling
- Barrier Structure and Function Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Reproductive System and Pregnancy
- Cell Image Analysis Techniques
- dental development and anomalies
- Immune Response and Inflammation
- Photoacoustic and Ultrasonic Imaging
- Platelet Disorders and Treatments
- Inflammatory mediators and NSAID effects
- Oral and Maxillofacial Pathology
- Bone and Dental Protein Studies
- S100 Proteins and Annexins
- Eicosanoids and Hypertension Pharmacology
- Neurogenesis and neuroplasticity mechanisms
Achucarro Basque Center for Neuroscience
2022-2023
University of the Basque Country
2022-2023
Institut Interdisciplinaire de Neuroscience
2022-2023
Centre Interdisciplinaire de Recherche en Biologie
2023
Université de Bordeaux
2022
Centre National de la Recherche Scientifique
2022
Institut d'Investigacions Biomèdiques de Barcelona
2017-2020
Consejo Superior de Investigaciones Científicas
2017-2020
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2019-2020
Universitat Autònoma de Barcelona
2015
Stroke attracts neutrophils to the injured brain tissue where they can damage integrity of blood-brain barrier and exacerbate lesion. However, mechanisms involved in neutrophil transmigration, location accumulation ischemic are not fully elucidated. Neutrophils reach perivascular spaces vessels after crossing endothelial cell layer basal lamina post-capillary venules, or migrating from leptomeninges following pial vessel extravasation and/or a suggested translocation skull bone marrow. Based...
Background and Purpose- Ischemia attracts neutrophils to the injured brain. However, neutrophil location access damaged brain tissue is not yet entirely understood. We aimed investigate in a mouse model of cerebral ischemia/reperfusion. Methods- Adult male C57BL/6 mice (n=52) received 45-minute intraluminal middle artery occlusion followed by 14, 24, 48, or 96 hours reperfusion. Sham-operated (n=9) were subjected entire surgical procedure. used wild-type CatchupIVM expressing red fluorescent...
Inflammatory Ly6C hi CCR2 + monocytes infiltrate the brain after stroke but their functions are not entirely clear. We report that and lymphocytes permanent ischemia. To underscore role of monocytes, we generated mice with selective deletion in monocytes. One day post-ischemia, these showed less infiltrating reduced expression pro-inflammatory cytokines, markers alternatively macrophage activation, angiogenesis. Accordingly, sorted from wild type 24 h post-ischemia expressed genes, M2...
Brain CD11c+ cells share features with microglia and dendritic (DCs). Sterile inflammation increases brain cells, but their phenotype, origin, functions remain largely unknown. We report that, after cerebral ischemia, attract DCs to the inflamed brain, astroglia produce Flt3 ligand, supporting development expansion of cells. in are a complex population derived from proliferating infiltrating DCs, including major subset OX40L+ conventional cDC2, also cDC1, plasmacytoid, monocyte-derived DCs....
Microglia are very sensitive to changes in the environment and respond through morphological, functional metabolic adaptations. To depict modifications microglia undergo under healthy pathological conditions, we developed free access image analysis scripts quantify morphologies phagocytosis. Neuron-glia cultures, which express reporter tdTomato, were exposed excitotoxicity or + inflammation analysed 8 h later. Neuronal death was assessed by SYTOX staining of nucleus debris phagocytosis...
Abnormalities in myelination are associated to behavioral and cognitive dysfunction neurodevelopmental psychiatric disorders. Thus, therapies promote or accelerate could potentially ameliorate symptoms autism. Clemastine, a histamine H1 antagonist with anticholinergic properties against muscarinic M1 receptor, is the most promising drug promyelinating properties. Clemastine penetrates blood brain barrier efficiently promotes remyelination different animal models of neurodegeneration...
Cyclooxygenase-2 (COX-2) is induced under inflammatory conditions, and prostaglandin E2 (PGE2) one of the products COX activity. PGE2 has pleiotropic actions depending on activation specific E-type prostanoid EP1-4 receptors. We investigated involvement EP receptors in glial response to an challenge by LPS. Cultures mouse microglia or astroglia cells were treated with LPS presence absence COX-2 inhibitors, production was measured ELISA. Cells PGE2, effect LPS-induced expression TNF-α...
CD69 is an immunomodulatory molecule induced during lymphocyte activation. Following stroke, T-lymphocytes upregulate but its function unknown.We investigated whether was involved in brain damage following ischemic stroke.We used adult male mice on the C57BL/6 or BALB/c backgrounds, including wild-type and CD69-/- mice, CD69+/+ lymphocyte-deficient Rag2-/- generated chimeric mice. We ischemia by transient permanent middle cerebral artery occlusion. measured infarct volume, assessed...
Abstract Progress in neuroscience research hinges on technical advances visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require fixation (electron microscopy), do not have cellular resolution (magnetic resonance imaging) or only give a fragmented view (fluorescence microscopy). Here, we show how regular light microscopy together fluorescence labeling of the interstitial fluid extracellular space provide comprehensive optical...
Isolated macrodontia, consisting of the gigantism a single tooth, is an extremely rare condition. Only 16 cases isolated macrodontia mandibular second premolars have been reported to date. Although aetiology this phenomenon remains unknown, many authors related it control apoptotic process, leading patterning and size dental cusps. There not clear genetic inheritance pattern since only two those correspond close relatives. To our knowledge, there no reports in archaeological remains. Cova...
Brain CD11c+ cells share features with microglia and dendritic (DCs). Sterile inflammation increases brain but their phenotype, origin, functions remain largely unknown. We report that after cerebral ischemia, attract DCs to the inflamed astroglia produce Flt3 ligand supporting development expansion of cells. in are a complex population derived from proliferating infiltrating DCs, including major subset OX40L+ conventional cDC2, also cDC1, plasmacytoid monocyte-derived DCs. Despite sharing...
Abstract Progress in neuroscience research hinges on technical advances visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require fixation, do not have cellular resolution or only give a fragmented view. Here, we show how regular light microscopy together fluorescence labeling of the interstitial fluid extracellular space provide comprehensive optical access real-time to anatomical complexity dynamics tissue.