A5085224236- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Nanofabrication and Lithography Techniques
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Advanced Biosensing Techniques and Applications
- Bacterial Genetics and Biotechnology
- Herpesvirus Infections and Treatments
- Platelet Disorders and Treatments
- Viral Infections and Immunology Research
- Image Processing Techniques and Applications
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Hematopoietic Stem Cell Transplantation
- Sphingolipid Metabolism and Signaling
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
- interferon and immune responses
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Neuroinflammation and Neurodegeneration Mechanisms
Instituto de Salud Carlos III
2012-2025
Centro Nacional de Microbiologia
2012-2025
Villanova University
2013
Abstract We have recently demonstrated that genetic inactivation of EWSR1 : FLI1 by CRISPR/Cas9, successfully blocks cell proliferation in a model Ewing sarcoma. However, CRISPR/Cas9-mediated gene editing can exhibit off-target effects, and thus, precise regulation Cas9 expression target cells is essential to develop gene-editing strategies inactivate sarcoma cells. In this study, we demonstrate be specifically expressed when located downstream promoter consisting GGAA repeats consensus TATA...
CD69 is an immunomodulatory molecule induced during lymphocyte activation. Following stroke, T-lymphocytes upregulate but its function unknown.We investigated whether was involved in brain damage following ischemic stroke.We used adult male mice on the C57BL/6 or BALB/c backgrounds, including wild-type and CD69-/- mice, CD69+/+ lymphocyte-deficient Rag2-/- generated chimeric mice. We ischemia by transient permanent middle cerebral artery occlusion. measured infarct volume, assessed...
CD69 is rapidly upregulated on T cells upon activation. In this work we show that also the case for expression dendritic (DC). Thus, kinetics of both cell types reminiscent one costimulatory molecules. Using mouse models transgenic cells, aimed at evaluating effect monoclonal antibody (MAb)-based targeting and gene deficiency expressed by either DC or extent antigen (Ag)-specific priming, which could be result a putative role in costimulation as well maturation Ag-processing presentation....
During the host response to viral infection, transmembrane CD69 protein is highly upregulated in all immune cells. We have studied role of murine vaccinia virus (VACV) and we report that absence enhances protection against VACV at both short long times postinfection immunocompetent immunodeficient mice. Natural killer (NK) cells were implicated increased infection control, since differences greatly diminished when NK depleted. This was not based on an altered cell reactivity, did affect...
In this study, we demonstrate the influence of human and mouse anti-CD69 therapies on immune response to VACV infection. We report that targeting CD69 increases leukocyte numbers in secondary lymphoid organs during infection improves capacity clear viral Targeting gamma interferon (IFN-γ)- tumor necrosis factor alpha (TNF-α)-producing NK T cells. mice expressing CD69, treatment with an MAb produces cytokine production, survival, proliferation mediated part by mTOR signaling. These results,...
The immune regulatory receptor CD69 is expressed upon activation in all types of leukocytes and strongly regulated at the transcriptional level. We previously described that, addition to promoter, there are four conserved noncoding regions (CNS1-4) upstream promoter. Furthermore, we proposed that CNS2 main enhancer transcription. In present study, mapped transcription factor (TF) binding sites (TFBS) from ChIP-seq databases within CNS2. Through luciferase reporter assays, defined a ~60 bp...
The CD69 gene encodes a C-type lectin glycoprotein with immune regulatory properties which is expressed on the cell surfaces of all activated hematopoietic cells. activation kinetics differ by developmental stage, linage and activating conditions, these differences have been attributed to participation complex networks. An evolutionarily conserved element, CNS2, located 4kb upstream transcriptional start site, has proposed as major candidate governing program. To investigate function human...
Human respiratory syncytial virus (HRSV) is the most common cause of severe infections in infants and young children, often leading to hospitalization. In addition, this poses a serious health risk immunocompromised individuals elderly. HRSV also major nosocomial hazard healthcare service units for patients all ages. Therefore, development antiviral treatments against global priority. study, mitoxantrone, synthetic anthraquinone with previously reported vitro antiprotozoal activities,...
Event Abstract Back to CD69 expression and leukocyte distribution in CD69-BAC transgenic mice Teresa Laguna1, 2*, Laura Notario1 Pilar Lauzurica1, 2 1 Instituto de Salud Carlos III, Spain Universitat Barcelona, Expressed on almost every leukocyte, function has been related co-stimulation lymphocytes, inhibition of pro-inflammatory responses leukocytes. Here we give new clues the complex regulation its effects immune system. We observed alterations several populations' CD69-overexpressing...
Event Abstract Back to RUNX transcription factors regulate the transcriptional activity of CD69 Teresa Laguna1, 2*, Laura Notario1 and Pilar Lauzurica1, 2 1 Instituto de Salud Carlos III, Spain Universitat Barcelona, receptor is considered a universal marker leukocyte activation; however, it immediately expressed after lymphocyte activation appears on other surfaces at later times. It has been also related with expression migration controller molecule S1P1, revealing an dependent...
<strong>Publisher's Note:</strong> This conference presentation, originally published on 4/19/17, was withdrawn per author request. The immune system is a very complex that comprises network of genetic and signaling pathways subtending interacting cells. location the cells in network, along with gene products they interact with, rules behavior system. Therefore, there great interest understanding properly role cell such networks to increase our knowledge response. In order acquire better...
Laser bioprinting is a powerful tool in many biological fields due to its versatility placing and construct different geometries of materials. The high accuracy non-destructive nature this method can be applied the study complex systems. In particular, single cell laser helps understand relationships between cells their local environment. Immunology transversal field that governed by network genetic signalling pathways subtending interacting cells. context, mobility along with situation gene...