A5027266976- Topic Modeling
- Natural Language Processing Techniques
- Cardiac Ischemia and Reperfusion
- Cardiac Imaging and Diagnostics
- Chemotherapy-induced cardiotoxicity and mitigation
- Semantic Web and Ontologies
- Biomedical Text Mining and Ontologies
- Advanced MRI Techniques and Applications
- Immune Cell Function and Interaction
- Speech and dialogue systems
- Lymphoma Diagnosis and Treatment
- Bioinformatics and Genomic Networks
- Epigenetics and DNA Methylation
- Telomeres, Telomerase, and Senescence
- Cardiac electrophysiology and arrhythmias
- Adipose Tissue and Metabolism
- Glycosylation and Glycoproteins Research
- Atherosclerosis and Cardiovascular Diseases
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Cardiovascular Function and Risk Factors
- Lanthanide and Transition Metal Complexes
- Polymer Nanocomposite Synthesis and Irradiation
- Viral-associated cancers and disorders
- Nuclear Structure and Function
Spanish National Centre for Cardiovascular Research
2014-2025
Centro de Investigación en Red en Enfermedades Cardiovasculares
2017
Icahn School of Medicine at Mount Sinai
2017
Universidad Europea
2017
Cardiovascular Institute of the South
2017
Philips (Spain)
2017
Centro de Investigación Biomédica en Red
2017
Universitat Politècnica de València
2000-2013
Cardiovascular Research Center
2012
Anthracycline-induced cardiotoxicity is a major clinical problem, and early markers are needed. The purpose of this study was to identify doxorubicin-induced by serial multiparametric cardiac magnetic resonance (CMR) its pathological correlates in large animal model. Twenty pigs were included. Of these, 5 received biweekly intracoronary doxorubicin doses (0.45 mg/kg/injection) followed until sacrifice at 16 weeks. Another 3 A third group sacrificed after the dose. All groups underwent weekly...
It is widely accepted that edema occurs early in the ischemic zone and persists stable form for at least 1 week after myocardial ischemia/reperfusion. However, there are no longitudinal studies covering from very (minutes) to late (1 week) reperfusion stages confirming this phenomenon.This study sought perform a comprehensive imaging histological characterization of edematous reaction experimental ischemia/reperfusion.The population consisted 25 instrumented Large-White pigs (30 kg 40 kg)....
Post-ischemia/reperfusion (I/R) myocardial edema was recently shown to follow a consistent bimodal pattern: an initial wave of appears on reperfusion and dissipates at 24 h, followed by deferred that initiates days after infarction, peaking 1 week.This study examined the pathophysiology underlying this post-I/R edematous reaction.Forty instrumented pigs were assigned different infarction protocols. Edematous reaction evaluated water content quantification, serial cardiac magnetic resonance...
A number of metabolites have signaling properties by acting through G-protein-coupled receptors. Succinate, a Krebs cycle intermediate, increases after dysregulated energy metabolism and can bind to its cognate receptor succinate 1 (Sucnr1, or GPR91) activate downstream pathways. We show that Sucnr1 is highly expressed in the white adipose tissue (WAT) compartment mice regulates mass glucose homeostasis. Sucnr1(-/-) were generated, weight gain was monitored under basal nutritional stress...
The impact of cardioprotective strategies and ischemia duration on postischemia/reperfusion (I/R) myocardial tissue composition (edema, myocardium at risk, infarct size, salvage, intramyocardial hemorrhage, microvascular obstruction) is not well understood.To study the effect protective interventions temporal dynamics in a translational animal model I/R by use state-of-the-art imaging technology.Four 5-pig groups underwent different protocols: 40-minute (prolonged ischemia, controls),...
Abstract Aims Anthracycline-induced cardiotoxicity (AIC) is a serious adverse effect among cancer patients. A central mechanism of AIC irreversible mitochondrial damage. Despite major efforts, there are currently no effective therapies able to prevent AIC. Methods and results Forty Large-White pigs were included. In Study 1, 20 randomized 1:1 remote ischaemic preconditioning (RIPC, 3 cycles 5 min leg ischaemia followed by reperfusion) or pretreatment. RIPC was performed immediately before...
Abstract Aims The aim of this study was to changes in coronary microcirculation status during and after several cycles anthracycline treatment. Methods results Large-white male pigs (n=40) were included different experimental protocols (ExPr.) according cumulative exposure [0.45 mg/kg intracoronary (IC) doxorubicin per injection] follow-up: control (no doxorubicin); single injection sacrifice either at 48 h (ExPr. 1) or 2 weeks 2); 3 injections apart (low dose) 3) 12 4) third injection; five...
Hutchinson-Gilford progeria syndrome (HGPS) is an extremely rare genetic disorder for which no cure exists. The disease characterized by premature aging and inevitable death in adolescence due to cardiovascular complications. Most HGPS patients carry a heterozygous de novo LMNA c.1824C > T mutation, provokes the expression of dominant-negative mutant protein called progerin. Therapies proven effective HGPS-like mouse models have yielded only modest benefit clinical trials. To overcome gap...
LMNA-associated congenital muscular dystrophy is a currently incurable rare genetic disorder characterized by early-onset muscle weakness, dilated cardiomyopathy and respiratory failure, resulting from mutations in the LMNA gene. In this study, we assessed potential of CRISPR-mediated strategy to eliminate mutant allele Lmna c.745C>T, p.R249W using mutation specific guide (sg745T). Results R249W-mutation-carrying cellular models showed activity Cas9/sg745T complex towards allele. This...
Background and Aims: Proliferation of arterial smooth muscle cells (SMCs) their modulation to alternative mesenchymal phenotypes is a central mechanism in the growth atherosclerotic lesions. The underlying processes have been studied extensively mouse models, but detailed analysis when where modulated SMCs accumulate human atherosclerosis lacking. present study mapped SMC subtypes during progression coronary explored associations with disease carotid plaques. Methods: Multiplex...
Myocardial ischemia-reperfusion (IR) injury may result in cardiomyocyte dysfunction. Mitochondria play a critical role recovery after IR injury. The mitochondrial uncoupling protein 3 (UCP3) has been proposed to reduce reactive oxygen species (ROS) production and facilitate fatty acid oxidation. As both mechanisms might be protective following injury, we investigated functional, structural, metabolic cardiac remodeling wild-type mice lacking UCP3 (UCP3-KO) IR. Results showed that infarct...
Left atrial (LA) remodeling after an acute myocardial infarction (MI) is poorly characterized regarding its determinants or effect on ischemic mitral regurgitation (MR) development. The purpose of this study was: 1) to compare LA structural in experimental MI swine models recapitulating the effects left ventricular (LV) dysfunction, MR, and (LAI); 2) analyze how influences MR Three were generated: proximal circumflex (LCx) coronary artery occlusion involving branch (LAI group); LCx not (LCx...
The vertiginous increase in the use of extracellular vesicles and especially exosomes for therapeutic applications highlights necessity advanced techniques gaining a deeper knowledge their pharmacological properties. Herein, we report novel chemical approach robust attachment commercial fluorescent dyes to exosome surface with covalent binding. applicability methodology was tested on milk cancer cell-derived (from U87 B16F10 cells). We demonstrated that labeling did not modify original...
Dyskeratosis congenita is an inherited disease caused by mutations in genes coding for telomeric components. It was previously reported that expression of a dyskerin-derived peptide, GSE24.2, increases telomerase activity, regulates gene and decreases DNA damage oxidative stress dyskeratosis patient cells. The biological activity short peptides derived from GSE24.2 tested one them, GSE4, probed to be active, further characterized this article. Expression eleven amino acids long peptide...
Amphibian-like ranaviruses include pathogens of fish, amphibians, and reptiles that have recently evolved from a fish-infecting ancestor. The molecular determinants host range virulence in this group are largely unknown, currently fish infection models lacking. We show European sheatfish virus (ESV) can productively infect zebrafish, causing lethal pathology, describe method for the generation recombinant ESV, establishing useful model study ranavirus infections.
We introduce a simple method to build Lexicalized Hidden Markov Models (L-HMMs) for improving the precision of part-of-speech tagging. This technique enriches contextual Language Model taking into account set selected words empirically obtained. The evaluation was conducted with different lexicalization criteria on Penn Treebank corpus using TnT tagger. obtained about 6% reduction tagging error, an unseen data test, without reducing efficiency system. have also studied how use linguistic...
This article has been retracted