A5014411379- Cardiac Ischemia and Reperfusion
- Physiological and biochemical adaptations
- Mitochondrial Function and Pathology
- Cardiac Valve Diseases and Treatments
- Aortic Disease and Treatment Approaches
- Cardiac Arrest and Resuscitation
- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Immune Response and Inflammation
- Infective Endocarditis Diagnosis and Management
- Aquaculture Nutrition and Growth
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Molecular Biology Techniques and Applications
- Zebrafish Biomedical Research Applications
- Neuroscience of respiration and sleep
- RNA Research and Splicing
- Cardiovascular Function and Risk Factors
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer, Hypoxia, and Metabolism
- Ion Transport and Channel Regulation
- Cardiac Imaging and Diagnostics
- ATP Synthase and ATPases Research
- Connective tissue disorders research
- Electrolyte and hormonal disorders
University of Oslo
2016-2025
Oslo University Hospital
2007-2016
ITMO University
2016
Institutt for Eksperimentell Medisinsk Forskning
2008-2011
Institute of Clinical Research
2010
Ossification is a tightly regulated process, performed by specialized cells called osteoblasts. Dysregulation of this process may cause inadequate or excessive mineralization bones ectopic calcification, all which have grave consequences for human health. Understanding osteoblast biology help to treat diseases such as osteogenesis imperfecta, calcific heart valve disease, osteoporosis, and many others. Osteoblasts are bone-building mesenchymal origin; they differentiate from progenitors,...
Like most vertebrates, humans die within minutes when deprived of molecular oxygen (anoxia), in part because cardiac failure. In contrast, some freshwater turtles can survive anoxia for months at low temperatures, but to do so, they drastically suppress activity and autonomic cardiovascular control. Although Carassius carassius, the crucian carp, shares this tolerance, we show that it has a unique ability among vertebrates retain normal performance regulation least 5 days anoxia. These...
Abstract Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to survive prolonged periods without oxygen by producing ethanol their end-product. Here we show this has been made possible evolution pyruvate decarboxylase, analogous in brewer’s yeast first described vertebrates, addition alcohol...
Objective: Hydrogen sulphide (H2S) protects the heart against ischaemia–reperfusion injury caused by low flow or local ischaemia. We hypothesised that: (1) H2S global of heart, (2) plays a mechanistic role in ischaemic preconditioning, and (3) acts phosphorylation protein kinases. Methods: Isolated, perfused rat hearts were used two series. Series 1: group 1.1 (n = 10), 40 min ischaemia 120 reperfusion, 1.2 7), like except that μM NaHS was added to perfusate during stabilisation throughout...
Background: Aortic valve calcification is an active proliferative process, where interstitial cells of the transform into either myofibroblasts or osteoblast-like causing deformation, thickening cusps and finally stenosis. This process may be triggered by several factors including inflammation, mechanical stress interaction with certain components extracellular matrix. The matrix different on two sides leaflets. We hypothesize that inflammation stimulate osteogenic differentiation human...
Abstract Aims Acute myocardial infarction causes lethal cardiomyocyte injury during ischaemia and reperfusion (I/R). Histones have been described as important Danger Associated Molecular Proteins (DAMPs) in sepsis. The objective of this study was to establish whether extracellular histone release contributes infarction. Methods results Isolated, perfused rat hearts were subject I/R. Nucleosomes histone-H4 detected early reperfusion. Sodium-β-O-Methyl cellobioside sulfate (mCBS), a newly...
Common for major surgery, multitrauma, sepsis, and critical illness, is a whole-body inflammation. Tissue injury able to trigger generalized inflammatory reaction. Cell death causes release of endogenous structures termed damage associated molecular patterns (DAMPs) that initiate sterile Mitochondria are evolutionary endosymbionts originating from bacteria, containing similar bacteria. These mitochondrial DAMPs (mDAMPs). Mitochondrial debris released into the extracellular space or...
ABSTRACT Background Sepsis is associated with myocardial injury and early mortality. The innate immune receptor Toll‐like 4 (TLR4) can recognize pathogen‐associated‐molecular‐patterns (PAMPs) damage‐associated molecular patterns (DAMPs); the latter are released during tissue injury. We hypothesized that TLR4 inhibition reduces proinflammatory signaling cytokine release in: (1) LPS or Escherichia coli ‐treated isolated mouse heart; (2) LPS‐treated primary adult cardiomyocytes; (3) heart...
We investigated whether two kinases critical for survival during periods of energy deficiency in anoxia-intolerant mammalian species, AMP-activated kinase (AMPK), and protein B (AKT), are equally important hypoxic/anoxic the extremely anoxia-tolerant crucian carp (Carassius carassius). report that phosphorylation AMPK AKT heart brain showed small changes after 10 days severe hypoxia (0.3 mg O2/l at 9 degrees C). In contrast, anoxia exposure (0.01 8 C) substantially increased but decreased...
Valve interstitial cells (VICs) are crucial in the development of calcific aortic valve disease. The purpose present investigation was to compare phenotype, differentiation potential and stem cell-like properties from calcified healthy valves. VICs were isolated human Calcification induced with osteogenic medium. Unlike valves, valves cultured without medium stained positively for calcium deposits Alizarin Red confirming their phenotype. Stimulation increased calcification (p = 0.02), but...
The crucian carp ( Carassius carassius ) tolerates anoxia for days to months depending on temperature. During episodes of stress, heat shock proteins (HSPs) are important limiting cellular damage, mainly by ensuring protein function. Accordingly, we hypothesized that would change the expression HSPs and this response be temperature dependent. Real-time RT-PCR was used investigate effects 1 7 (A1 A7) HSP70a, HSP70b, HSC70, HSP90, HSP30 in brain heart 8°C- 13°C-acclimated carp. In general, all...
High-doses of anabolic-androgenic steroids (AAS) is efficient for building muscle mass, but pose a risk cardiovascular side effects. Little known the effect AAS on vasculature, previous findings suggest unfavorable alterations in vessel walls and vasoreactivity. Here, long-term vascular function morphology were examined male weightlifters, mimicking animal model. Arterial elasticity tested with ultrasound, pulse wave velocity (PWV) carotid intima media thickness (cIMT) 56 current users, 67...
Myocardial ischemia-reperfusion (IR) injury may result in cardiomyocyte dysfunction. Mitochondria play a critical role recovery after IR injury. The mitochondrial uncoupling protein 3 (UCP3) has been proposed to reduce reactive oxygen species (ROS) production and facilitate fatty acid oxidation. As both mechanisms might be protective following injury, we investigated functional, structural, metabolic cardiac remodeling wild-type mice lacking UCP3 (UCP3-KO) IR. Results showed that infarct...
Very few vertebrates survive without oxygen (anoxia) for more than a minutes. Crucian carp (Carassius carassius) are one example, surviving months of anoxia at low temperatures, and we hypothesised that they maintain mitochondrial membrane potential function. Isolated crucian cardiomyocytes indeed maintained after blocking complex IV the electron transport system with cyanide, while those anoxia-intolerant trout depolarised. When complexes I-III were inhibited, mitochondria depolarised,...
Background Several beneficial effects have been demonstrated for secretogranin II (SgII) in non-cardiac tissue. As cardiac production of chromogranin A and B, two related proteins, is increased heart failure (HF), we hypothesized that SgII could play a role cardiovascular pathophysiology. Methodology/Principal Findings was characterized post-myocardial infarction (HF) mouse model, functional properties explored experimental models, circulating levels measured mice patients with stable HF...
Crucian carp (Carassius carassius) survive without oxygen for several months, but it is unknown whether they are able to protect themselves from cell death normally caused by the absence, and particularly return, of oxygen. Here, we quantified in brain tissue crucian exposed anoxia re-oxygenation using terminal deoxy-nucleotidyl transferase dUTP nick-end labelling (TUNEL) assay, proliferation immunohistochemical staining proliferating nuclear antigen (PCNA) as well PCNA mRNA expression. We...