A5049241346- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Regulation of Appetite and Obesity
- Exercise and Physiological Responses
- Spine and Intervertebral Disc Pathology
- Muscle Physiology and Disorders
- Lipid metabolism and biosynthesis
- Pain Mechanisms and Treatments
- Circadian rhythm and melatonin
- Diet and metabolism studies
- Biochemical Analysis and Sensing Techniques
- NF-κB Signaling Pathways
- Cardiac Ischemia and Reperfusion
- Infection Control and Ventilation
- Neurobiology and Insect Physiology Research
- Thermoregulation and physiological responses
- Ion Channels and Receptors
- Photosynthetic Processes and Mechanisms
- Pregnancy and preeclampsia studies
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Pregnancy-related medical research
- Extracellular vesicles in disease
- Antioxidant Activity and Oxidative Stress
- Neuroscience of respiration and sleep
National Institute of Occupational Health
2015-2024
Nofer Institute of Occupational Medicine
2022
University of Oslo
2007-2021
In addition to generating movement, skeletal muscle may have a function as secretory organ. The aim of the present study was identify novel proteins with signaling capabilities secreted from cells. IL-7 detected in media conditioned by primary cultures human myotubes differentiated satellite cells, and concentrations increased incubation time. By immunoblotting real-time RT-PCR expression confirmed at both protein mRNA levels. Furthermore, immunofluorescence specific antisera, multinucleated...
Adipokines are predominantly secretory protein hormones from adipose tissue but may also originate in placenta and other organs. Cross-sectionally, we monitored maternal plasma concentration of adiponectin, resistin, leptin their mRNA expression abdominal subcutaneous preeclamptic (PE; n = 15) healthy pregnant (HP; 23) women undergoing caesarean section. The study groups were similar age BMI, whereas HOMA-IR tended to be higher the PE group. In fasting samples, group had concentrations...
Regular physical activity protects against several types of diseases. This may involve altered secretion signaling proteins from skeletal muscle. Our aim was to identify the most abundantly secreted in cultures human muscle cells and monitor their expression muscles strength-training individuals. A total 236 were detected by proteome analysis medium conditioned cultured myotubes, which narrowed down identification 18 classically expressed muscle, using SignalP 3.0 Human Genome Expression...
Adipose tissue secretes a wide range of hormones named adipokines, and these may play role in obesity-related inflammation. Adiponectin is an exceptional adipokine because low plasma concentrations are associated with obesity, type 2 diabetes, cardiovascular diseases. It has been observed that adiponectin elevated during inflammatory conditions like preeclampsia arthritis. Nuclear factor-κB (NF-κB) essential transcription factor for expression inflammation-related proteins. We have used U937...
The pivotal role of liver X receptors (LXRs) in the metabolic conversion cholesterol to bile acids mice is well established. More recently, LXRα promoter has been shown be under tight regulation by peroxisome proliferator-activated (PPARs), implying a for mediating interplay between and fatty acid metabolism. We have studied LXR fat cells demonstrate that regulated during adipogenesis augments accumulation mature adipocytes. expression murine 3T3-L1 adipocytes as human was up-regulated...
Expression of the gene encoding resistin, a low molecular weight protein secreted from adipose tissue postulated to link obesity and type II diabetes, was examined in 3T3-L1 adipocytes. Resistin mRNA detected cells by day 3 following induction differentiation into adipocytes; 4 level resistin peaked remained high. The PPARgamma activators, rosiglitazone or darglitazone, reduced mRNA. Dexamethasone upregulated level, but no effect observed with beta(3)-adrenoceptor agonist, BRL 37344. A...
Background. Adiponectin is an adipose tissue‐derived protein counteracting insulin resistance and inflammation. We have compared women with gestational diabetes mellitus (GDM; n = 22) normal pregnancies (controls; 29) to evaluate whether adiponectin represents a link between endocrine function of tissue the development during pregnancy. Methods. The participants were categorized according their prepregnancy body mass index (BMI) into two classes: BMI < 25 25. Plasma concentrations...
Supplementation with n-3 polyunsaturated fatty acids (PUFA) for 6 weeks did not alter plasma leptin concentrations in male smokers. Changes dietary intake of saturated (FA) correlated positively, whereas changes the PUFA negatively to levels. A 3-week PUFA-enriched diet, as compared a lard-enriched induced lower concentration and reduced mRNA expression rat epididymal adipose tissue. In human throphoblast cell line (BeWo), had dose- time-dependent effect on expression. One mM...
BackgroundMyocardial ischemia is associated with alterations in cardiac metabolism, resulting decreased fatty acid oxidation and increased lipid accumulation. Here we investigate how myocardial content dynamics affect the function of ischemic heart, focus on role droplet protein perilipin 5 (Plin5) pathophysiology ischemia.Methods resultsWe generated Plin5−/− mice found that Plin5 deficiency dramatically reduced triglyceride heart. Under normal conditions, maintained a close to heart by...
Background All-trans retinoic acid (atRA), an active derivative of vitamin A, regulates cell differentiation, proliferation and cardiac morphogenesis via transcriptional activation receptors (RARs) acting on response elements (RARE).We hypothesized that the (RA) signalling pathway is activated in myocardial ischemia postischemic remodelling. Methods Findings Myocardial infarction was induced through ligating left coronary artery mice. In vivo RARs measured by imaging RARE-luciferase reporter...
Abstract Background Previous findings have demonstrated that lumbar radicular pain after disc herniation may be associated with up-regulation of inflammatory mediators. In the present study we examined possible role extracellular microRNAs (miRs) in this process. Methods Single unit recordings, isolation exosome-like vesicles, electron microscopy, nanoparticle tracking analysis, western blot analysis and qPCR were used rats to demonstrate effect nucleus pulposus (NP) applied onto dorsal...
The resistin gene is expressed in adipocytes and encodes a protein proposed to link obesity type 2 diabetes. Increased plasma FFA associated with insulin resistance. We examined the effect of separate FFAs on expression mRNA cultured murine 3T3-L1 adipocytes. tested did not increase expression, whereas both arachidonic acid (AA) eicosapentaenoic (EPA) reduced levels. AA was by far most potent FFA, reducing levels approximately 20% control at 60-250 muM concentration. Selective inhibitors...
The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of five perilipins in human skeletal muscle relation to fatty acid supply, exercise and energy balance. observed that all were expressed biopsies with highest levels 2, 4 5. Cultured myotubes predominantly 2 3. In vitro, incubation acids enhanced 1, 4. vivo, low fat diet increased 3 Endurance training, but not strength Perilipin 1 correlated positively body mass, whereas none associated insulin...
Coronary atherosclerosis can lead to myocardial infarction, and secondarily post-infarct remodelling heart failure. Retinoic acid (RA) influences cell proliferation. We hypothesized that RA could influence gene expression proliferation of cardiovascular cells. Left ventricular biopsies from patients with end-stage failure due coronary artery disease (CAD) or dilated cardiomyopathy were investigated for the content metabolites using liquid chromatography mass spectrometry (LC-MS/MS), compared...
The importance of adipose tissue in health as well disease has been demonstrated several studies recently, and it become appropriate to use the term 'adipose organ' when referring a whole. obesity epidemic, with marked increase incidence metabolic syndrome leading diabetes type 2 cardiovascular complications, stimulated considerable interest biology. Moreover, different species have shown that limited energy intake is associated less inflammation, improved biomarkers longevity. In addition,...
Low-grade inflammation is linked to metabolic syndrome and obesity. We studied the effects of weight loss dietary composition on serum concentrations biomarkers adipokines.Men women (n=181) aged 30-65 years with a body mass index (BMI) 28-40 kg/m(2) (28-35 for women) one or more components were randomized follow two hypocaloric diets, low-fat low-glycemic-load diet 3 months. Blood samples taken pre- postintervention. Serum interleukin-6 (IL-6), tumor necrosis factorα (TNF-α), plasminogen...
Background. Adiponectin is an adipose tissue-derived protein counteracting insulin resistance and inflammation. We have compared women with gestational diabetes mellitus (GDM; n = 22) normal pregnancies (controls; 29) to evaluate whether adiponectin represents a link between endocrine function of tissue the development during pregnancy. Methods. The participants were categorized according their prepregnancy body mass index (BMI) into two classes: BMI < 25 25. Plasma concentrations...