A5045184605- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Genetic and phenotypic traits in livestock
- CRISPR and Genetic Engineering
- Renal and related cancers
- Bioenergy crop production and management
- Ovarian function and disorders
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Tissue Engineering and Regenerative Medicine
- Ruminant Nutrition and Digestive Physiology
- Virus-based gene therapy research
- Xenotransplantation and immune response
- RNA Research and Splicing
- Effects of Environmental Stressors on Livestock
- Viral Infectious Diseases and Gene Expression in Insects
- Reproductive System and Pregnancy
- Vector-Borne Animal Diseases
- Genetically Modified Organisms Research
- Reproductive biology and impacts on aquatic species
Aarhus University
2013-2022
Frederiksberg Hospital
2015
University of Copenhagen
2015
Danish Cattle Research Centre
1994-2008
Unisense (Denmark)
2006-2007
Instituto de Ciencias Agrarias
1998-2006
University of Illinois Urbana-Champaign
2004
University of Georgia
2004
University of Guelph
2004
Scientific and Technological Research Council of Turkey
2004
Although cryopreservation of certain mammalian embryos is now a routine procedure, considerable differences efficiency exist depending on stage, species and origin (in vivo or in vitro produced). Factors that are suspected to cause most these the amount intracellular lipid droplets different microtubular structure leading chilling injury as well volume/surface ratio influencing penetration cryoprotectants. A new approach, Open Pulled Straw (OPS) method, which renders very high cooling...
Culture of mammalian zygotes individually and in small groups results lower developmental rates than culture large groups. Zona-free also have impaired potential current systems. This paper describes a new approach to resolve the problems, Well (WOW) system. Small wells (WOWs) were formed four-well dishes by melting bottom with heated steel rods. The WOWs then rinsed, filled medium, embryos placed into WOWs. To test value WOW system 3 x factorial experiment was performed. Bovine presumptive...
Cows and heifers were induced to superovulate by treatment with PMSG or FSH.The ultrastructural features of the oocytes related time LH peak progesterone/oestradiol-17\g=b\ ratios in follicular fluid.At 0\p=n-\2h after perivitelline space developed; at 9\p=n-\12h there was disconnection junctions between cumulus cell projections oolemma, concomitant breakdown oocyte nucleus; \m=~\15 h spatial rearrange- ments ooplasm (a) mitochondrial clusters from a peripheral an even dis- tribution (b)...
Fluorescence in situ hybridization with chromosome 6- and 7-specific probes was used to assess the extent of abnormalities developing bovine blastocysts at 7–8 days after insemination vivo or vitro. Interphase nuclei (N = 10 946) were analyzed from 151 produced vitro 28 recovered superovulated animals. This revealed that 72% (109 151) vitro-produced mixoploid, i.e., a mixture normal, diploid, polyploid cells. However, only small fraction total number cells chromosomally abnormal. Of...
A transgenic pig model of familial hypercholesterolemia can be used for translational atherosclerosis research.
The kinetics of the in vitro development early embryos from bovine zygotes derived and were compared, investigating effect serum during maturation fertilization (IVM-IVF) culture. Zygotes collected superovulated heifers or produced immature oocytes with without supplementation, cultured subsequently defined culture medium (SOFaaci) supplementation. Time-lapse images recorded every 0.5 h throughout period. More vivo- than vitro-derived developed to compact morula blastocyst stages (87% versus...
Widespread application of somatic cell cloning has been hampered by biological and technical problems, which include complicated time-consuming procedures requiring skilled labor. Recently, zona-free techniques have published with limited or no requirement for micromanipulators. The purpose the present work was to optimize certain steps micromanipulator-free (i.e., handmade) procedure, analyze morphology developing blastocysts, explain factors involved in high efficiencies observed....
The problems of sustaining placenta formation in embryos produced by nuclear transfer have emphasized the need for basic knowledge about epiblast and gastrulation bovine embryos. aims this study were to define stages post-hatching embryonic development analyse functional mechanisms germ-layer formation. Embryos developed vivo collected after slaughter from superovulated cows on days 9, 11, 14 21 insemination processed transmission electron microscopy (n = 26) or immunohistochemistry 27)...
Summary. Follicular oocytes (n = 81) collected from cattle at a local slaughterhouse were matured and fertilized in vitro. Of 27 ova 19 (70%) penetrated by spermatozoa 40/54 (74%) inseminated transferred surgically to the oviducts of synchronized heifer recovered non-surgical flushing uterine horns 6 days later. 40 15 (38%) morula, early blastocyst or diminutive morula stages. Culture vitro sustained further development all embryos 9 expanding expanded blastocysts. One pregnancy resulted...
DNA demethylation and remethylation are crucial for reprogramming of the differentiated parental/somatic genome in recipient ooplasm upon somatic cell nuclear transfer. Here, we analyzed methylation dynamics during porcine preimplantation development. Porcine vivo developed (IV), vitro fertilized (IVF), transfer (SCNT) parthenogenetically activated (PA) embryos were evaluated quantification at different developmental stages. Fertilized (IV IVF) one-cell stages lacked a substantial active...
The established causal genes in Alzheimer's disease (AD), APP, PSEN1, and PSEN2, are functionally characterized using biomarkers, capturing an vivo profile reflecting the disease's initial preclinical phase. Mutations SORL1, encoding endosome recycling receptor SORLA, found 2%–3% of individuals with early-onset AD, SORL1 haploinsufficiency appears to be for AD. To test whether can function as AD gene, we use CRISPR-Cas9-based gene editing develop a model Göttingen minipigs, taking advantage...
The objective of the present study was to investigate ultrastructural consequences vitrification bovine oocytes at metaphase II (MII) stage by so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved vitrification. After warming additional 2 culture, inseminated vitro. fixed transmission electron microscopy immediately after warming, 4 (i.e., post insemination [hpi]), 26 24 hpi), 74 72 hpi). Control nonvitrified oocytes) processed maturation 2, 22, hpi....
Although acceptable rates of blastocyst formation are achieved with in vitro production bovine embryos, several problems still compromise the subsequent development fetus and newborn, especially embryos originating from somatic cell nuclear transfer. Routinely, potential a conceptus is predicted either on quality or various parameters related to embryonic-fetal foster mother. These methods imprecise costly, highlighting need for more reliable practical evaluate early embryonic...