A5067901140- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Renal and related cancers
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Diet and metabolism studies
- Fungal Biology and Applications
- Adipose Tissue and Metabolism
- Plant Pathogens and Fungal Diseases
- Nutrition and Health in Aging
- Lipid metabolism and biosynthesis
- Ginseng Biological Effects and Applications
- Stroke Rehabilitation and Recovery
- Endoplasmic Reticulum Stress and Disease
- Genetic and phenotypic traits in livestock
- Plant Gene Expression Analysis
- Autophagy in Disease and Therapy
- Bioenergy crop production and management
- Land Rights and Reforms
- Reproductive System and Pregnancy
Huazhong Agricultural University
2011-2025
Nanjing Agricultural University
2014-2024
Shandong University
2004-2024
Changchun University of Chinese Medicine
2024
Loudi Central Hospital
2024
Shaanxi Provincial Land Engineering Construction Group
2024
Xi'an Jiaotong University
2024
China Land Surveying and Planning Institute
2024
Ministry of Natural Resources
2024
Shanghai Zhangjiang Laboratory
2022
DNA demethylation and remethylation are crucial for reprogramming of the differentiated parental/somatic genome in recipient ooplasm upon somatic cell nuclear transfer. Here, we analyzed methylation dynamics during porcine preimplantation development. Porcine vivo developed (IV), vitro fertilized (IVF), transfer (SCNT) parthenogenetically activated (PA) embryos were evaluated quantification at different developmental stages. Fertilized (IV IVF) one-cell stages lacked a substantial active...
The present study was designed to examine the effect of trichostatin A (TSA), an inhibitor histone deacetylase, on development porcine cloned embryos. Our results showed that treatment embryos derived from sow oocytes with 50 nM TSA for up 24 h after onset activation could significantly improve blastocyst yield compared control (46.4 ± 4.6% vs 17.7 4.9% treated and untreated embryos, respectively; p < 0.05), whereas similar cleavage rate total cell number per were observed. In order assess...
Abstract Polycystic ovarian syndrome (PCOS) is associated with hyperhomocysteinemia and polycystic ovaries (PCO) usually produce oocytes of poor quality. However, the intracellular mechanism linking oocyte quality remains elusive. In this study, isolated from healthy gilt was evaluated in vitro association one-carbon metabolism, mitochondrial DNA (mtDNA) methylation function. PCO demonstrated impaired polar body extrusion significantly decreased cleavage blastocyst rates. The distribution...
The effects of dietary garcinol on diarrhea and intestinal barrier function associated with its modulation gut microbiota in weaned piglets were investigated.One hundred forty four (Duroc × Yorkshire Landrace) from 16 pens (9 per pen) randomly divided into treatment groups: controls (CON) or those supplemented 200 mg/kg (LOW), 400 (MID), 600 (HIGH) diet garcinol. After 14-day trial, three pen chosen to collect plasma, tissue colonic digesta samples.We demonstrated for the first time that...
Summary Our knowledge of how the parental genomes interact to shape hybrid performance remains limited. This work established four maize populations and evaluated plant height (PH) in both populations, generating an extensive transcriptome translatome dataset. We conducted a genome‐wide association study, expression quantitative trait locus (eQTLs) mapping, transcriptome‐wide mapping (TWAS), allele‐specific analysis elucidate regulatory mechanisms underlying PH variation hybrids. QTLs,...
The weight-to-waist index (WWI) serves as an innovative metric specifically designed to assess central obesity. However, the relationship between WWI and prevalence of post-stroke depression (PSD) remains inadequately explored in literature. To elucidate PSD. Data from National Health Nutrition Examination Survey 2005 2018 were analyzed. Multivariable logistic regression models propensity score matching utilized investigate association PSD, with adjustments for potential confounders....
Cefquinome is a fourth generation cephalosporin with antimicrobial activity against gram negative and positive bacterial species, including Staphylococcus aureus. The aim of our study was to observe the ex-vivo cefquinome aureus strains by using bovine serum from intravenously treated cattle. kinetics were measured liquid chromatography UV detection. In vitro post antibiotic effects (PAEs) mutant prevention concentrations determined S. strain ATCC 12598. exhibited time-dependent killing...
Introduction Diabetes affects both the peripheral and central nervous systems. The aim of this study was to explore changes in brain activity response thermal stimuli diabetic patients with without neuropathy (DPN) using functional magnetic resonance imaging (fMRI). Methods A total 36 right-handed volunteers were enrolled: eight Type-2 diabetes mellitus DPN, 13 lacking DPN (NDPN patients), 15 healthy (HV). Blood oxygenation level-dependent baseline scans performed, first any stimuli, then...
Hand, foot and mouth disease (HFMD) is a rising public health problem has attracted considerable attention worldwide. The purpose of this study was to develop an optimal model with meteorological factors predict the epidemic HFMD.Two types methods, back propagation neural networks (BP) auto-regressive integrated moving average (ARIMA), were employed forecasting models, based on monthly HFMD incidences during 2009-2016 in Jiangsu province, China. Root mean square error (RMSE) absolute...
Understanding how skeletal muscle fiber proportions are regulated is essential for understanding function and improving the quality of mutton. While circular RNA (circRNA) has a critical in myofiber type transformation, specific mechanisms not yet fully understood. Prior evidence indicates that ubiquitin-specific peptidase 13 (circUSP13) can promote myoblast differentiation by acting as ceRNA, but its potential role switching still unknown. Herein, we found circUSP13 enhanced slow myosin...
An innovative technique, called the high hydrostatic pressure (HHP) treatment, has been recently reported to improve cryosurvival of gametes or embryos in certain mammalian species. The aim present study was investigate vitro and vivo developmental competence cryotolerance produced by handmade cloning (HMC) after treatment recipient oocytes. In vitro-matured porcine oocytes were treated with a sublethal 20 MPa (200 times greater than atmospheric pressure) recovered for either 1 2 h (HHP1...
Successful cryopreservation of porcine embryos offers a promising perspective in the fields agriculture, animal science, and human medical research. The objective present work was to establish system facilitating produced by somatic cell nuclear transfer (SCNT). Several key techniques including micromanipulator-based enucleation, noninvasive delipation, zona-free fusion, activation were combined with high efficiency. After partial zona digestion high-speed centrifugation, 89.8 ± 2.1% (mean...
Homocysteine (Hcy) is an intermediate in the one-carbon metabolism that donates methyl groups for methylation processes involved epigenetic gene regulation. Although poor oocyte quality polycystic ovarian syndrome (PCOS) patients associated with elevated Hcy concentration serum and follicular fluid, whether directly affects its mechanisms are poorly understood. Here we show treatment impaired developmental competence, indicated by significantly reduced survival rate, polar body extrusion...