A5004637660- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Skin and Cellular Biology Research
- Atherosclerosis and Cardiovascular Diseases
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Lipoproteins and Cardiovascular Health
- Cardiac Fibrosis and Remodeling
- DNA and Nucleic Acid Chemistry
- Cholinesterase and Neurodegenerative Diseases
- Machine Learning in Bioinformatics
- Reproductive Biology and Fertility
- Alzheimer's disease research and treatments
- Sleep and related disorders
- Hormonal and reproductive studies
- Nuclear Receptors and Signaling
- Plant Reproductive Biology
- RNA regulation and disease
- Chemokine receptors and signaling
- Viral Infections and Immunology Research
- Sleep and Wakefulness Research
- Retinal Development and Disorders
Aarhus University
2014-2024
Technical University of Denmark
2024
Karolinska Institutet
2023-2024
Aarhus University Hospital
1998-2023
Vitenparken
1997
A transgenic pig model of familial hypercholesterolemia can be used for translational atherosclerosis research.
The established causal genes in Alzheimer's disease (AD), APP, PSEN1, and PSEN2, are functionally characterized using biomarkers, capturing an vivo profile reflecting the disease's initial preclinical phase. Mutations SORL1, encoding endosome recycling receptor SORLA, found 2%–3% of individuals with early-onset AD, SORL1 haploinsufficiency appears to be for AD. To test whether can function as AD gene, we use CRISPR-Cas9-based gene editing develop a model Göttingen minipigs, taking advantage...
Hutchinson-Gilford progeria syndrome (HGPS) is an extremely rare genetic disorder for which no cure exists. The disease characterized by premature aging and inevitable death in adolescence due to cardiovascular complications. Most HGPS patients carry a heterozygous de novo LMNA c.1824C > T mutation, provokes the expression of dominant-negative mutant protein called progerin. Therapies proven effective HGPS-like mouse models have yielded only modest benefit clinical trials. To overcome gap...
An innovative technique, called the high hydrostatic pressure (HHP) treatment, has been recently reported to improve cryosurvival of gametes or embryos in certain mammalian species. The aim present study was investigate vitro and vivo developmental competence cryotolerance produced by handmade cloning (HMC) after treatment recipient oocytes. In vitro-matured porcine oocytes were treated with a sublethal 20 MPa (200 times greater than atmospheric pressure) recovered for either 1 2 h (HHP1...
Cardiac fibrosis contributes to the development of heart failure in pulmonary hypertension. We aimed assess and effects treatment with anti‐fibrotic agent pirfenidone pressure overload induced right ventricular (RV) failure. Wistar rat weanlings were randomized trunk banding (PTB) or sham surgery. One week after procedure, PTB rats into two groups either six weeks on standard chow mixed (700 mg/kg/day). RV hemodynamic evaluated by echocardiography, cardiac magnetic resonance imaging (MRI),...
Animal breeding via Somatic Cell Nuclear Transfer (SCNT) has enormous potential in agriculture and biomedicine. However, concerns about whether SCNT animals are as healthy or epigenetically normal conventionally bred ones raised the efficiency of cloning by is much lower than natural In-vitro fertilization (IVF). Thus, we have conducted a genome-wide gene expression DNA methylation profiling between phenotypically cloned pigs control two tissues (muscle liver), using Affymetrix Porcine array...
Abstract The sortilin-related receptor 1 (SORL1) gene, encoding the cellular endosomal sorting-related with A-type repeats (SORLA), is now established as a causal gene for Alzheimer’s disease. As latest addition to list of genes, pathophysiological effects and biomarker potential SORL1 variants remain relatively undiscovered. Metabolic dysfunction is, however, well described in patients disease used an imaging clinical diagnosis settings. To understand metabolic consequences loss-of-function...
Abstract Age-related alterations in the auditory system have been suggested to affect processing of temporal envelope amplitude modulations (AM) at different levels hierarchy, yet few studies used functional magnetic resonance imaging (fMRI) study this noninvasively humans with high spatial resolution. In study, we utilized sparse-sampling fMRI 3 Tesla (3T) investigate regional blood oxygenation level-dependent (BOLD) responses AM noise stimuli 65 individuals ranging age from 19 77 years. We...
Histidine‐rich glycoprotein (HRG) was purified from bovine plasma and the disulphide bridge arrangement established. Disulphide‐bridged peptides were obtained peptic tryptic degradation of native HRG. Twelve half‐cystine residues found in HRG (compared to sixteen cysteines human HRG), all involved formation six bridges connecting Cys‐1 Cys‐12, Cys‐2 Cys‐3, Cys‐4 Cys‐5, Cys‐6 Cys‐11, Cys‐7 Cys‐8, Cys‐9 Cys‐10. Additional sequence analysis 14 C‐carboxymethylated chymotryptic Staphylococcus...
Deficiency of apolipoprotein E (APOE) causes familial dysbetalipoproteinemia in humans resulting a higher risk atherosclerotic disease. In mice, APOE deficiency results severe atherosclerosis phenotype, but it is unknown to what extent this unique mice. study, was targeted Yucatan minipigs. APOE-/- minipigs displayed increased plasma cholesterol and accumulation B-48-containing chylomicron remnants on low-fat diet, which significantly accentuated upon feeding high-fat, high-cholesterol diet....
Treatment of prostate cancer often involves androgen deprivation therapy (ADT) by gonadotropin-releasing hormone (GnRH) receptor agonists, GnRH antagonists, or orchiectomy. ADT may increase the rate cardiovascular disease events, but recent clinical studies suggested that not all means carry same risk, raising possibility non-testosterone-mediated effects different forms on atherosclerosis. Here we compared atherosclerosis in intact and orchiectomized Apoe-deficient mice.Chow-fed mice were...
Background and aimsSmooth muscle cell (SMC) lineage cells in atherosclerosis flow cessation-induced neointima are oligoclonal, being recruited from a tiny fraction of medial SMCs that modulate proliferate. The present study aimed to investigate the clonal structure SMC healing more severe arterial injury.MethodsArterial injury (wire, stretch, partial ligation) was inflicted on right carotid artery mice with homozygous, SMC-restricted, stochastically recombining reporter transgenes produced...
Abstract: Epidermolysis bullosa simplex (EBS) is a group of autosomal dominantly inherited skin disorders characterized by the development intra‐epidermal blisters on mild mechanical trauma. The three major clinical subtypes (Weber‐Cockayne, Koebner and Dowling‐Meara) are all caused mutations in either keratin 5 (KRT5) or 14 (KRT14) gene. Previously, we identified novel KRT14 missense Danish EBS patients associated with different forms ( 1 ). represent full spectrum classical subtypes. In...