A5017420079- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- Metabolism and Genetic Disorders
- Transgenic Plants and Applications
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Skin and Cellular Biology Research
- Biochemical and Molecular Research
- Growth Hormone and Insulin-like Growth Factors
- Chromosomal and Genetic Variations
- Animal Behavior and Reproduction
- Genetics and Neurodevelopmental Disorders
- Mesenchymal stem cell research
- Retinal Development and Disorders
- Genetic Neurodegenerative Diseases
- MicroRNA in disease regulation
- Muscle Physiology and Disorders
- Avian ecology and behavior
- Animal Nutrition and Physiology
- Molecular Biology Techniques and Applications
- Diet and metabolism studies
San Diego Zoo Institute for Conservation Research
2010-2023
Aurora University
2023
Aarhus University
2009-2022
Kennedy Center
2004-2009
Zoological Society of San Diego
2003-2007
Aarhus University Hospital
1994-2003
Aalborg University
1998
National Institutes of Health
1997
Rigshospitalet
1996
Institute of Human Genetics
1993
Abstract Dlk-1/Pref-1 was identified as a novel regulator of human skeletal stem cell differentiation. Dlk1/Pref-1 is expressed in bone and cultured osteoblasts, its constitutive overexpression led to inhibition osteoblast adipocyte differentiation marrow stromal cells. Introduction: Molecular control mesenchymal (hMSC) into osteoblasts adipocytes not known. In this study, we examined the role delta-like 1/preadipocyte factor-1 (Dlk1/Pref-1) regulating hMSCs. Materials Methods: As model for...
We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent (hiPS-MSCs). The hiPS-MSCs expressed markers (CD90, CD73 and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic chondrogenic lost pluripotency – as seen the loss of OCT3/4 TRA-1-81 tumorigenicity. However, these iPS-MSCs are still positive marker NANOG. further explored osteogenic potential in...
Abstract The ability to rapidly and reliably determine the sex of birds is very important for successful captive‐bird breeding programs, as well field research. Visual inspection adult sufficient sexually dimorphic species, but nestlings monomorphic species are difficult, if not impossible, by sight only. A method rapid extraction gDNA from blood, shell‐membrane blood vessels, fully grown feathers, using Chelex, PCR conditions determination sex‐specific bands in 47 (39 genera, 21 families,...
Tumor necrosis factor-alpha (TNF-alpha) is upregulated in psoriatic skin and represents a prominent target psoriasis treatment. The level of TNF-alpha-encoding mRNA, however, not increased skin, it remains unclear whether intervention strategies based on RNA interference (RNAi) are therapeutically relevant. To test this hypothesis the present study describes first vitro functional screening panel short hairpin RNAs (shRNAs) targeting human TNF-alpha mRNA and, next, transfer most potent shRNA...
We have used expression of human medium chain acyl-CoA dehydrogenase (MCAD) in Escherichia coli as a model system for dissecting the molecular effects two mutations detected patients with MCAD deficiency. demonstrate that R28C mutation predominantly affects polypeptide folding. The amounts active mutant enzyme produced could be modulated between undetectable to 100% wild-type control by manipulating level available chaperonins and growth temperature. For prevalent K304E mutation, however,...
Phage c31 integrase is a recombinase with the potential to perform site-specific integration into host chromosome. The enzyme mediates recombination between two different short sequences of DNA, attP and attB recognition sites. In mouse human genomes, phage plasmids bearing an site partial sequence identity attP, called pseudo Based on identification sites in genome it was estimated that total number 102 103. integrase-mediated gene delivery under investigation as novel tool for therapy....
Fat and bone metabolism are two linked processes regulated by several hormonal factors. Fetal antigen 1 (FA1) is the soluble form of dlk1 (delta-like 1), which a member Notch-Delta family. We previously identified FA1 as negative regulator marrow mesenchymal stem cell differentiation. Here, we studied effects circulating on fat mass in vivo generating mice expressing high serum levels (FA1 mice) using hydrodynamic-based gene transfer procedure. found that increased led to significant...
Abstract Background Strategies leading to the long‐term suppression of inappropriate ocular angiogenesis are required avoid need for repetitive monthly injections treatment diseases eye, such as age‐related macular degeneration (AMD). The present study aimed develop a strategy sustained repression vascular endothelial growth factor (VEGF), which is identified key player in exudative AMD. Methods We have employed short hairpin (sh)RNAs combined with adeno‐associated virus (AAV) delivery...
Lentivirus-based gene delivery vectors carrying multiple cassettes are powerful tools in transfer studies and therapy, allowing coexpression of therapeutic factors and, if desired, fluorescent reporters. Current strategies to express transgenes microRNA (miRNA) clusters from a single vector have certain limitations that affect transgene expression levels and/or titers. In this study, we describe novel design facilitates combined RNA- protein-based antiangiogenic as well reporter back-to-back...
Myotonic dystrophy type 1 (DM1) is caused by CUG triplet expansions in the 3′ UTR of dystrophia myotonica protein kinase (DMPK) messenger ribonucleic acid (mRNA). The etiology this multi-systemic disease involves pre-mRNA splicing defects elicited ability CUG-expanded mRNA to 'sponge' factors muscleblind family. Although nuclear aggregation CUG-containing mRNPs distinct foci a hallmark DM1, mechanisms their homeostasis have not been completely elucidated. Here we show that DEAD-box helicase,...
Context In the past decade, flow cytometry has become a useful tool for evaluating cellular viability characteristics non-domestic animals such as non-human primates, marine animals, and birds. This technology potential to vastly improve sperm-quality assessments, concentration counts cell sorting in more time-efficient reliable manner. Aims The study aimed validate efficacy of using amphibian sperm by comparing its results with those obtained through traditional means assessment. Methods...
Background: Chronic kidney disease occurs in approximately 11% of the adult population, and about 0.3% develops end-stage renal (ESRD) requiring replacement therapy. The Kidney Failure Risk Equation (KFRE) can predict 2 5-year risk dialysis patients with CKD stages 3 to 5. Having a tool ESRD within 2-5 years is value prioritize health resources for moderate high risk. method has been validated several other countries. Objective: To validate KFRE Norwegian population 3-5. Patients Methods: A...
The autosomal dominant form of familial neurohypophyseal diabetes insipidus (adFNDI) is a rare disease characterized by postnatal onset polyuria and deficient neurosecretion the antidiuretic hormone, arginine vasopressin (AVP). Since 1991, adFNDI has been linked to 31 different mutations gene that codes for vasopressin-neurophysin II (AVP-NPII) precursor. aims present study were relate clinical phenotype specific genotype molecular genetic effects most frequently reported mutation located at...
Abstract Background PhiC31 integrase facilitates efficient integration of transgenes into human and mouse genomes is considered for clinical gene therapy. However recent studies have shown that the enzyme can induce various chromosomal abnormalities in primary embryonic cells mammalian cell lines. The mechanisms involved are unknown, but it has been proposed attachment sites host genome recombine leading to translocations. Results We studied possible effects expression adult fibroblasts by...
Abstract Background Vascular endothelial growth factor (VEGF) is an angiogenic that plays a critical role in several diseases, including cancer, rheumatoid arthritis and diseases of the eye. Persistent regulation VEGF by expression small interfering RNAs targeting represents potential future strategy for treatment such diseases. As step toward this goal, present study combines potency VEGF‐targeted miRNA mimics, produced from cluster, with delivery adeno‐associated virus (AAV)‐based vectors....