A5060185413- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Cytomegalovirus and herpesvirus research
- Chronic Lymphocytic Leukemia Research
- Tuberculosis Research and Epidemiology
- Microfluidic and Bio-sensing Technologies
- COVID-19 Clinical Research Studies
- Multiple Sclerosis Research Studies
- Cell Image Analysis Techniques
- Acute Lymphoblastic Leukemia research
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- Cancer Immunotherapy and Biomarkers
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Lymphoma Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Point processes and geometric inequalities
- Blood disorders and treatments
- Radiopharmaceutical Chemistry and Applications
- Polyomavirus and related diseases
Aarhus University
2011-2024
Lund University
2017
Skåne University Hospital
2017
We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent (hiPS-MSCs). The hiPS-MSCs expressed markers (CD90, CD73 and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic chondrogenic lost pluripotency – as seen the loss of OCT3/4 TRA-1-81 tumorigenicity. However, these iPS-MSCs are still positive marker NANOG. further explored osteogenic potential in...
Abstract Plasmacytoid dendritic cells (pDC) are essential for immune competence. Here we show that pDC precursor differentiated from human CD34 + hematopoietic stem and progenitor (HSPC) has low surface expression of markers, limited induction type I interferon (IFN) IL-6 upon TLR7 TLR9 agonists treatment; by contrast, cGAS or RIG-I agonists-mediated activation is not altered. Importantly, after priming with II IFN, these pDCs attain a phenotype functional activity similar to peripheral...
TLR3 and TLR9 recognize the pathogen-associated microbial patterns dsRNA unmethylated DNA, respectively. The recent discovery that these receptors also endogenous ligands from necrotic material has drawn increased attention to their involvement in autoimmunity. Th cell cytokines IL-17A IL-21 have been assigned with pivotal roles regulation of such autoimmune diseases. is hallmark cytokine recently discovered proinflammatory subset T(H)17. By contrast, expression does not seem be limited a...
Hypogammaglobulinemia may be part of several different immunological or malignant conditions, and its origin is not always obvious. Furthermore, although autoimmune cytopenias are known to associated with common variable immunodeficiency (CVID) even precede signs immunodeficiency, this recognized. Despite novel insight into the molecular immunology areas uncertainty remain. In addition, full spectrum effects B cell depleting anti-CD20 antibody Rituximab has been fully explored. To our...
Abstract Insulin secretion from pancreatic beta-cells is dependent on zinc ions as essential components of insulin crystals, transporters are thus involved in the secretory process. Zip14 (SLC39a14) a importing protein that has an important role glucose homeostasis. knockout mice display hyperinsulinemia and impaired high conditions. Endocrine roles for have been established adipocytes hepatocytes, but not yet confirmed beta-cells. In this study, we investigated INS-1E beta-cell line. mRNA...
Objectives Cognitive impairment is common in multiple sclerosis (MS) and can have serious impact on social occupational functioning. Natalizumab reduces relapse rates, magnetic resonance imaging (MRI) lesions, progression of disability. Previous studies cognitive functioning not based inclusion performance criteria. The aim the present study was to determine any potential natalizumab-related effects MS patients performing below normal limits neuropsychological testing. Materials methods...
<b>Background:</b> Several studies have documented a substantial genetic component in the aetiology of allergic diseases and number atopy susceptibility loci been suggested. One these is 3q21, at which linkage to multiple phenotypes has reported. This region harbours <i>CD86</i> gene encoding costimulatory B7.2 protein. The system, consisting receptor proteins, cytokines associated factors, activates T cells regulates immune response upon allergen challenge. <b>Methods:</b> We sequenced...
A general hindrance to progress in adoptive cellular therapy is the lack of detailed knowledge fate transferred cells body recipient. In this study, we present a novel technique for tracking 124I-labeled situ, which combines high spatial resolution magnetic resonance imaging with sensitivity and accuracy positron emission tomography. We have used technique, together determination tissue radioactivity, flow cytometry, microscopy, characterize quantitate specific accumulation CD8+ T tumor...
Our genes are post-transcriptionally regulated by microRNAs (miRNAs) inducing translational suppression and degradation of targeted mRNAs. Strategies to inhibit miRNAs in a spatiotemporal manner desired cell type or tissue, at developmental stage, can be crucial for understanding miRNA function pushing forward as feasible rationale genetic treatment disease. For such purposes, RNA polymerase II (RNA Pol II)-transcribed tough decoy (TuD) inhibitors particularly attractive. Here, we...
Accumulation of T cells at the tumor is essential in cancer immunotherapy based on adoptive transfer tumor-specific cells. To gain further insight into accumulation process and to evaluate effect using different routes cell transfer, we investigated ovalbumin-specific CD8+ (OT-I) injected either intravenously (IV) or intraperitoneally (IP) mice carrying a subcutaneous ovalbumin-expressing melanoma line B16-OVA. Maximal adoptively transferred tissue was observed 5 days after injection,...
We have analysed the consequences of liposome mediated gene transfer into human primary epidermal keratinocytes and compared non-Epstein-Barr Virus (EBV) EBV based expression vectors that carry genes encoding Growth Hormone (hGH) or Enhanced Green Fluorescent Protein (EGFP). Different kinetics between non-EBV were revealed upon subcultivation hGH transfected keratinocytes. The with vector showed a rapid reduction in production. Although resulted more stable expression, this was also reduced...
Human herpesvirus 6B (HHV-6B) is the causative agent of common childhood febrile illness, exanthema subitum. The virus predominantly regarded as a T-cell tropic virus, although in reality it has ability to infect wide variety cell types including monocytes, macrophages and dendritic cells (DC). Although DC are important immune regulators, modulating effects HHV-6B on controversial. Here, we examine phenotypic functional consequences infection DC. addition immature led expression nuclear...
Isolation of multiple cell populations from limited starting material and with minimal influence on homeostasis viability are common requirements in both basic clinical research. Fluorescence-activated sorting (FACS) is the most commonly applied methodology majority instruments being based high pressure electrostatic deflection. A more recent technology a mechanical valve, operating at low pressure. In present work we compared two technologies by parallel small amounts peripheral blood...
Reactivation of human cytomegalovirus (HCMV) remains a serious problem in immunosuppressed individuals. To investigate whether change the immune status can be used as an earlier marker for HCMV reactivation than traditional PCR analysis, eight chronic lymphocytic leukemia (CLL) patients at risk due to commencement alemtuzumab (anti-CD52) treatment were longitudinally followed. Five series consecutive weekly blood samples immunophenotyped by flow cytometry cover both innate and adaptive...
Summary The exact disease state of chronic myeloid leukaemia ( CML ) patients in deep molecular remission is unknown, because even the most sensitive quantitative reverse transcription polymerase chain reaction qPCR methods cannot identify prone to relapse after treatment withdrawal. To elucidate this, CD 34 + stem cell and progenitor subpopulations were isolated by fluorescence‐activated sorting FACS ), their content residual Philadelphia positive P h cells was evaluated 17 (major response,...