A5042227938- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- Folate and B Vitamins Research
- Melanoma and MAPK Pathways
- Mitochondrial Function and Pathology
- Pulmonary Hypertension Research and Treatments
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Biochemical and Molecular Research
- Congenital Heart Disease Studies
- CAR-T cell therapy research
- Cancer-related gene regulation
- Metabolomics and Mass Spectrometry Studies
- Neonatal Health and Biochemistry
- Diet and metabolism studies
- Acute Myeloid Leukemia Research
- Cancer-related Molecular Pathways
- Porphyrin Metabolism and Disorders
- Genomic variations and chromosomal abnormalities
- Cutaneous Melanoma Detection and Management
- DNA Repair Mechanisms
- Genetic Syndromes and Imprinting
- Lymphoma Diagnosis and Treatment
- Bladder and Urothelial Cancer Treatments
Danish Cancer Society
2015-2025
University of Southern Denmark
2021-2024
Cancer Institute (WIA)
2023
University of Copenhagen
1994-2018
Herlev Hospital
1998-2018
University of Tübingen
2013
Odense University Hospital
1995-2011
Aarhus University
2011
University Hospital Heidelberg
2011
Karlsruhe Institute of Technology
2011
DNA methylation patterns are altered in numerous diseases and often correlate with clinically relevant information such as disease subtypes, prognosis drug response. With suitable assays after validation large cohorts, associations can be exploited for clinical diagnostics personalized treatment decisions. Here we describe the results of a community-wide benchmarking study comparing performance all widely used methods analysis that compatible routine use. We shipped 32 reference samples to...
// Arnaldur Hall 1 , 2 Kathrine Damm Meyle 3 Marina Krarup Lange Martin Klima May Sanderhoff 4 Christina Dahl 5 Cecilie Abildgaard Katrine Thorup Seyed Moein Moghimi Per Bo Jensen Jiri Bartek Guldberg and Claus Christensen Genome Integrity Unit, Danish Cancer Society Research Center, Denmark Centre for Pharmaceutical Nanotechnology Nanotoxicology, University of Copenhagen, Unit Dietary Studies Institute Preventive Medicine Bispebjerg Frederiksberg Hospitals, Seahorse Bioscience Europa, Diet,...
Abstract: The propensity of myelodysplastic syndrome (MDS) to transform into acute myeloid leukemia (AML) suggests the existence common pathogenic components for these malignancies. Here, four genes implicated in development AML were examined promoter CpG island hypermethylation cells from 37 patients with different stages MDS. Aberrant methylation was detected by polymerase chain reaction amplification bisulfite‐treated DNA followed denaturing gradient gel electrophoresis. highest rate...
Summary Mutations in DNMT 3A , the gene encoding DNA methyltransferase 3 alpha, have been identified as molecular drivers acute myeloid leukaemia ( AML ) with possible implications for minimal residual disease monitoring and prognosis. To further explore utility of mutations biomarkers we developed assays sensitive detection recurrent affecting residue R882. Analysis from 298 diagnostic samples revealed 45 cases (15%), which coincided NPM 1 FLT IDH . were stable 12 13 patients presenting...
To investigate incidence, clinicopathological features and prognosis of BRAF-mutated conjunctival melanoma in Denmark. Furthermore, to determine BRAF mutations paired premalignant lesions evaluate immunohistochemical V600E oncoprotein detection.Data from 139 patients with (1960-2012) were collected. Archived samples analysed for using droplet digital polymerase chain reaction (PCR). Results associated compared stainings.The overall incidence (0.5 cases/1 000 000/year) increased during the...
Most PCR assays for detection of 5-methylcytosine in genomic DNA entail a two-step procedure, comprising initial amplification and subsequent product analysis separate operations that usually require manual transfer. These methods generally provide information about methylation only few CpG dinucleotides within the target sequence.An in-tube assay is described integrates bisulfite-treated melting by using thermal cycler coupled to fluorometer (LightCycler). curves were acquired measuring...
Abstract Long-term cultures of telomerase-transduced adult human mesenchymal stem cells (hMSC) may evolve spontaneous genetic changes leading to tumorigenicity in immunodeficient mice (e.g., hMSC-TERT20). We wished clarify whether this unusual phenotype reflected a rare but dominant subpopulation or if the cell origin allowed most behave as cancer cells. Cultures hMSC-TERT20 strain at population doubling 440 were highly clonogenic (94%). From 110 single-cell clones expanded by 20 doublings,...
A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived two head and neck carcinomas, which expressed mutant genes, L9V/HLA-A2 specific restricted fashion. Thus, normal tolerance endogenously processed self-epitopes can be broken by peptide-specific priming. peptides might thus represent tumor associated target...
The molecular basis of methotrexate resistance was studied in human MDA-MB-231 breast cancer cells, which are inherently defective uptake and lack expression the reduced folate carrier (RFC). Transfection cells with RFC cDNA restored increased sensitivity by ∼50-fold. A CpG island promoter region of<i>RFC</i> found to be methylated but unmethylated expressing, methotrexate-sensitive MCF-7 cells. Chromatin immunoprecipitation antibodies against acetylated histones H3 H4 showed that <i>RFC</i>...