A5084276968- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Bioenergy crop production and management
- Pluripotent Stem Cells Research
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Information Systems Theories and Implementation
- Plant Genetic and Mutation Studies
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Renal and related cancers
- Assisted Reproductive Technology and Twin Pregnancy
- Wheat and Barley Genetics and Pathology
- Tissue Engineering and Regenerative Medicine
- Software Engineering Techniques and Practices
- Plant tissue culture and regeneration
- Genetically Modified Organisms Research
- Seed Germination and Physiology
- Phytase and its Applications
- Food composition and properties
- Business Process Modeling and Analysis
- CRISPR and Genetic Engineering
- Innovations in Medical Education
University of Copenhagen
1998-2024
Aarhus University
1994-2015
Karolinska University Hospital
1997-2006
University of Georgia
2004
University of Guelph
2004
South Australian Research and Development Institute
2004
Scientific and Technological Research Council of Turkey
2004
Bursa Uludağ Üni̇versi̇tesi̇
2004
Danish Cattle Research Centre
1994-1997
Royal Agricultural University
1989-1997
Although cryopreservation of certain mammalian embryos is now a routine procedure, considerable differences efficiency exist depending on stage, species and origin (in vivo or in vitro produced). Factors that are suspected to cause most these the amount intracellular lipid droplets different microtubular structure leading chilling injury as well volume/surface ratio influencing penetration cryoprotectants. A new approach, Open Pulled Straw (OPS) method, which renders very high cooling...
The assembly, rearrangement and disassembly of the synaptonemal complex during stages from leptotene to anaphase I meiosis are described. assembly lateral component between sister chromatids chromosome causes undivided appearance in light microscope. During zygotene mediates homologous segments as well homoeologous chromosomes. Chromosome bivalent interlockings a regular feature pairing at zygotene. They resolved by breakage precise repair phase formation is succeeded rearrangements. In this...
Culture of mammalian zygotes individually and in small groups results lower developmental rates than culture large groups. Zona-free also have impaired potential current systems. This paper describes a new approach to resolve the problems, Well (WOW) system. Small wells (WOWs) were formed four-well dishes by melting bottom with heated steel rods. The WOWs then rinsed, filled medium, embryos placed into WOWs. To test value WOW system 3 x factorial experiment was performed. Bovine presumptive...
Nutrients destined for the developing cereal grain encounter several restricting barriers on their path towards final storage sites in grain. In order to identify transporters and chelating agents that may be involved transport deposition of zinc barley grain, expression profiles have been generated four different tissue types: transfer cells, aleurone layer, endosperm, embryo. Cells from these tissues were isolated with 'laser capture microdissection' technology extracted RNA was subjected...
The kinetics of the in vitro development early embryos from bovine zygotes derived and were compared, investigating effect serum during maturation fertilization (IVM-IVF) culture. Zygotes collected superovulated heifers or produced immature oocytes with without supplementation, cultured subsequently defined culture medium (SOFaaci) supplementation. Time-lapse images recorded every 0.5 h throughout period. More vivo- than vitro-derived developed to compact morula blastocyst stages (87% versus...
The influence of various in vitro procedures on embryo survival and the production normal offspring was investigated sheep. Zygotes produced from matured (IVM) fertilized (IVF) oocytes derived slaughtered Merino ewes were allocated to three culture treatments for 6.5 days. Two groups cultured absence or presence oviduct epithelial cells while third group vivo oviducts synchronized ewes. A fourth zygotes obtained superovulated also served as controls. After culture, IVM–IVF morulae...
A simple procedure is described for the mechanical isolation of protoplasts unfertilized and fertilized barley egg cells from dissected ovules. Viable were isolated ~75% Unfertilized did not divide, whereas almost all developed into microcalli. These degenerated when grown in medium only. When cocultivated with microspores undergoing microspore embryogenesis, embryo-like structures that gave rise to fully fertile plants. On average, 75% structures. Fully plants regenerated ~50% The...
Day 7 bovine somatic nuclear transfer (NT) embryos reconstructed from granulosa cells were examined for numerical chromosome aberrations as a potential cause of the high embryonic and fetal loss observed in such after transfer. The NT using zona-free manipulation method: half-cytoplasts made oocytes by bisection, which two half-oocytes one cell (serum-starved primary culture) fused together activated. cultured modified synthetic oviductal fluid containing essential nonessential amino acids,...
Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods micromanipulation. Here, we present details development a technique for somatic cells that is both simpler faster than traditional methods. The comprises bisection zona-free oocytes reconstruction embryos comprising two half cytoplasts cell by adherence using phytohaemagglutinin-P (PHA) followed an electropulse subsequent culture in microwells (termed WOWs - well well). system was...
This paper discusses a rigorous relativistic treatment of the differential cross section for Compton scattering from bound electron states in impulse approximation. Starting with general central-field Hartree-Fock wave functions, simple expression is found which valid all completely occupied shells. then compared more approximate developed by R. Ribberfors [Phys. Rev. B 12, 2067 (1975)]. The profile well-defined concept nonrelativistic theory, but it not categorically applicable theory....