A5068789371- Atherosclerosis and Cardiovascular Diseases
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Nuclear Receptors and Signaling
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Cardiovascular Disease and Adiposity
- Circular RNAs in diseases
- Cytokine Signaling Pathways and Interactions
- Computational Drug Discovery Methods
- Cell Adhesion Molecules Research
- Global Socioeconomic and Political Dynamics
- Kruppel-like factors research
- Ferroptosis and cancer prognosis
- Antioxidant Activity and Oxidative Stress
- 3D Printing in Biomedical Research
- Chromosomal and Genetic Variations
- Vascular Anomalies and Treatments
- Social and Behavioral Studies
- Infrared Thermography in Medicine
- Bioinformatics and Genomic Networks
- vaccines and immunoinformatics approaches
- CRISPR and Genetic Engineering
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Genetics and Reproduction
Aarhus University
2022-2024
Research Institute of Medical Genetics of Russian Academy of Medical Sciences
2020-2021
Tomsk National Research Medical Center
2020-2021
Russian Academy of Sciences
2020
Siberian State Medical University
2018-2019
Research Institute of Complex Problems of Cardiovascular Disease Russian Academy of Medical Sciences
2019
Background and Aims: Proliferation of arterial smooth muscle cells (SMCs) their modulation to alternative mesenchymal phenotypes is a central mechanism in the growth atherosclerotic lesions. The underlying processes have been studied extensively mouse models, but detailed analysis when where modulated SMCs accumulate human atherosclerosis lacking. present study mapped SMC subtypes during progression coronary explored associations with disease carotid plaques. Methods: Multiplex...
In this study we performed a comparative gene expression analysis of carotid arteries in the area atherosclerotic plaques and healthy internal mammary patients with advanced atherosclerosis by using microarray HumanHT-12 BeadChip ("Illumina"). The most down-regulated genes were APOD, FABP4, CIDEC FOSB, up-regulated was SPP1 (|FC|>64; pFDR<0.05). majority differentially expressed plaques. Unexpectedly, involved immune inflammatory responses to compare (arachidonic acid metabolism,...
Abstract Recent genome-wide association studies (GWAS) have identified multiple vascular cell-expressed genes linked to coronary artery disease (CAD), suggesting that smooth muscle cells (SMCs) and SMC-derived metaplastic are promising targets for novel antiatherosclerosis therapies. However, the disease-promoting pathways of most GWAS-identified unknown, hindering their translation into therapeutic targets. This study integrated public GWAS data CAD single-cell RNA sequencing (scRNA-seq)...
The methylation index of the LINE-1 promoter is one most commonly used markers for assessing global level genome in various human cells and tissues. We developed an NGS-based protocol DNA analysis retrotransposon promoter. This approach allows assessment 19 CpG sites that have highest tissue- or tumor-specific variability. method provides a profile analyzing either each site independently mean across results obtained using corresponded well to assessed commercially available kit...
В работе проведен анализ уровня метилирования ретротранспозона LINE-1 в атеросклеротических бляшках сонных артерий, выделенных из них макрофагах и гладкомышечных клетках, а также лейкоцитах периферической крови пациентов с клинически выраженным атеросклерозом здоровых доноров. Выявлен сниженный уровень по сравнению лейкоцитами пациентов, его связь гистологическими признаками нестабильности атеросклеротической бляшки. We analyzed the methylation level in carotid atherosclerotic plaques,...
Aim. Comparative analysis of the deoxyribonucleic acid (DNA) methylation level in enhancer region CDKN2A/2B and CDKN2B-AS1 genes (9p21.3 locus) vessels with/without atherosclerotic lesions, as well leukocytes patients with clinically relevant carotid artery (CA) atherosclerosis healthy individuals. Material methods. The group included 22 individuals severe stenosis (>80%) CA. Samples plaques, presenting CA regions, great saphenous veins, peripheral blood samples (leukocytes) were obtained...
Abstract Objective Here, we identify and quantify leukocytes, macrophages, endothelial cells (ECs), vascular smooth muscle (VSMCs) with contractile macrophage-like phenotypes by flow cytometry to compare human early advanced coronary atherosclerotic plaques. Approach Results Sixteen lesions of 6 patients (3 women, 3 men, age 82 ± 9 years), including one case restenosis after stenting, were collected at autopsy. The cause death all was acute myocardial infarction. categorized into (EALs, n =...
Preparation of cell suspension from arterial walls is a key issue in planning the research aimed at studying role individual types human arteries vivo, both normal and affected with atherosclerosis. To date, there range approaches for disaggregating tissues. In this review, we report previously published data techniques addition, an algorithm able optimizing disaggregation proposed taking into account emerging problems probable solutions.
В работе проведен анализ профилей метилирования гена miR-10b и транскрипционного фактора TWIST1, регулирующего экспрессию данной микроРНК, в кровеносных сосудах лейкоцитах крови пациентов с клинически выраженным атеросклерозом у относительно здоровых лиц. Обнаружено гипометилирование MIR10B поражённых артериях непораженных артерий вен. Профиль TWIST1 был тканеспецифичен постоянен вне зависимости от наличия заболевания. We analyzed DNA methylation profiles within genes of and regulating the...