A5008214087- Atherosclerosis and Cardiovascular Diseases
- Carcinogens and Genotoxicity Assessment
- Chronic Lymphocytic Leukemia Research
- Advanced Fluorescence Microscopy Techniques
- Environmental Toxicology and Ecotoxicology
- Synthesis and pharmacology of benzodiazepine derivatives
- Toxic Organic Pollutants Impact
- Molecular Biology Techniques and Applications
- Boron Compounds in Chemistry
- Cellular Mechanics and Interactions
- Lymphoma Diagnosis and Treatment
- Synthesis and bioactivity of alkaloids
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Immunodeficiency and Autoimmune Disorders
- Cardiomyopathy and Myosin Studies
- Coronary Interventions and Diagnostics
- Pharmaceutical and Antibiotic Environmental Impacts
- Autophagy in Disease and Therapy
- Cell Image Analysis Techniques
- Advanced Control Systems Optimization
- Neurogenesis and neuroplasticity mechanisms
- Radiation Therapy and Dosimetry
- Advanced biosensing and bioanalysis techniques
- bioluminescence and chemiluminescence research
Spanish National Centre for Cardiovascular Research
2021-2024
Universidad Autónoma de Madrid
2004-2014
Consejo Superior de Investigaciones Científicas
2014
Centro de Biología Molecular Severo Ochoa
2011
To cause atherosclerosis, LDLs (low-density lipoproteins) must first pass through the endothelium and then become retained in arterial matrix. Which of these two processes is rate-limiting predicts topography plaque formation remains controversial. investigate this issue, we performed high-resolution mapping LDL entry retention murine aortic arches before during atherosclerosis development.Maps were created by injecting fluorescently labeled followed near-infrared scanning whole-mount...
Chronic lymphocytic leukemia (CLL) is an incurable disease characterized by intense trafficking of the leukemic cells between peripheral blood and lymphoid tissues. It known that ability lymphocytes to recirculate strongly depends on their capability rapidly rearrange cytoskeleton adapt external cues; however, little about differences occurring CLL healthy B during these processes. To investigate this point, we applied a single-cell optical (super resolution microscopy) nanomechanical...
ABSTRACT Myosin inhibitor mavacamten is the only targeted treatment available for hypertrophic cardiomyopathy (HCM), a disease caused by hundreds of genetic variants that affect mainly sarcomeric myosin and its negative regulator cardiac myosin-binding protein C (cMyBP-C, encoded MYBPC3 ). Here, we have examined whether reported limited efficacy in fraction HCM patients can result from dissimilar pathomechanisms triggered different variants, scenario particularly relevant -associated HCM. To...
Abstract In the present work, we have continued our studies on harmine phototoxicity in human tumour cells. The toxicity of dark was analysed by a quantitative neutral red uptake assay, and subcellular sensitive targets following photosensitization were defined electron microscopic analysis HeLa results obtained indicated that this compound shows clear dose‐dependent cytotoxic effect dark. combined treatment with suitable doses UV radiation very effective at an early stage, although maximal...
ABSTRACT Most studies addressing chromatin behaviour during preimplantation development are based on biochemical assays that lack spatial and cell-specific information, crucial early development. Here, we describe the changes in taking place at transition from totipotency to lineage specification, by using direct stochastical optical reconstruction microscopy (dSTORM) whole-mount embryos first stages of mouse Through study two post-translational modifications Histone 3 related active...
HS1, the hematopoietic homolog of cortactin, acts as a versatile actin-binding protein in leucocytes. After phosphorylation, it is involved GTPase and integrin activation, BCR, TCR, CXCR4 downstream signaling. In normal leukemic B cells, HS1 central cytoskeletal interactor its phosphorylation expression are prognostic factors chronic lymphocytic leukemia (CLL) patients. We here introduce for first time super-resolution imaging study based on single-cell 3D-STED microscopy optimized revealing...
Abstract During the last twenty years, interest in light microscopy and imaging techniques has grown various fields, such as molecular cellular biology, developmental neurobiology. In addition, number of scientific articles journals using these is rapidly increasing. Nowadays, most research institutions require sophisticated systems to cover their investigation demands. general, instruments are too expensive complex be purchased managed by a single laboratory or group, so they have shared...