A5062515479- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Blood properties and coagulation
- ATP Synthase and ATPases Research
- Erythrocyte Function and Pathophysiology
- 3D Printing in Biomedical Research
- Bat Biology and Ecology Studies
- Chronic Lymphocytic Leukemia Research
- Thermoregulation and physiological responses
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Monoclonal and Polyclonal Antibodies Research
- Physics and Engineering Research Articles
- Heat shock proteins research
- Plasma Applications and Diagnostics
- S100 Proteins and Annexins
- Microfluidic and Capillary Electrophoresis Applications
Universität Greifswald
2020-2024
Universitätsmedizin Greifswald
2020-2023
German Centre for Cardiovascular Research
2020-2023
Innovationszentrum für Mobilität und Gesellschaftlichen Wandel (Germany)
2020
Abstract In life sciences, the material properties of suspended cells have attained significance close to that fluorescent markers but with advantage label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded application mechanical assays fast on-the-fly phenotyping large sizes, has been restricted...
Metastatic melanoma is an aggressive and deadly disease. Therapeutic advance has been achieved by antitumor chemo‐ radiotherapy. These modalities involve the generation of reactive oxygen nitrogen species, affecting cellular viability, migration, immunogenicity. Such species are also created cold physical plasma, ionized gas capable redox modulating cells tissues without thermal damage. Cold plasma suggested for anticancer therapy. Here, cell toxicity, motility, immunogenicity murine...
Abstract Microfluidics by soft lithography has proven to be of key importance for biophysics and life science research. While being based on replicating structures a master mold using benchtop devices, design modifications are time consuming require sophisticated cleanroom equipment. Here, we introduce virtual fluidic channels as flexible robust alternative microfluidic devices made lithography. Virtual liquid-bound systems that can created in glass cuvettes tailored three dimensions within...
Jedes Jahr verschlafen Millionen von Tieren die kalten Wintermonate – doch wie schafft es ihr Körper, bei niedrigen Temperaturen weiterhin zu funktionieren? Wir haben nun mechanischen Eigenschaften roten Blutzellen Menschen sowie zwei Fledermausarten untersucht, denen eine Winterschlaf hält, während andere ganzjährig aktiv ist. Die Erkenntnisse könnten nicht nur unser Verständnis des natürlichen Winterschlafes vertiefen, sondern auch neue Möglichkeiten in Medizin und Raumfahrt eröffnen.
Chronic lymphocytic leukemia (CLL) is an incurable disease characterized by intense trafficking of the leukemic cells between peripheral blood and lymphoid tissues. It known that ability lymphocytes to recirculate strongly depends on their capability rapidly rearrange cytoskeleton adapt external cues; however, little about differences occurring CLL healthy B during these processes. To investigate this point, we applied a single-cell optical (super resolution microscopy) nanomechanical...
Real-time deformability cytometry is a unique tool for quality assessment of therapeutic blood cells utilizing their mechanical properties.
Hibernation is a widespread and highly efficient mechanism to save energy in mammals. However, one major challenge of hibernation maintaining blood circulation at low body temperatures, which strongly depends on the viscoelastic properties red cells (RBCs). Here, we examined physiologically relevant timescales thermomechanical hundreds thousands individual RBCs from hibernating common noctule bat ( Nyctalus noctula ), nonhibernating Egyptian fruit Rousettus aegyptiacus humans Homo sapiens )....
Mechanical properties have been proven to be a pivotal parameter enhance our understanding of living systems. While research during the last decades focused on cells and tissues, little is known about role organelle mechanics in cell function. Here, mitochondria are specific interest due their involvement numerous physiological pathological processes, e.g., production homeostasis reactive oxygen species (ROS). Using real-time fluorescence deformability cytometry, we present microfluidic...
Abstract Reactive oxygen species (ROS) are important secondary messengers involved in a variety of cellular processes, including activation, proliferation, and differentiation. Hydrogen peroxide (H 2 O ) is major ROS typically kept low nanomolar range that causes cell tissue damage at supraphysiological concentrations. While have been studied detail molecular scale, little known about their impact on mechanical properties as label-free biomarker for stress response. Here, we exposed human...
The capability to parameterize shapes is of essential importance in biomechanics identify cells, track their motion, and quantify deformation. While various shape descriptors have already been investigated study the morphology migration adherent little known how mathematical definition a contour impacts outcome rheological experiments on cells suspension. In microfluidic systems, hydrodynamic stress distributions induce time-dependent cell deformation that needs be quantified determine...