A5091772222- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Cell Image Analysis Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Digital Holography and Microscopy
- Blood properties and coagulation
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Fluid Dynamics and Thin Films
- Surface Modification and Superhydrophobicity
- Advanced Sensor and Energy Harvesting Materials
- Biosensors and Analytical Detection
- Lattice Boltzmann Simulation Studies
- Force Microscopy Techniques and Applications
- Nanofabrication and Lithography Techniques
- Optical measurement and interference techniques
- Microtubule and mitosis dynamics
- Electrowetting and Microfluidic Technologies
- Nanomaterials and Printing Technologies
- Optical Imaging and Spectroscopy Techniques
- Fluid Dynamics and Turbulent Flows
- Venomous Animal Envenomation and Studies
- Rheology and Fluid Dynamics Studies
Max Planck Institute for the Science of Light
2019-2024
Technische Universität Dresden
2015-2020
Center for Systems Biology Dresden
2019
Biotechnology Research Center
2018
Istituto Nanoscienze
2009-2014
University of Salento
2007-2013
Italian Institute of Technology
2008-2010
Center for Biomolecular Nanotechnologies
2010
Istituto Nazionale per la Fisica della Materia
2008-2009
Istituto Nazionale di Fisica Nucleare, Sezione di Lecce
2008
Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications biology medicine. A new method, real-time deformability cytometry, probes cell at high throughput by exposing cells to shear flow microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. However, observed deformations the channel not only are determined stiffness, but also depend size relative size. Here, we disentangle mutual...
The measurement of cell stiffness is an important part biological research with diverse applications in biology, biotechnology and medicine. Real-time deformability cytometry (RT-DC) a new method to probe at high throughput by flushing cells through microfluidic channel where deformation provides indicator for (Otto et al. cytometry: on-the-fly 725 mechanical phenotyping. Nat. Methods 2015, 12, 199–202). Here, we propose full numerical model single flow quantitatively relate parameters....
Abstract In life sciences, the material properties of suspended cells have attained significance close to that fluorescent markers but with advantage label-free and unbiased sample characterization. Until recently, cell rheological measurements were either limited by acquisition throughput, excessive post processing, or low-throughput real-time analysis. Real-time deformability cytometry expanded application mechanical assays fast on-the-fly phenotyping large sizes, has been restricted...
Abstract The mechanical properties of cancer cells and their microenvironment contribute to breast progression. While mechanosensing has been extensively studied using 2D substrates, much less is known about it in a physiologically more relevant 3D context. Here demonstrated that tumor spheroids, growing polyethylene glycol‐heparin hydrogels, are sensitive environment stiffness. During spheroid growth, compressive stresses up 2 kPa build up, as quantitated elastic polymer beads stress...
Standardized polyacrylamide microgel beads as novel tools to calibrate experiments in biomechanics and measure stresses complex tissues.
We present a bio-inspired renal microdevice that resembles the in vivo structure of kidney proximal tubule. For first time, population tubular adult stem/progenitor cells (ARPCs) was embedded into microsystem to create bioengineered These have both multipotent differentiation abilities and an extraordinary capacity for injured cell regeneration. Therefore, ARPCs may be considered promising tool promoting regenerative processes treat acute chronic injury. Here were grown confluence exposed...
Quantitative measurements of physical parameters become increasingly important for understanding biological processes. Brillouin microscopy (BM) has recently emerged as one technique providing the 3D distribution viscoelastic properties inside samples − so far relying on implicit assumption that refractive index (RI) and density can be neglected. Here, we present a novel method (FOB microscopy) combining BM with optical diffraction tomography epifluorescence imaging explicitly measuring...
Abstract Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such powerful to vivo pathological diagnosis remains challenging. Multi-core fiber bundles (MCFs) enable ultra-thin probes for imaging, but current MCF techniques are limited amplitude modalities. We demonstrate computational lensless microendoscope that uses an bare perform with microscale lateral resolution nanoscale axial...
Numerous cell functions are accompanied by phenotypic changes in viscoelastic properties, and measuring them can help elucidate higher level cellular health disease. We present a high-throughput, simple low-cost microfluidic method for quantitatively the elastic (storage) viscous (loss) modulus of individual cells. Cells suspended high-viscosity fluid pumped with high pressure through 5.8 cm long 200 µm wide channel. The shear stress induces large, ear ellipsoidal deformations. In addition,...
Sorting cells is an essential primary step in many biological and clinical applications such as high-throughput drug screening, cancer research cell transplantation. Cell sorting based on their mechanical properties has long been considered a promising label-free biomarker that could revolutionize the isolation of from heterogeneous populations. Recent advances microfluidic image-based analysis combined with subsequent by on-chip actuators demonstrated possibility physical properties....
In this letter, we demonstrate an unexpected surface-acoustic-wave (SAW)-driven pumping effect in hydrophobic polydimethilsiloxane (PDMS)-lithium niobate (LiNbO3) microchannels. Atomization within the fluidic channel followed by SAW-assisted coalescence leads to liquid counterflow with respect SAW propagation direction. This physical mechanism is contrasted acoustic-streaming process driving isolated drop displacement on piezoelectric substrates. principle shown not be readily applicable...
Cells alter the path of light, a fact that leads to well‐known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures biophysical property causes these aberrations, refractive index (RI). ODT is complementary fluorescence imaging and does not require any markers. The present study introduces RI with optofluidic rotation (RAFTOR) suspended cells, facilitating segmentation 3D‐correlated data for quantitative interpretation nuclear RI. technique validated...
Artificial surfaces that exhibit unidirectional water spreading and superhydrophobicity are obtained by Strelitzia reginae leaves. Both green dried leaves used, thus exploiting the plant senescence. We demonstrate natural drying process of strongly affects surface morphology wettability. Polymeric stamps from leaf show an arrangement periodic microridges/microgrooves favor anisotropic wetting, with a contact angle (WCA) variation about 21% along two principal directions. Instead, shrinkage...
Mechanical stress exerted and experienced by cells during tissue morphogenesis organ formation plays an important role in embryonic development. While techniques to quantify mechanical stresses vitro are available, few methods exist for studying living organisms. Here, we describe characterize cell-like polyacrylamide (PAAm) bead sensors with well-defined elastic properties size vivo quantification of cell-scale stresses. The beads were injected into developing zebrafish embryos their...
We use hyperbolic microchannels to create a flow field with linearly increasing velocity, leading constant tensile stress probe the viscoelastic properties of cells. verify our approach on oil droplets and polyacrylamide beads.
We show how the capillary filling of microchannels is affected by posts or ridges on sides channels. Ridges perpendicular to flow direction introduce contact line pinning, which slows, sometimes prevents, filling, whereas parallel provide extra surface that may enhance filling. Patterning microchannel with square has little effect ability a channel fill for equilibrium angle ${\ensuremath{\theta}}_{e}\ensuremath{\lesssim}30\ifmmode^\circ\else\textdegree\fi{}$. For...
Significance Microtubules consist of parallel protofilament lanes for motor-based intracellular transport. While most motors translocate along individual protofilaments, members the kinesin-8 family have been reported to sidestep. However, mechanism sidestepping is currently not understood. Here, we track 3D motion single on freely suspended microtubules. We find that sidestep with a bias left, probability which increased time taken per forward step. relate this behavior bifurcation in step...
This paper presents prototypical microfluidic devices made by hybrid microchannels based on piezoelectric LiNbO3 and polydimethylsiloxane. system enables withdrawing micropumping acoustic radiation in microchannels. The configuration, integrated chip, is here quantitatively investigated for the first time, found to be related formation coalescence dynamics of droplets within microchannel, primed surface waves. growth governed water diffusion LiNbO3, determining advancement fluid front....
Fully controlled liquid injection and flow in hydrophobic polydimethylsiloxane (PDMS) two-dimensional microchannel arrays based on on-chip integrated, low-voltage-driven micropumps are demonstrated. Our architecture exploits the surface-acoustic-wave (SAW) induced counterflow mechanism effect of nebulization anisotropies at crossing areas owing to lateral propagating SAWs. We show that by selectively exciting single or multiple SAWs, fluids can be drawn from their reservoirs moved towards...
Abstract Here we show an innovative, simple and reliable method to fabricate micro‐lasers by self‐assembly of rod‐shaped nanocrystals. We use dot/rod core/shell CdSe/CdS nanorods form optical micro‐resonators exploiting their self‐organization into well‐defined coffee stain rings. The fabrication process merely consists capillary jet deposition a nanorod solution onto glass substrate, is scalable, economic, highly reproducible. Upon pumping the obtain laser emission in red or blue‐green...
Neuromuscular circuits (NMCs) are vital for voluntary movement, and effective models of NMCs needed to understand the pathogenesis of, as well identify treatments for, multiple diseases, including Duchenne's muscular dystrophy amyotrophic lateral sclerosis. Microfluidics ideal recapitulating central peripheral compartments NMCs, but myotubes often detach before functional formed. In addition, microfluidic systems limited a single experimental unit, which significantly limits their...