A5032572916- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Receptor Mechanisms and Signaling
- Cellular transport and secretion
- Neuropeptides and Animal Physiology
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Neuroendocrine regulation and behavior
- Cardiac electrophysiology and arrhythmias
- Hypothalamic control of reproductive hormones
- Stress Responses and Cortisol
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Axon Guidance and Neuronal Signaling
- Histone Deacetylase Inhibitors Research
- Regulation of Appetite and Obesity
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Endoplasmic Reticulum Stress and Disease
- Cellular Mechanics and Interactions
- Advanced Proteomics Techniques and Applications
- Cell Image Analysis Techniques
- Sirtuins and Resveratrol in Medicine
- Signaling Pathways in Disease
Centro de Biología Molecular Severo Ochoa
2008-2025
Universidad Autónoma de Madrid
2009-2025
Consejo Superior de Investigaciones Científicas
1996-2016
In-Q-Tel
2010
Biotechnology and Biological Sciences Research Council
1989
Babraham Institute
1986-1988
Institute of Animal Physiology of the Slovak Academy of Sciences
1988
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
1988
Rationale: In cardiomyocytes, Na V 1.5 and Kir2.1 channels interact dynamically as part of membrane bound macromolecular complexes. Objective: The objective this study was to test whether preassemble during early forward trafficking travel together common microdomains. Methods Results: patch-clamp experiments, coexpression trafficking-deficient mutants Δ314-315 or R44A/R46A with wild-type (WT) WT in heterologous cells reduced inward sodium current compared alone coexpressed . cell surface...
Cardiac Nav1.5 and Kir2.1-2.3 channels generate Na (INa) inward rectifier K (IK1) currents, respectively. The functional INa IK1 interplay is reinforced by the positive reciprocal modulation between Nav15 Kir2.1/2.2 to strengthen control of ventricular excitability. Loss-of-function mutations in SCN5A gene, which encodes channels, underlie several inherited arrhythmogenic syndromes, including Brugada syndrome (BrS). We investigated whether presence BrS-associated alters density concomitantly...
Cardiac Kir2.1 and Nav1.5 channels generate the inward rectifier K+ (IK1) Na+ (INa) currents, respectively. There is a mutual interplay between ventricular INa IK1 densities, because exhibit positive reciprocal modulation. Here we compared some of biological properties when they are expressed together or separately to get further insights regarding their putative interaction. First demonstrated by proximity ligation assays that in membrane myocytes proteins close each other (<40 nm apart)....
CULTURES of hippocampal neurones have been used to study a possible correlation between the pattern dendritic growth and post-translational modification Microtubule-Associated Protein 2 (MAP2). During first three days in vitro, small increase total amount MAP2 is observed, whereas level phosphorylation increases exponentially, being particularly dramatic after vitro. Analysis dendrite morphology by immunofluorescence, revealed parallel exponential arborization with maximum rates at third day...
The GLYT1 (glycine transporter-1) regulates both glycinergic and glutamatergic neurotransmission by controlling the reuptake of glycine at synapses. Trafficking to cell surface is critical for its function. In present paper, using mutational analysis C-terminal domain, we identified evolutionarily conserved motif R575L576(X8)D585 as being necessary ER (endoplasmic reticulum) export. This probably due capacity bind Sec24D, a component COPII (coatomer coat protein II) complex. export was...
Neurogranin (Ng) is a 78-amino-acid-long protein concentrated at dendritic spines of forebrain neurons that involved in synaptic plasticity through the regulation CaM (calmodulin)-mediated signalling. Ng features central IQ motif mediates binding to and phosphorylated by PKC (protein kinase C). We have analysed subcellular distribution found it associates cellular membranes rat brain. In vitro assays revealed selectively binds PA (phosphatidic acid) this interaction prevented...
Hippocampal neurones, from embryonic rats, were cultured for different times and the extension of dendrite-like processes was analysed morphologically by immunofluorescence, using microtubule-associated protein 2 (MAP2) as a marker. Simultaneously, changes in phosphorylation MAP2 analyzed correlation between dendrite sprouting an increase found. Phospho-MAP2 cleaved Staphylococcus aureus V8 protease limited proteolysis its phosphopeptide pattern compared to that obtained with two kinases...
We developed genetically-encoded fluorescent sensors based on Förster Resonance Energy Transfer to monitor phosphatidic acid (PA) fluctuations in the plasma membrane using Spo20 as PA-binding motif. Basal PA levels and phospholipase D activity varied different cell types. In addition, stimuli that activate phosphatases, leading lower levels, increased lamellipodia filopodia formation. Lower were observed edge than trailing of migrating HeLa cells. MSC80 OLN93 cells, which are stable lines...
Abstract A panel of four anti‐MAP1B antibodies have been used to study the presence and post‐translational modification MAP1B in primary cultures glial cells. Two (150 125) recognize phosphorylated epitopes whereas other two (531 842) non‐phosphorylated phosphorylatable on molecule. Immunofluorescence Western blot analysis with 531 842 revealed small amounts MAP1B‐like immunoreactivity type 1 astrocytes a greater content more differentiated cells found long‐term cultures. By...
Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages differentiation. Our findings reveal the existence two distinct patterns expression. Firstly, conventional PKC α, β γ, that are expressed very low levels during initial then increase continuously with time culture. Secondly, novel δ, ϵ ζ, whose contents early to reach a maximum after three days culture progressively decline. Specific proteolysis...