A5014261602- Neuroinflammation and Neurodegeneration Mechanisms
- Adenosine and Purinergic Signaling
- Vasculitis and related conditions
- Immune Response and Inflammation
- Immune cells in cancer
- Extracellular vesicles in disease
- Acute Ischemic Stroke Management
- Immune Cell Function and Interaction
- Calcium signaling and nucleotide metabolism
- Barrier Structure and Function Studies
- Escherichia coli research studies
- S100 Proteins and Annexins
- Complement system in diseases
- Adolescent and Pediatric Healthcare
- Multiple Sclerosis Research Studies
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Atherosclerosis and Cardiovascular Diseases
- Intracerebral and Subarachnoid Hemorrhage Research
- Nitric Oxide and Endothelin Effects
- Neurological Complications and Syndromes
- Neurological Disease Mechanisms and Treatments
- Characterization and Applications of Magnetic Nanoparticles
- Sarcoidosis and Beryllium Toxicity Research
- Otitis Media and Relapsing Polychondritis
Universität Hamburg
2015-2024
University Medical Center Hamburg-Eppendorf
2015-2024
Klinik und Poliklinik für Neurologie
2012-2022
Ikerbasque
2022
University of the Basque Country
2022
Achucarro Basque Center for Neuroscience
2022
Center for Neurosciences
2022
Ludwig-Maximilians-Universität München
2021
University Hospital Schleswig-Holstein
2019
University of Lübeck
2019
Ischemic stroke leads to significant morbidity and mortality in the Western world. Early reperfusion strategies remain treatment of choice but can initiate augment an inflammatory response causing secondary brain damage. The understanding postischemic inflammation is very limited. objectives this study were define temporal spatial infiltration immune cell populations their activation patterns a murine cerebral ischemia-reperfusion injury model.Transient middle artery occlusion was induced...
Multi-protein complexes called inflammasomes have recently been identified and shown to contribute cell death in tissue injury. Intravenous immunoglobulin (IVIg) is an FDA-approved therapeutic modality used for various inflammatory diseases. The objective of this study investigate dynamic responses the NLRP1 NLRP3 stroke determine whether can be targeted with IVIg intervention. Primary cortical neurons were subjected glucose deprivation (GD), oxygen–glucose (OGD) or simulated...
The fast and accurate assessment of cerebral perfusion is fundamental for the diagnosis successful treatment stroke patients. Magnetic particle imaging (MPI) a new radiation-free tomographic method with superior temporal resolution, compared to other conventional methods. In addition, MPI scanners can be built as prehospital mobile devices, which require less complex infrastructure than computed tomography (CT) magnetic resonance (MRI). With these advantages, could accelerate treatment,...
Microglia survey the brain microenvironment for signals of injury or infection and are essential initiation resolution pathogen- tissue damage-induced inflammation. Understanding mechanism microglia responses during pathology is hence vital to promote regenerative responses. Here, we analyzed role purinergic receptor P2X4 (P2X4R) in microglia/macrophages autoimmune Blockade P2X4R signaling exacerbated clinical signs experimental encephalomyelitis (EAE) model also favored activation a...
The P2X7 channel is involved in the pathogenesis of various CNS diseases. An increasing number studies suggest its presence neurons where putative functions remain controversial for more than a decade. To resolve this issue and to provide model analysis functions, we generated BAC transgenic mice that allow visualization functional EGFP-tagged receptors vivo. Extensive characterization these revealed dominant P2X7-EGFP protein expression microglia, Bergmann glia, oligodendrocytes, but not...
Abstract In multiple sclerosis and the experimental autoimmune encephalomyelitis (EAE) model, both resident microglia infiltrating macrophages contribute to demyelination as well spontaneous remyelination. Nevertheless, specific roles of versus are unknown. We investigated influence in EAE using colony stimulating factor 1 receptor (CSF-1R) inhibitor, PLX5622, deplete microglial population Ccr2 RFP/+ fms EGFP/+ mice, distinguish blood-derived from microglia. PLX5622 treatment depleted...
Abstract Predicting the rate of disease progression has become important as trials new medical treatments for amyotrophic lateral sclerosis (ALS) are planned. Bulbar onset, early impairment forced vital capacity, and older age have all been associated with shorter survival. We performed a retrospective study to compare survival factors in German ALS population. analyzed 155 patients at intervals 4 months over period 3 years. To evaluate progression, functional rating scale (ALS‐FRS),...
<h3>Background</h3> Patients with encephalitis associated antibodies against N-methyl-D-aspartate-receptor antibody (NMDAR-ab) frequently show psychotic symptoms, amnesia, seizures and movement disorders. While brain MRI in NMDAR-ab is often normal, abnormalities of cerebral glucose metabolism have been demonstrated by positron emission tomography (PET) 18F-fluorodeoxyglucose(FDG) a few usually isolated case reports. However, common pattern FDG-PET has not reported. <h3>Methods</h3> The...
Relapsing symptoms post herpes simplex virus-1 (HSV) encephalitis (HSVE) usually occur a few weeks after viral therapy and represent either 1) true relapse of HSVE (CSF PCR positive for HSV, new necrotic lesions on brain MRI, response to acyclovir therapy) or 2) disorder postulated be immune-mediated negative no lesions, acyclovir).1,2 It has been suggested that this may related NMDA receptor (NMDAR) antibodies,3 we recently reported child in whom relapsing were the presentation anti-NMDAR...
Ion channels are desirable therapeutic targets, yet ion channel-directed drugs with high selectivity and few side effects still needed. Unlike small-molecule inhibitors, antibodies highly selective for target antigens but mostly fail to antagonize channel functions. Nanobodies-small, single-domain antibody fragments-may overcome these problems. P2X7 is a ligand-gated that, upon sensing adenosine 5'-triphosphate released by damaged cells, initiates proinflammatory signaling cascade, including...
Indoxyl sulfate (IS) is a protein-bound uremic toxin resulting from the metabolism of dietary tryptophan which accumulates in patients with impaired renal function, such as chronic kidney disease (CKD). IS well-known nephrovascular but little known about its effects on central nervous system (CNS) cells. Considering growing interest field CNS comorbidities CKD, we studied effect (15-60 μM) treatment C6 astrocyte cells increased reactive oxygen species release and decreased nuclear factor...
Background— Endogenous arginine homologues, including homoarginine, have been identified as novel biomarkers for cardiovascular disease and outcomes. Our studies of human cohorts a confirmatory murine model associated the homologue homoarginine its metabolism with stroke pathology outcome. Methods Results— Increasing levels were independently reduction in all-cause mortality patients ischemic (7.4 years follow-up; hazard ratio 1-SD 0.79 [95% confidence interval, 0.64–0.96]; P =0.019; n=389)....
The aim of this study was to describe the neurological syndrome in largest cohort adult patients with a complicated Shiga toxin-producing Escherichia coli infection. recent outbreak E. serotype O104:H4 northern Germany affected more than 3842 patients, 22% whom developed haemolytic uraemic syndrome. proportion unusually high, and complications were frequent severe. In three hospitals, population-based evaluation 217 infection carried out, including neurological, neuroradiological,...
The development of the brain tissue damage in ischemic stroke is composed an immediate component followed by inflammatory response with secondary after reperfusion. Fisetin, a flavonoid, has multiple biological effects, including neuroprotective and antiinflammatory properties. We analyzed effects fisetin on infarct size mouse model stroke, temporary middle cerebral artery occlusion, activation immune cells, murine primary N9 microglial Raw264.7 macrophage cells human macrophages, vitro cell...
Abstract Immune cells at sites of inflammation are continuously activated by local antigens and cytokines, regulatory mechanisms must be enacted to control inflammation. The stepwise hydrolysis extracellular ATP ectonucleotidases CD39 CD73 generates adenosine, a potent immune suppressor. Here we report that human effector CD8 T contribute adenosine production releasing CD73-containing vesicles upon activation. These have AMPase activity, the resulting mediates suppression independently...