A5069449607- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Disease Mechanisms and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Autophagy in Disease and Therapy
- Acute Ischemic Stroke Management
- Metabolism, Diabetes, and Cancer
- Complement system in diseases
- Cardiac Ischemia and Reperfusion
- Barrier Structure and Function Studies
- Regulation of Appetite and Obesity
- Inflammasome and immune disorders
- Thermal Regulation in Medicine
- S100 Proteins and Annexins
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Cholesterol and Lipid Metabolism
- NF-κB Signaling Pathways
- Protease and Inhibitor Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Animal Virus Infections Studies
- Endoplasmic Reticulum Stress and Disease
- Neurological Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Biomarkers in Disease Mechanisms
- Bacillus and Francisella bacterial research
National Neuroscience Institute
2014-2022
Abbott (Singapore)
2022
The University of Queensland
2009-2013
Texas Tech University
2008-2009
Texas Tech University Health Sciences Center
2008-2009
Flinders University
2008
Hunter Medical Research Institute
2008
Gosford Hospital
2008
University of Newcastle Australia
2008
The Royal Melbourne Hospital
2008
Stroke is the world's second leading cause of mortality, with a high incidence severe morbidity in surviving victims. There are currently relatively few treatment options available to minimize tissue death following stroke. As such, there pressing need explore, at molecular, cellular, tissue, and whole body level, mechanisms damage CNS an ischemic brain event. This review explores etiology pathogenesis stroke, provides general model such. The pathophysiology cerebral injury explained,...
Multi-protein complexes called inflammasomes have recently been identified and shown to contribute cell death in tissue injury. Intravenous immunoglobulin (IVIg) is an FDA-approved therapeutic modality used for various inflammatory diseases. The objective of this study investigate dynamic responses the NLRP1 NLRP3 stroke determine whether can be targeted with IVIg intervention. Primary cortical neurons were subjected glucose deprivation (GD), oxygen–glucose (OGD) or simulated...
The development of the brain tissue damage in ischemic stroke is composed an immediate component followed by inflammatory response with secondary after reperfusion. Fisetin, a flavonoid, has multiple biological effects, including neuroprotective and antiinflammatory properties. We analyzed effects fisetin on infarct size mouse model stroke, temporary middle cerebral artery occlusion, activation immune cells, murine primary N9 microglial Raw264.7 macrophage cells human macrophages, vitro cell...
C5a receptors are found in the central nervous system (CNS), on both neurons and glia. However, origin of C5a, which activates these receptors, is unclear. In present study, we show that primary cultured mouse cortical constitutively express C5, precursor classical receptor for CD88. With cell ischemia caused by 12 h glucose deprivation, or oxygen-glucose deprivation (OGD), demonstrated increased apoptosis, up-regulation CD88, levels media. Exogenous murine (100 nM) added to neuronal...
Notch-1 (Notch) is a cell surface receptor that regulates cell-fate decisions in the developing nervous system, and it may also have roles synaptic plasticity adult brain. Binding of its ligands results proteolytic cleavage Notch by γ-secretase enzyme complex, thereby causing release intracellular domain (NICD) translocates to nucleus, which transcription. Here we show activation modulates ischemic neuronal death vitro vivo. Specifically, our findings from use siRNA or overexpression NICD...
Ephrin (Eph) signaling within the central nervous system is known to modulate axon guidance, synaptic plasticity, and promote long-term potentiation. We investigated potential involvement of EphA2 receptors in ischemic stroke-induced brain inflammation a mouse model focal stroke. Cerebral ischemia was induced male C57Bl6/J wild-type (WT) EphA2-deficient (EphA2−/−) mice by middle cerebral artery occlusion (MCAO; 60 min), followed reperfusion (24 or 72 h). Brain infarction measured using...
Adiponectin is a hormone produced in and released from adipose cells, which has been shown to have anti-diabetic anti-inflammatory actions peripheral cells. Two cell surface adiponectin receptors (ADRs) mediate the majority of known biological adiponectin. Thus far, ADR expression brain demonstrated arcuate paraventricular nucleus hypothalamus, where its activation affects food intake. Recent findings suggest that levels circulating increase after an ischemic stroke, but role receptor stroke...
Intravenous immunoglobulin (IVIg) preparations obtained by fractionating blood plasma, are increasingly being used as an effective therapeutic agent in treatment of several inflammatory diseases. Its use a potential for stroke and Alzheimer's disease has been proposed, but little is known about the neuroprotective mechanisms IVIg. In this study, we investigated effect IVIg on downstream signaling pathways that involved neuronal cell death experimental models disease. Treatment cultured...
Intestinal ischemia-reperfusion (I/R) injury is a well-established animal model of systemic inflammation and can lead to multiple organ failure as well severe lasting morbidity even death. It occur in humans result vascular surgery or secondary sequelae many common conditions including low blood pressure, myocardial infarction, necrotizing enterocolitis. Systemic induced through kidney I/R has been shown previously encephalopathic adverse effects, it was theorized that intestinal would also...
Emerging studies implicate energy dysregulation as an underlying trigger for Parkinson's disease (PD), suggesting that a better understanding of the molecular pathways governing homeostasis could help elucidate therapeutic targets disease. A critical cellular regulator is AMP kinase (AMPK), which we have previously shown to be protective in PD models. However, precisely how AMPK function impacts on dopaminergic neuronal survival and pathogenesis remains elusive. Here, showed Drosophila...
Parkinson's disease (PD) is a prevalent neurodegenerative movement disorder that characterized pathologically by the progressive loss of dopaminergic (DA) neurons in substantia nigra pars compacta (SNpc) midbrain. Despite intensive research, etiology PD remains poorly understood. Interestingly, recent studies have implicated neuronal energy dysregulation as one key perpetrators disease. Supporting this, we recently demonstrated pharmacological or genetic activation AMP kinase (AMPK), master...
Abnormal lipid homeostasis has been observed in the brain of Parkinson's disease (PD) patients and experimental models, although mechanism underlying this phenomenon is unclear. Notably, previous studies have reported that PD-linked protein Parkin functionally interacts with important regulators, including Sterol Regulatory Element-Binding Proteins (SREBPs) cluster differentiation 36 (CD36). Here, we demonstrate a functional relationship between lipoprotein lipase (LPL), triglyceride widely...
Background and Purpose The Down syndrome candidate region 1 (DSCR1) gene is located on human chromosome 21 its protein over-expressed in brains of individuals. DSCR1 can modulate the activity calcineurin, a phosphatase abundant brain, but influence stroke outcome not clear. We compared wildtype (WT) transgenic (DSCR1-TG) mice which over-express isoform DSCR1. Methods Transient cerebral ischemia was produced by occlusion middle artery for 0.5 h. After 23.5 h reperfusion, we assessed...
Introduction: We documented the total spike antibody (S-Ab), IgG S-Ab and neutralizing (N-Ab) responses of BNT162b2/CoronaVac vaccinees up to 90 days post-booster dose. Methods: included 32 homologous regimen CoronaVac 136 BNT162b2 mRNA vaccinees. tested their (Roche), (Abbott) N-Ab (Snibe) levels at set time points from January 2021 April 2022. All subjects were deemed be COVID-19-naïve either via clinical history (CoronaVac vaccinees) or nucleocapsid testing (BNT162b2 vaccinees). Results:...
with poor collaterals, p = 0.002).Correspondingly, most patients (11/16) penumbra<66% had collateral status, versus 5/20 penumbra > 66% (p 0.02).Of the 15 and good eight an excellent outcome (mRS 0-1) compared 0/5 collaterals 0.05).Ten of major reperfusion at 24-h a outcome, 2/21 without < 0.001).However, was predominantly beneficial in 9/10 1/5 0.008). Conclusion:Patients large have high probability clinical provided they also subsequent occurs.
with poor collaterals, p = 0.002).Correspondingly, most patients (11/16) penumbra<66% had collateral status, versus 5/20 penumbra > 66% (p 0.02).Of the 15 and good eight an excellent outcome (mRS 0-1) compared 0/5 collaterals 0.05).Ten of major reperfusion at 24-h a outcome, 2/21 without < 0.001).However, was predominantly beneficial in 9/10 1/5 0.008). Conclusion:Patients large have high probability clinical provided they also subsequent occurs.