A5001037158- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Olfactory and Sensory Function Studies
- Optical Imaging and Spectroscopy Techniques
- Cannabis and Cannabinoid Research
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Advanced Chemical Sensor Technologies
- Alzheimer's disease research and treatments
- Anesthesia and Neurotoxicity Research
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Photoacoustic and Ultrasonic Imaging
- Cancer Research and Treatments
- Optical Coherence Tomography Applications
- Genetic Neurodegenerative Diseases
- Ubiquitin and proteasome pathways
Centre Interdisciplinaire de Recherche en Biologie
2017-2024
Institut Interdisciplinaire de Neuroscience
2022-2024
Université de Bordeaux
2022-2024
Institute of Bioorganic Chemistry
2020-2023
Collège de France
2017-2022
Université Paris Sciences et Lettres
2019-2022
Centre National de la Recherche Scientifique
2017-2022
Inserm
2017-2022
RIKEN Center for Brain Science
2014-2022
Sorbonne Université
2018
Abstract Dysfunctions in brain cholesterol homeostasis have been extensively related to disorders. The main pathway for elimination is its hydroxylation into 24S-hydroxycholesterol by the 24-hydrolase, CYP46A1. Increasing evidence suggests that CYP46A1 has a role pathogenesis and progression of neurodegenerative disorders, increasing levels neuroprotective. However, mechanisms underlying this neuroprotection remain be fully understood. Huntington’s disease fatal autosomal dominant caused an...
Neural computational power is determined by neuroenergetics, but how and which energy substrates are allocated to various forms of memory engram unclear. To solve this question, we asked whether neuronal fueling glucose or lactate scales differently upon increasing neural computation cognitive loads. Here, using electrophysiology, two-photon imaging, tasks, mathematical modeling, show that both involved in formation, with supporting long-term synaptic plasticity evoked high-stimulation load...
Synaptic NMDA receptors (NMDARs) carry inward Ca(2+) current responsible for postsynaptic signaling and plasticity in dendritic spines. Whether the concurrent K(+) efflux through same into synaptic cleft has a physiological role is not known. Here, we report that NMDAR-dependent can provide retrograde signal synapse. In hippocampal CA3-CA1 synapses, bulk of astrocytic triggered by activity reflected local NMDARs. The extracellular rise produced activation NMDARs boosted action...
Abstract Astrocytes express a complex repertoire of intracellular Ca 2+ transients (events) that represent major form signaling within individual cells and in astrocytic syncytium. These events have different spatiotemporal profiles, which are modulated by neuronal activity. Spontaneous appear more frequently distal processes independently from each other. However, little is known about the mechanisms underlying such subcellular distribution events. Here, we identify initiation points...
In the adult brain, GABA exerts either depolarizing or hyperpolarizing effects on neuronal membranes, depending neuron type, subcellular location, and activity. Depolarizing typically inhibits neurons through shunting, which is characterized by increased membrane conductance upon A receptor activation; however, it can also excite recruiting voltage-dependent conductances. The net influence of these opposing actions glutamatergic synaptic inputs remains incompletely understood. Here, we...
Glutamatergic transmission prompts K
The dorsal striatum exhibits bidirectional corticostriatal synaptic plasticity, NMDAR and endocannabinoids (eCB) mediated, necessary for the encoding of procedural learning. Therefore, characterizing factors controlling plasticity is crucial importance. Brain-derived neurotrophic factor (BDNF) its receptor, tropomyosine receptor kinase-B (TrkB), shape striatal functions, their dysfunction deeply affects basal ganglia. BDNF/TrkB signaling controls in various brain structures including...
Abstract Progress in neuroscience research hinges on technical advances visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require fixation (electron microscopy), do not have cellular resolution (magnetic resonance imaging) or only give a fragmented view (fluorescence microscopy). Here, we show how regular light microscopy together fluorescence labeling of the interstitial fluid extracellular space provide comprehensive optical...
Synaptic plasticity is triggered by different patterns of network activity. Here, we investigated how LTP in CA3-CA1 synapses induced stimulation affected tonic GABA A conductances rat hippocampal slices. Spike-timing-dependent was pairing Schaffer collateral with antidromic CA1 pyramidal neurons. Theta-burst-induced theta-burst collaterals. We mimicked increased conductance bath application 30 μ m GABA. Surprisingly, selectively suppressed theta-burst-induced but not spike-timing-dependent...
Abstract A miniature postsynaptic current (mPSC) is a small, rare, and highly variable spontaneous synaptic event that generally caused by the release of single vesicles. The amplitude variability mPSCs are key measures processes taken as main characteristics an elementary unit (quantal size) in traditional quantal analysis transmission. Due to different sources biological measurement noise, recordings exhibit high trial-to-trial heterogeneity, experimental measurements usually noisy scarce,...
The aim of the investigation was to study astroglia functional activity in hippocampal slices rat at different postnatal development stages by modeling metabolic changes brain.Materials and Methods.The carried out on Wistar rats three age groups: days 5-10, 10-20, more than 20 animal using a calcium imaging method.Results.In early developmental stages, normal astroglial found require additional energy substrates.Changing brain state low temperature had no significant effect astrocyte all...
Abstract Astrocytes express a complex repertoire of intracellular Ca 2+ transients (events) that represent major form signaling within individual cells and in the astrocytic syncytium. These events have different spatiotemporal profiles, which are modulated by neuronal activity. Spontaneous appear more frequently distal processes independently from each other. However, little is known about mechanisms underlying such subcellular distribution events. Here we identify initiation points...
<title>Abstract</title> Progress in neuroscience research hinges on technical advances visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require fixation, do not have cellular resolution or only give a fragmented view. Here, we show how regular light microscopy together fluorescence labeling of the interstitial fluid extracellular space provide comprehensive optical access real-time to anatomical complexity dynamics tissue.
The brain extracellular matrix is secreted by neurons and glial cells represents a molecular net comprised of polysaccharides proteins, fulfilling the space between in tissue.A complex structure ubiquitous localization underlie its involvement numerous important functions, such as diffusion regulation, cell-to-cell interactions, synaptic plasticity learning.Additionally, participates regeneration neuronal connections after spinal cord injuries.The ability to regenerate attenuates fast...
ABSTRACT The dorsal striatum exhibits bidirectional corticostriatal synaptic plasticity, NMDAR- and endocannabinoids-(eCB)-mediated, necessary for the encoding of procedural learning. Therefore, characterizing factors controlling plasticity is crucial importance. Brain-derived neurotrophic factor (BDNF) its receptor, tropomyosine receptor kinase-B (TrkB), shape striatal functions their dysfunction deeply affect basal ganglia. BDNF/TrkB signaling controls NMDAR-plasticity in various brain...
Abstract Progress in neuroscience research hinges on technical advances visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require fixation, do not have cellular resolution or only give a fragmented view. Here, we show how regular light microscopy together fluorescence labeling of the interstitial fluid extracellular space provide comprehensive optical access real-time to anatomical complexity dynamics tissue.
The aim of the study was to understand effect alterations in local ionic concentrations due neuronal activity on calcium astrocytes.Materials and Methods.In this we investigated astrocytic dynamics using confocal microscopy hippocampal slices from Wistar rats P15-18.Results.Here demonstrated that activation metabotropic glutamate receptors astrocytes led an increase frequency duration events.Elevations extracellular concentration did not change duration.Elevations potassium increased reduced...