A5050019198- Connexins and lens biology
- Mining Techniques and Economics
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Tunneling and Rock Mechanics
- Ion channel regulation and function
- Belt Conveyor Systems Engineering
- Infrastructure Maintenance and Monitoring
- Nicotinic Acetylcholine Receptors Study
- Transport Systems and Technology
- Asphalt Pavement Performance Evaluation
- Biochemical effects in animals
- Cannabis and Cannabinoid Research
- Adenosine and Purinergic Signaling
- American Constitutional Law and Politics
- Photoreceptor and optogenetics research
- Distributed and Parallel Computing Systems
- Underground infrastructure and sustainability
- Ion Channels and Receptors
- Occupational Health and Safety Research
- Colonialism, slavery, and trade
- Geotechnical and Geomechanical Engineering
- Transportation Safety and Impact Analysis
- Nitric Oxide and Endothelin Effects
- Sleep and Wakefulness Research
University of Calgary
2014-2024
Allen Institute for Brain Science
2011-2024
University of British Columbia
2006-2023
Université de Bordeaux
2022
Curtin University
2018
Alberta Bible College
2014
Calgary Laboratory Services
2011
University of Pretoria
1997-2008
Western University
2008
McMaster University
1997-2007
Neuronal excitotoxicity during stroke is caused by activation of unidentified large-conductance channels, leading to swelling and calcium dysregulation. We show that ischemic-like conditions [O 2 /glucose deprivation (OGD)] open hemichannels, or half gap junctions, in neurons. Hemichannel opening was indicated a large linear current flux across the membrane small fluorescent molecules. Single-channel openings hemichannels (530 picosiemens) were observed OGD. Both dye blocked inhibitors...
Pannexin-1 (Px1) is expressed at postsynaptic sites in pyramidal neurons, suggesting that these hemichannels contribute to dendritic signals associated with synaptic function. We found that, N-methyl-d-aspartate receptor (NMDAR) activation induced a secondary prolonged current and dye flux were blocked specific inhibitory peptide against Px1 hemichannels; knockdown of by RNA interference the cultured neurons. Enhancing endogenous NMDAR brain slices removing external magnesium ions (Mg2+)...
Anoxic depolarization of pyramidal neurons results from a large inward current that is activated, in part, by excessive glutamate release during exposure to anoxia/ischemia. Pannexin-1 (Panx1) channels can be activated both ischemia and NMDA receptors (NMDARs), but the mechanisms Panx1 activation are unknown. We used whole-cell recordings show pharmacological inhibition or conditional genetic deletion strongly attenuates anoxic CA1 acute brain slices rats mice. Anoxia exogenous Src family...
Abstract Mammalian gap junction proteins, connexins, have long been implicated in tumor suppression. Recently, a novel family of proteins named pannexins has identified as the mammalian counterpart invertebrate innexins. To date, pannexin 1 (Panx1) and 2 (Panx2) mRNAs are reported to be expressed brain. Most neoplastic cells, including rat C6 gliomas, exhibit reduced connexin expression, aberrant junctional intercellular communication (GJIC), an increased proliferation rate. When junctions...
Abstract Astrocytes support the energy demands of synaptic transmission and plasticity. Enduring changes in efficacy are highly sensitive to stress, yet whether astrocyte bioenergetic control synapses contributes stress-impaired plasticity is unclear. Here we show mice that stress constrains shuttling glucose lactate through networks, creating a barrier for neuronal access an astrocytic reservoir hippocampus neocortex, compromising long-term potentiation. Impairing delivery substrates by...
The ATP-releasing channel Panx1 is specifically involved in blood pressure regulation by adrenergic signaling.
Functional hyperemia occurs when enhanced neuronal activity signals to increase local cerebral blood flow (CBF) satisfy regional energy demand. Ca
1. We investigated whether adrenomedullary chromaffin cells (AMCs) derived from neonatal (postnatal day (P) 1‐P2) and juvenile (P13‐P20) rats, maintained in short‐term culture (1‐3 days), express O2‐chemoreceptive properties. 2. In whole‐cell recordings, the majority (approximately 70%; n = 47) of AMCs were sensitive to hypoxia. Under voltage clamp, acute hypoxia (PO2 approximately 40 mmHg) suppressed voltage‐dependent K+ current by 25.1 +/‐ 3.4% (mean S.E.M.; 22); under caused a membrane...
Despite increased risk of a recurrent stroke following minor stroke, information is minimal regarding the interaction between injurious mild cerebral ischemic episodes and possible treatments which might be effective. The aim current study was to investigate whether resveratrol, nutritive polyphenol with promising cardio- neuro- protective properties, could ameliorate associated brain damage. Experiments in adult rats demonstrated that followed by second ischemia exacerbated damage, and,...
Tolerance to the analgesic effects of opioids is a major problem in chronic pain management. Microglia are implicated opioid tolerance, but core mechanisms regulating their response remain obscure. By selectively ablating microglia spinal cord using saporin-conjugated antibody Mac1, we demonstrate causal role for development, not maintenance, morphine tolerance male rats. Increased P2X7 receptor (P2X7R) activity cardinal feature microglial activation, and this study found that potentiates...
Dietary and metabolic therapies are increasingly being considered for a variety of neurological disorders, based in part on growing evidence the neuroprotective properties ketogenic diet (KD) ketones. Earlier, we demonstrated that ketones afford hippocampal synaptic protection against exogenous oxidative stress, but mechanisms underlying these actions remain unclear. Recent studies have shown may modulate neuronal firing through interactions with ATP-sensitive potassium (KATP) channels....
Neuronal hyperactivity is an early primary dysfunction in Alzheimer's disease (AD) humans and animal models, but effective neuronal hyperactivity-directed anti-AD therapeutic agents are lacking. Here we define a previously unknown mode of ryanodine receptor 2 (RyR2) control AD progression. We show that single RyR2 point mutation, E4872Q, which reduces open time, prevents hyperexcitability, hyperactivity, memory impairment, cell death, dendritic spine loss severe early-onset mouse model...
The impact of pannexin-1 (Panx1) channels on synaptic transmission is poorly understood. Here, we show that selective block Panx1 in single postsynaptic hippocampal CA1 neurons from male rat or mouse brain slices causes intermittent, seconds long increases the frequency sEPSC following Schaffer collateral stimulation. increase occurred without an effect evoked neurotransmission. Consistent with a presynaptic origin augmented glutamate release, increased was prevented by bath-applied EGTA-AM...
Abstract Integration between neural and immune systems is critical for gastrointestinal functions, disruptions of this communication contribute to inflammatory bowel disease (IBD). Pannexin-1 (Panx1) a membrane channel expressed by enteric neurons cells that contributes intercellular regulation intestinal responses. Although Panx1 expression reduced in IBD its activation promotes neurodegeneration colitis, the specific contribution neuronal function remains unknown. Here, we tested...