A5037533745- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Retinal Development and Disorders
- bioluminescence and chemiluminescence research
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Photosynthetic Processes and Mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Aquaculture disease management and microbiota
- Plant Pathogens and Fungal Diseases
- Cell Image Analysis Techniques
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Vibrio bacteria research studies
- Machine Learning in Bioinformatics
- Nematode management and characterization studies
- Light effects on plants
University of Alberta
2016-2025
Simon Fraser University
2022-2024
Dalian Ocean University
2021-2024
Alberta Hospital Edmonton
2021
Abstract We developed a system for optogenetic release of single molecules in cells. confined soluble and transmembrane proteins to the Golgi apparatus via photocleavable protein released them by short pulses light. Our method allows light dose-dependent delivery functional cytosol plasma membrane amounts compatible with single-molecule imaging, greatly simplifying access microscopy any live were able reconstitute ion conductance delivering BK LRRC8/volume-regulated anion channels membrane....
Recombinant optogenetic and chemogenetic proteins are potent tools for manipulating neuronal activity controlling neural circuit function. However, there few analogous the structure of circuits. Here, we introduce three rationally designed genetically encoded that use E3 ligase-dependent mechanisms to trigger degradation synaptic scaffolding proteins, leading functional ablation synapses. First, developed a constitutive excitatory synapse ablator, PFE3, inhibitory ablator GFE3. PFE3 targets...
Photocleavable proteins can enable the light-dependent modulation of biomolecular activities with high spatiotemporal precision.
A severe disease occurred in farmed Eriocheir sinensis characterized by milky liquid accumulation the pectoral cavity, province of Liaoning, China, during October 2018-April 2019. Diseased crabs moved sluggishly, exhibited appetite loss and readily lost legs. Under microscopic analysis, it was observed that contained a large number yeastlike microorganisms (0.8-1.2 μm × 1.5-1.9 μm), which were also present muscle, hepatopancreas gills. dominant strain isolated from other tissues diseased...
Fluorogenic RNA aptamer tags with high affinity enable purification and imaging. The G-quadruplex (G4) based Mango (M) series of aptamers were selected to bind a thiazole orange (TO1-Biotin) ligand. Using chemical biology reselection approach, we have produced MII.2 aptamer-ligand complex remarkable set properties: Its unprecedented KD 45 pM, formaldehyde resistance (8% v/v), temperature stability ligand photo-recycling properties are all unusual find simultaneously within small tag. Crystal...
Abstract The photocleavable protein (PhoCl) is a green-to-red photoconvertible fluorescent that, when illuminated with violet light, undergoes main chain cleavage followed by spontaneous dissociation of the resulting fragments. first generation PhoCl (PhoCl1) exhibited relative slow rate dissociation, potentially limiting its utilities for optogenetic control cell physiology. In this work, we report X-ray crystal structures PhoCl1 green state, red and cleaved empty barrel. Using...
Takifugu rubripes is an important mariculture species, but development of its booming aquaculture industry has been accompanied by a serious disease problem. The bacterium Vibrio harveyi one the most pathogens T. rubripes. To gain more comprehensive understanding molecular mechanism responsible for intestinal mucosal immunity during V. infection, we established transcriptome posterior intestine after 0, 3, and 24 h infection with harveyi. Transcriptome analysis revealed 3042 3342...
Abstract Recombinant optogenetic and chemogenetic proteins are potent tools for manipulating neuronal activity controlling neural circuit function. However, there few analogous the structure of circuits. Here, we introduce three rationally designed genetically encoded that use E3 ligase-dependent mechanisms to trigger degradation synaptic scaffolding proteins, leading functional ablation synapses. First, developed a constitutive excitatory synapse ablator, PFE3, inhibitory ablator GFE3. PFE3...
Recombinant optogenetic and chemogenetic proteins that manipulate neuronal activity are potent tools for activating inhibiting circuit function. However, there few analogous manipulating the structure of neural circuits. Here, we introduce three rationally designed genetically encoded use E3 ligase-dependent mechanisms to trigger degradation synaptic scaffolding proteins, leading functional ablation synapses. First, developed a constitutive excitatory synapse ablator, PFE3, inhibitory...
Recombinant optogenetic and chemogenetic proteins that manipulate neuronal activity are potent tools for activating inhibiting circuit function. However, there few analogous manipulating the structure of neural circuits. Here, we introduce three rationally designed genetically encoded use E3 ligase-dependent mechanisms to trigger degradation synaptic scaffolding proteins, leading functional ablation synapses. First, developed a constitutive excitatory synapse ablator, PFE3, inhibitory...
We developed a system for optogenetic release of single molecules in live cells. confined soluble and transmembrane proteins to the Golgi apparatus via photocleavable protein released them by short pulses light. Our method allows controlled delivery functional cytosol plasma membrane amounts compatible with molecule imaging, greatly simplifying access microscopy any Furthermore, we could reconstitute cellular functions such as ion conductance delivering BK VRAC channels membrane. Finally,...