A5102980695- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Metal-Catalyzed Oxygenation Mechanisms
- Lymphoma Diagnosis and Treatment
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Pharmacogenetics and Drug Metabolism
- Chronic Lymphocytic Leukemia Research
- Catalytic C–H Functionalization Methods
- Synthesis and Catalytic Reactions
- Microbial Metabolites in Food Biotechnology
- Microbial Natural Products and Biosynthesis
- Protein Structure and Dynamics
- Seaweed-derived Bioactive Compounds
- Advanced Cellulose Research Studies
- Polysaccharides and Plant Cell Walls
- Traditional Chinese Medicine Analysis
- Identification and Quantification in Food
- Electrospun Nanofibers in Biomedical Applications
- Computational Drug Discovery Methods
- Renal Diseases and Glomerulopathies
- Cancer Immunotherapy and Biomarkers
- Land Use and Ecosystem Services
- RNA and protein synthesis mechanisms
Chongqing University of Posts and Telecommunications
2025
Fudan University Shanghai Cancer Center
2018-2025
Chinese Academy of Sciences
2015-2025
China University of Mining and Technology
2025
Aerospace Information Research Institute
2025
Qingdao Institute of Bioenergy and Bioprocess Technology
2017-2024
Qinghai New Energy (China)
2022-2024
University of Chinese Academy of Sciences
2020-2024
Affiliated Hospital of Shaanxi University of Chinese Medicine
2019-2024
Dalian Ocean University
2024
Understanding the molecular basis for controlled H2O2 activation is of fundamental importance peroxide-driven catalysis by metalloenzymes. In addition to O2 in presence stoichiometric reductants, an increasing number metalloenzymes are found activate cosubstrate oxidative transformations absence reductants. Herein, we characterized X-ray structure P450BM3 F87A mutant complex with dual-functional small molecule (DFSM) N-(ω-imidazolyl)-hexanoyl-l-phenylalanine (Im-C6-Phe), which enables...
Applications of the peroxidase activity cytochrome P450 enzymes in synthetic chemistry remain largely unexplored. We present herein a protein engineering strategy to increase P450BM3 for direct nitration aromatic compounds and terminal aryl-substituted olefins presence dual-functional small molecule (DFSM). Site-directed mutations key active-site residues allowed efficient regulation steric effects limit substrate access and, thus, significant decrease monooxygenation activity. Nitration...
It is a great challenge to optionally access diverse hydroxylation products from given substrate bearing multiple reaction sites of sp3 and sp2 C-H bonds. Herein, we report the highly selective divergent alkylbenzenes by an engineered P450 peroxygenase driven dual-functional small molecule (DFSM). Using combinations various P450BM3 variants with DFSMs enabled more than half all possible hydroxylated each excellent regioselectivity (up >99 %), enantioselectivity % ee), high total turnover...
Microbial epoxide hydrolases, cis-epoxysuccinate hydrolases (CESHs), have been utilized for commercial production of enantiomerically pure L(+)- and D(-)-tartaric acids decades. However, the stereo-catalytic mechanism CESH producing L(+)-tartaric acid (CESH[L]) remains unclear. Herein, crystal structures two CESH[L]s in ligand-free, product-complexed, catalytic intermediate forms were determined. These revealed unique specific binding mode mirror-symmetric substrate, an active triad...
Cellulosomes are intricate cellulose-degrading multi-enzymatic complexes produced by anaerobic bacteria, which valuable for bioenergy development and biotechnology. Cellulosome assembly relies on the selective interaction between cohesin modules in structural scaffolding proteins (scaffoldins) dockerin enzymes. Although number of tandem cohesins scaffoldins is believed to determine complexity cellulosomes, dockerins also exist, albeit very rare, some cellulosomal components whose functional...
Effective production, living, and ecological space allocation is essential for improving optimizing urban development. In this study, we proposed a production–living–ecological (PLES) identification method based on Point of Interest (POI) data China Land Cover Dataset (CLCD) to identify PLESs in Xuzhou City the years 2012, 2018, 2022, with an average recognition accuracy 89.81%. Moreover, land-use transfer matrix, center gravity migration, Geo-detector were used reveal spatiotemporal pattern...
Epstein-Barr virus (EBV)-positive diffuse large B-cell lymphoma (DLBCL) is a highly aggressive malignancy with inferior outcomes after treatment, which might be largely attributed to the immune escape induced by EBV via modulation of checkpoint programmed cell death 1 (PD-1)/PD-ligand (PD-L1). This study aimed explore role that EBV-encoded latent membrane protein 2A (LMP2A) played in mechanisms EBV+ DLBCL cells. Ten cases each and without immunohistochemical expression LMP2A were submitted...
Workplace violence in healthcare has become a global concern, and nursing interns are not immune to it clinical settings. It is critical understand the impact of workplace on psychological work status interns. Between April May 2024, total 2213 from 12 hospitals China participated study. Data were collected with questionnaires basic information, Violence Scale, Caregiver Occupational Deviant Behavior DSM-5 Level 1 Cross-Cutting Symptom Measure. The analysis was performed using SPSS 24.0...
Autoproteolysis has been discovered to play key roles in various biological processes, but functional autoproteolysis rarely reported for transmembrane signaling prokaryotes. In this study, an autoproteolytic effect was the conserved periplasmic domain of anti-σ factor RsgIs from Clostridium thermocellum, which found transmit extracellular polysaccharide-sensing signals into cells regulation cellulosome system, a polysaccharide-degrading multienzyme complex. Crystal and NMR structures...
In this paper, we describe the newly designed liposomes modified with amphiphilic far-red squaraine dye and folic acid for its application in folate receptor-targeted bioimaging. Enhanced intracellular uptake of engineered has been demonstrated on SKOV-3 ovarian cancer cells.
A pH-dependent dual-binding-site switch in a pair of protein modules is discovered, and the molecular mechanism elucidated.
Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because its cellulosomal system. As major exoglucanase cellulosome in C. thermocellum, Cel48S plays key roles and influences activity features to a great extent. Thus, it importance reveal enzymatic Cel48S. However, has not been well performed due difficulties purifying either recombinant or native proteins.We observed that soluble fraction catalytic domain (Cel48S_CD) obtained by heterologous...
Significance Selective oxidation of aliphatic C–H bonds in alkylphenols is important for both structural derivatization and biological degradation these fundamental chemicals. However, significant problems are persistently associated with the chemical methods this oxofunctionalization. In study, we developed a unique chemomimetic biocatalytic system that capable selectively oxidizing p - m -alkylated phenols controllable manner, overcoming challenges faced by similar oxidation. The...
The σ70 family alternative σI factors and their cognate anti-σI are widespread in Clostridia Bacilli play a role heat stress response, virulence, polysaccharide sensing. Multiple σI/anti-σI exist some lignocellulolytic clostridial species, specifically for regulation of components multienzyme complex, termed the cellulosome. unique, because C-terminal domain (SigIC) N-terminal inhibitory (RsgIN) lack homology to known proteins. Here, we report structure interaction studies pair factors,...
Background Protectin D1 (PD1), derived from docosahexaenoic acid, has been shown to control and resolve inflammation in some experimental models of inflammatory disorders. We investigated the protective roles protectin pulmonary lung injury induced by lipopolysaccharide (LPS). Methods Mice were randomly assigned six groups ( n =6 per group): sham-vehicle group, sham-PD1 sham-zVAD-fmk LPS-vehicle LPS-PD1 LPS-PD1-zVAD-fmk group. injected intratracheally with 3 mg/kg LPS or saline, followed 24...
Glycoside hydrolase (GH) family 5 is one of the largest GH families with various activities including lichenase, but structural basis GH5 lichenase activity still unknown. A novel thermostable F32EG5 belonging to was identified from an extremely thermophilic bacterium Caldicellulosiruptor sp. F32. a bi-functional cellulose and lichenan-degrading enzyme, exhibited high on β-1,3-1,4-glucan side cellulose. Thin-layer chromatography NMR analyses indicated that cleaved β-1,4 linkage or β-1,3...
All O-methylated derivatives of emodin, including physcion, questin, and 1-O-methylemodin, show potential antifungal activities. Notably, emodin questin are two pivotal intermediates geodin biosynthesis in Aspergillus terreus. Although most the biosynthetic steps have been investigated, key O-methyltransferase (OMT) responsible for O-methylation to generate has remained unidentified. Herein, through phylogenetic tree analysis vitro biochemical assays, long-sought class II...
Abstract Bacterial cis -epoxysuccinic acid hydrolases (CESHs) are intracellular enzymes used in the industrial production of enantiomeric tartaric acids. The mainly as whole-cell catalysts because low stability purified CESHs. However, cell permeability is major drawback catalyst. To overcome this problem, we developed using various surface display systems for CESH[L] which produces L(+)-tartaric acid. Considering that efficiency depends on both carrier and passenger, screened five different...