A5100631593- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Crystallography and molecular interactions
- Protein Structure and Dynamics
- Enzyme Production and Characterization
- biodegradable polymer synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Hemoglobin structure and function
- Bacterial Genetics and Biotechnology
- Chalcogenide Semiconductor Thin Films
- RNA and protein synthesis mechanisms
- Advanced Nanomaterials in Catalysis
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Circular RNAs in diseases
- Phytase and its Applications
- Quantum Dots Synthesis And Properties
- Nanocluster Synthesis and Applications
- Hepatitis B Virus Studies
- Protein Hydrolysis and Bioactive Peptides
- Carbon dioxide utilization in catalysis
- Metabolomics and Mass Spectrometry Studies
- Neuroscience and Neuropharmacology Research
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
Chinese Academy of Sciences
2013-2025
Center for Excellence in Brain Science and Intelligence Technology
2024-2025
University of Chinese Academy of Sciences
2009-2025
Stony Brook University
2025
Qingdao Institute of Bioenergy and Bioprocess Technology
2019-2024
Huangshi Central Hospital
2024
Hubei Polytechnic University
2024
Shandong First Medical University
2024
Changchun Institute of Optics, Fine Mechanics and Physics
2023
PLA Navy General Hospital
2023
The histopathological neurons in the brain tissue of drug-resistant epilepsy exhibit aberrant cytoarchitecture and imbalanced synaptic circuit function. However, gene expression changes these remain unknown, making it difficult to determine diagnosis or dissect mechanism epilepsy. By integrating whole-cell patch clamp recording single-cell RNA-seq approaches, we identified a transcriptionally distinct subset cortical pyramidal neurons. These highly expressed genes CDKN1A (P21), CCL2, NFKBIA,...
Cu2ZnSn(S1−xSex)4 nanocrystals are an emerging family of functional materials with huge potential industrial applications, however, it is extremely challenging task to synthesize both tunable energy band and phase purity. Here we show that a green economic route could be designed for the synthesis bandgap in range 1.5–1.12 eV. Consequently, conduction edge shifted from −3.9 eV −4.61 (relative vacuum energy) realized. The purity substantiated in-depth combined optical structural...
Harvesting a remarkable antibacterial performance is major point to choose enzymatic catalytic and photocatalytic coupling catalysts. Here, Co–Mo–S mixed metal sulfides (MMSs) were composed by tailoring the composition energy of organic framework (MOF)-based precursors. When n(Co)/n(Mo) = 1:1, Co/Mo-MOF in lowest state vulcanized CoS2/MoS2 nanosheets (NSs) exhibit best property. With inherent activity, NSs can degrade more than 90% methylene blue, methyl orange, rhodamine B, tetracycline...
Cellulosomes are intricate cellulose-degrading multi-enzymatic complexes produced by anaerobic bacteria, which valuable for bioenergy development and biotechnology. Cellulosome assembly relies on the selective interaction between cohesin modules in structural scaffolding proteins (scaffoldins) dockerin enzymes. Although number of tandem cohesins scaffoldins is believed to determine complexity cellulosomes, dockerins also exist, albeit very rare, some cellulosomal components whose functional...
Abstract Background The examination of high-risk cells and regions in tissue samples from spatially resolved transcriptomics platforms, offers meaningful insights into specific disease processes. For existing methods, while cell types or clusters can be identified associated with attributes, individual are unable to the same manner which may result failing identify subsets attributes especially if disease-associated cluster together non-disease-associated cells. Method DEGAS (Diagnostic...
The intensified charge-coupled device (ICCD), known for its exceptional low-light detection performance and time-gating capability, has been widely applied in remote Raman spectroscopy systems. However, existing ICCDs face significant challenges meeting the comprehensive requirements of high gating speed, sensitivity, resolution, miniaturization, adaptability to extreme environments upcoming lunar missions. To address these challenges, this study developed a microstrip photocathode (MP-ICCD)...
Autoproteolysis has been discovered to play key roles in various biological processes, but functional autoproteolysis rarely reported for transmembrane signaling prokaryotes. In this study, an autoproteolytic effect was the conserved periplasmic domain of anti-σ factor RsgIs from Clostridium thermocellum, which found transmit extracellular polysaccharide-sensing signals into cells regulation cellulosome system, a polysaccharide-degrading multienzyme complex. Crystal and NMR structures...
As a dominating air pollutant, atmospheric fine particulate matter within 2.5 μm in diameter (PM2.5) has attracted increasing attention from the researchers all over world, which will lead to various adverse effects on central nervous system (CNS), yet potential mechanism is unclear. In this study, microglia (BV2 cell line) were exposed different concentrations of PM2.5 (5, 10 and 20 μg/cm2) for 24 h. It was found that could result such as decreased viability, structural damage even death....
Ternary semiconductor nanocrystals from the I–V–VI family have potential applications in many fields. In this work, we report on synthesis of well-dispersed Cu3SbS3 with an average diameter 23.0 ± 4.9 nm. The as-synthesized are high phase purity and stable against separation when heating up to 400 °C inert atmosphere or left at room for 80 days. optical band gap is calculated as ~1.87 eV. nanocrystal film shows strong near infrared absorption sensitive towards detecting trace amounts...
A pH-dependent dual-binding-site switch in a pair of protein modules is discovered, and the molecular mechanism elucidated.
The gradual degradation of polyester in daily use causes unpredictable failure and restricts its reliable applications. realization excellent stability controlled a specific environment is highly appreciated but still remains challengeable. Here, we demonstrate new solution via introducing acetal units into poly(butylene succinate): "locking" the biodegradation copolyesters by rigid spiro diacetal, which could be "unlocked" strong acidic circumstances. In detail, series...
The σ70 family alternative σI factors and their cognate anti-σI are widespread in Clostridia Bacilli play a role heat stress response, virulence, polysaccharide sensing. Multiple σI/anti-σI exist some lignocellulolytic clostridial species, specifically for regulation of components multienzyme complex, termed the cellulosome. unique, because C-terminal domain (SigIC) N-terminal inhibitory (RsgIN) lack homology to known proteins. Here, we report structure interaction studies pair factors,...
Circular RNAs (circRNAs) exhibited important roles in Alzheimer's disease (AD). Here, we focused on the dysregulation of hsa_circ_0049472 (circ_0049472) and potential functions SK-N-SH cells with amyloid-beta peptide (Aβ) treatment AD. RNA expression was detected by real-time quantitative PCR. Cell viability proliferation were measured MTS Edu assays. Flow cytometry used for apoptosis detection, cell inflammation assessed using enzyme-linked immunosorbent assay. Target interaction validated...
Abstract Background Lysine succinylation is a naturally occurring post-translational modification (PTM) that ubiquitous in organisms. plays important roles regulating protein structure and function as well cellular metabolism. Global lysine at the proteomic level has been identified variety of species; however, limited information on plant species, especially paper mulberry, available. Paper mulberry not only an traditional Chinese medicine, but it also tree species with significant economic...
While two-dimensional infrared (2D-IR) spectroscopy is uniquely suitable for monitoring femtosecond (fs) to picosecond (ps) water dynamics around static protein structures, its utility probing enzyme active-site limited due the lack of site-specific 2D-IR probes. We demonstrate genetic incorporation a novel probe, m-azido-L-tyrosine (N3Y) in DddK, an iron-dependent that catalyzes conversion dimethylsulfoniopropionate dimethylsulphide. Our results show both oxidation iron FeIII , and addition...
A new constitutive equation of thermoelasticity for crystals is presented based on the interatomic potential and solid mechanics at finite temperature. Using equation, calculations crystal copper graphene are carried out under different loading paths temperatures. The calculated results in good agreement with those previous quantum mechanics, which clearly indicates that our correct. lot comparisons also show present theory more concise efficient than thermal stress practical application.
Tyrosine plays important roles in many enzymes. To facilitate enzyme design, mechanistic studies and minimize structural perturbation the active site, here we report genetic incorporation of a novel unnatural amino acid selenotyrosine (SeHF), which has single-atom replacement comparison to tyrosine. The arPTE-(Agrobacterium radiobacter Phosphotriesterase) Tyr309SeHF mutant exhibits significant 12-fold increase k