A5111944814- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Algal biology and biofuel production
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Advanced Cellulose Research Studies
- Enzyme Structure and Function
- Lipid metabolism and biosynthesis
- Polysaccharides and Plant Cell Walls
- Catalysis for Biomass Conversion
- Microbial Metabolites in Food Biotechnology
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Clostridium difficile and Clostridium perfringens research
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Lignin and Wood Chemistry
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Plant biochemistry and biosynthesis
- Toxin Mechanisms and Immunotoxins
Chinese Academy of Sciences
2016-2025
Qingdao Institute of Bioenergy and Bioprocess Technology
2016-2025
University of Chinese Academy of Sciences
2020-2025
Qinghai New Energy (China)
2021-2025
Shandong University
2024
State Key Laboratory of Microbial Technology
2024
Dalian National Laboratory for Clean Energy
2018-2023
Ningbo Institute of Industrial Technology
2021-2023
Collaborative Innovation Center of Advanced Microstructures
2023
Nanjing University
2023
Polyethylene terephthalate (PET) is a mass-produced synthetic polyester contributing remarkably to the accumulation of solid plastics waste and pollution in natural environments. Recently, bioremediation using engineered enzymes has emerged as an eco-friendly alternative approach for future plastic circular economy. Here we genetically thermophilic anaerobic bacterium, Clostridium thermocellum, enable secretory expression cutinase (LCC), which was originally isolated from plant compost...
The hydrolysis and biotransformation of lignocellulose, i.e., biorefinery, can provide human beings with biofuels, bio-based chemicals, materials, is an important technology to solve the fossil energy crisis promote global sustainable development. Biorefinery involves steps such as pretreatment, saccharification, fermentation, researchers have developed a variety biorefinery strategies optimize process reduce costs in recent years. Lignocellulosic hydrolysates are platforms that connect...
The bioconversion of lignocellulose has attracted global attention, due to the significant potential agricultural and forestry wastes as renewable zero-carbon resources urgent need for substituting fossil carbon. cellulosome system is a multi-enzyme complex produced by anaerobic bacteria, which comprises cellulases, hemicellulases, associated enzymatic non-enzymatic components that promote biomass conversion. To enhance their efficiency in degrading recalcitrant lignocellulosic matrices,...
Recently, a solar-driven evaporator has been applied in seawater desalination, but the low stability, high cost, and complex fabrication limit its further application. Herein, we report novel, low-cost, scalable, easily fabricated pulp-natural rubber (PNR) foam with unique porous structure, which was directly used as after facile surface carbonization. This carbonized PNR (CPNR) without interface adhesion or modification composed of top photothermal layer light absorption ability bottom...
A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis 18S rDNA SD116 showed that has a close phylogenetic relationship Aurantiochytrium species. The highest rates for growth and DHA accumulation were obtained in 6.0% glucose, 2.0% yeast extract, 50% artificial seawater (ASW) at pH 7 28°C. maximum total lipid content reached 56.3%...
Abstract Aurantiochytrium is a promising docosahexaenoic acid (DHA) production candidate due to its fast growth rate and high proportions of lipid DHA content. In this study, high-throughput RNA sequencing technology was employed explore the acclimatization producer under cold stress at transcriptional level. The overall de novo assembly cDNA sequence data generated 29,783 unigenes, with an average length 1,200 bp. total, 13,245 unigenes were annotated in least one database. A comparative...
Corncob residue (CCR) is an economic feedstock with enormous potential for the production of bioethanol. In this work, potassium hydroxide (KOH) pretreatment CCR was investigated under relatively mild conditions, and effectiveness KOH evaluated by enzymatic saccharification at high solid loading. Results showed that after (16 wt% dosage 70 °C 90 min) hydrolysis 20% solids loading 20 FPU g-1-substrate cellulase loading, glucose yield could reach up to about 91%, which over 90% higher compared...
Background Targetrons are gene targeting vectors derived from mobile group II introns. They consist of an autocatalytic intron RNA (a "ribozyme") and intron-encoded reverse transcriptase, which use their combined activities to achieve highly efficient site-specific DNA integration with readily programmable target specificity. Methodology/Principal Findings Here, we used a the thermophilic cyanobacterium Thermosynechococcus elongatus construct thermotargetron for in thermophiles. After...
Clostridium thermocellum is a thermophilic anaerobic bacterium that degrades cellulose by using highly effective cellulosome, macromolecular complex consisting of multiple degrading enzymes organized and attached to the cell surface non-catalytic scaffoldins. However, due largely lack efficient methods for genetic manipulation C. thermocellum, it still unclear how different scaffoldins their functional modules contribute hydrolysis.We constructed mutants with primary scaffoldin CipA...
Abstract The mechanism, physiological relevance and evolutionary implication of selective RNA processing stabilization (SRPS) remain elusive. Here we report the genome-wide maps transcriptional start sites (TSs) post-transcriptional processed (PSs) for Clostridium cellulolyticum . PS-associated genes are preferably associated with subunits heteromultimeric protein complexes, intergenic PSs (iPSs) enriched in operons exhibiting highly skewed transcript-abundance landscape. Stem-loop...
Eicosapentaenoic acid (EPA) is an essential nutritional supplement for human health. The most prominent dietary source of EPA fish oil, which unsustainable because the decline in fishery resources and serious environmental pollution. Alternatively, a heterologous polyketide synthase pathway biosynthesis was assembled Thraustochytrid Aurantiochytrium. A 2A peptide-based facile assembly platform that can achieve multigene expression as polycistron first established. then applied to express...
Dialdehyde nanocellulose (DANC), obtained via periodate oxidation, has been widely used in foods, cosmetics, and biomedical fields due to its unique characteristics. However, the high-crystallinity tight structure of cellulose retards efficiency oxidation for producing DANC. Herein, a novel sustainable clean approach with less energy consumption low cost was developed through LiBr·3H2O (LBTH) non-dissolving pretreatment, which can shorten time followed reduce oxidant consumption. It shown...
Cellulosomes are intricate cellulose-degrading multi-enzymatic complexes produced by anaerobic bacteria, which valuable for bioenergy development and biotechnology. Cellulosome assembly relies on the selective interaction between cohesin modules in structural scaffolding proteins (scaffoldins) dockerin enzymes. Although number of tandem cohesins scaffoldins is believed to determine complexity cellulosomes, dockerins also exist, albeit very rare, some cellulosomal components whose functional...
Aurantiochytrium is an important docosahexaenoic acid (DHA) producer containing two kinds of fatty synthesis pathways, that is, the synthase pathway (FAS) for saturated and polyketide (PKS) polyunsaturated synthesis. To understand regulation mechanism between impacts six short-chain acids on sp. SD116 were studied. All showed little effect cell growth, but some them significantly affected lipid accumulation composition. Pentanoic isovaleric greatly inhibited acids, whereas was not affected....
Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades efficiently using cellulosome, a multiprotein supermolecular complex, and thus can be potentially used as low-cost catalyst saccharification. The use C. restrained due largely to inhibition hydrolysate cellobiose its cellulosome. Although supplementation beta-glucosidase may solve problem, production enzymes greatly complicates process also...