A5044969657- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- interferon and immune responses
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Immune cells in cancer
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Skin and Cellular Biology Research
- Caveolin-1 and cellular processes
- Curcumin's Biomedical Applications
- Retinal Development and Disorders
- DNA Repair Mechanisms
- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- bioluminescence and chemiluminescence research
- Receptor Mechanisms and Signaling
- Cardiomyopathy and Myosin Studies
- Cardiovascular Issues in Pregnancy
- Bioactive Compounds and Antitumor Agents
- Nuclear Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Retinoids in leukemia and cellular processes
- AI in cancer detection
Max Planck Institute for Infection Biology
2020-2024
Charité - Universitätsmedizin Berlin
2020-2023
Zhejiang University of Technology
2022
University of Hong Kong
2015-2020
Henan University of Technology
2015
Abstract The turnover of integrin receptors is critical for cell migration and adhesion dynamics. Here we find that force development at integrins regulates adaptor protein recruitment endocytosis. Using mobile RGD (Arg-Gly-Asp) ligands on supported lipid membranes (RGD membranes) rigid glass (RGD-glass), matrix force-dependent signals block Dab2, an clathrin-mediated endocytosis, not recruited to activated integrin-beta3 clusters RGD-glass; however, it integrin-mediated adhesions membranes....
A recurring feature of innate immune receptor signaling is the self-assembly proteins into oligomeric complexes. The Myddosome an complex that required to transmit inflammatory signals from TLR/IL1Rs and consists MyD88 IRAK family kinases. However, molecular basis for how self-assemble regulate intracellular remains poorly understood. Here, we developed a novel assay analyze spatiotemporal dynamics IL1R in live cells. We found oligomerization inducible initially reversible. Moreover,...
Abstract We developed a system for optogenetic release of single molecules in cells. confined soluble and transmembrane proteins to the Golgi apparatus via photocleavable protein released them by short pulses light. Our method allows light dose-dependent delivery functional cytosol plasma membrane amounts compatible with single-molecule imaging, greatly simplifying access microscopy any live were able reconstitute ion conductance delivering BK LRRC8/volume-regulated anion channels membrane....
ABSTRACT Podosomes play crucial roles in macrophage adhesion and migration. Wiskott–Aldrich syndrome protein (WASP; also known as WAS)-mediated actin polymerization is one of the key events initiating podosome formation. Nevertheless, membrane signals to trigger WASP activation at podosomes remain unclear. Here, we show that phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] lipids are enriched stably recruit rather than WASP-5KE mutant. Phosphatidylinositol 4,5-bisphosphate 3-kinase...
During podosome formation, distinct phosphatidylinositol 3,4,5-trisphosphate lipid (PI(3,4,5)P3) production and F-actin polymerization take place at integrin-mediated adhesions. Membrane-associated actin regulation factors, such as myosin-1, serve key molecules to link signals assembly. Here, we report that long-tailed myosin-1e (Myo1e) is enriched the ventral layer of core in a PI(3,4,5)P3-dependent manner. The combination TH1 TH2 (TH12) Myo1e tail domains contains essential motif for...
Abstract Integrin receptors orchestrate cell adhesion and cytoskeletal reorganization. The endocytic mechanism of integrin-β3 receptor at the podosome remains unclear. Using viscous RGD-membrane as model system, here we show that formation podosome-like promotes Dab2/clathrin-mediated endocytosis integrin-β3. Integrin-β3 RGD ligand are endocytosed from sorted into endosomal compartment. Inhibitions knockdowns Dab2 clathrin reduce endocytosis. F-actin assembly core exhibits protrusive contact...
IL-1 receptor (IL-1R) signaling can activate thresholded invariant outputs and proportional that scale with the amount of stimulation. Both responses require Myddosome, a multiprotein complex. The Myddosome is required for polyubiquitin chain formation NF-kB signaling. However, how these signals are spatially temporally regulated to drive switch-like outcomes not understood. During IL-1R signaling, Myddosomes dynamically reorganize into multi-Myddosome clusters at cell membrane. Blockade...
Abstract A key feature of innate immune signaling is the compartmentalization effectors into cellular structures referred to as signalosomes. Critical formation these compartments are protein polymers composed Death Domains (DD). However, biophysical properties polymeric scaffolds require for signal transduction not clearly defined. Here, we engineered a single-component signalosome, Chimeric Higher-order Assemblies Receptor Mediated Signaling (CHARMS). We found that CHARMS functionality...
The RING finger protein TRAIP protects genome integrity and its mutation causes Seckel syndrome. encodes a nucleolar that migrates to UV-induced DNA lesions via direct interaction with the replication clamp PCNA. Thus far, mechanistically how UV mobilizes from nucleoli remains unknown. We found PCNA binding is dispensable for nucleolus-nucleoplasm shuttling of following cell exposure irradiation, redistribution did not rely on master damage kinases ATM ATR. Interestingly, I-PpoI-induced...
Abstract Signaling pathways can produce digital invariant outputs and analog that scale with the amount of stimulation. In IL-1 receptor (IL-1R) signaling both types require Myddosome, a multi-protein complex. The Myddosome is required for polyubiquitin chain formation NF-kB signaling. However, ways in which these signals are spatially temporally regulated to drive switch-like proportional outcomes not understood. We find during IL-1R signaling, Myddosomes dynamically re-organize into large,...
Abstract A recurring feature of innate immune receptor signaling is the self-assembly proteins into oligomeric complexes. The Myddosome an complex that required to transmit inflammatory signals from TLR/IL1Rs and consists MyD88 IRAK family kinases. However, molecular basis for how self-assemble regulate intracellular remains poorly understood. Here, we developed a novel assay analyze spatiotemporal dynamics IL1R in live cells. We found oligomerization inducible initially reversible....
Abstract A recurring feature of innate immune receptor signaling is the self-assembly proteins into oligomeric complexes. The Myddosome an complex that required to transmit inflammatory signals from TLR/IL1Rs and consists MyD88 IRAK family kinases. However, molecular basis for how self-assemble regulate intracellular remains poorly understood. Here, we developed a novel assay analyze spatiotemporal dynamics IL1R in live cells. We found oligomerization inducible initially reversible....
Histopathological imaging analysis of colorectal polyps is the gold standard for tumor diagnosis. Nevertheless, diagnostic process organizing images time-consuming and laborious. So a fast effective computer automatic method needed. In recent years, Convolutional Neural Networks (CNN) have been frequently used cancer classification tasks. potential global channel relationships image may be overlooked influence representation capability features.Also, results are often not convincing...
We developed a system for optogenetic release of single molecules in live cells. confined soluble and transmembrane proteins to the Golgi apparatus via photocleavable protein released them by short pulses light. Our method allows controlled delivery functional cytosol plasma membrane amounts compatible with molecule imaging, greatly simplifying access microscopy any Furthermore, we could reconstitute cellular functions such as ion conductance delivering BK VRAC channels membrane. Finally,...