A5101700553- Eating Disorders and Behaviors
- Ion channel regulation and function
- Psychosomatic Disorders and Their Treatments
- Obsessive-Compulsive Spectrum Disorders
- Cardiac electrophysiology and arrhythmias
- Child Nutrition and Feeding Issues
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- COVID-19 and Mental Health
- Autism Spectrum Disorder Research
- Migraine and Headache Studies
- Perfectionism, Procrastination, Anxiety Studies
- Impact of Technology on Adolescents
- Nicotinic Acetylcholine Receptors Study
- Transcranial Magnetic Stimulation Studies
- Body Image and Dysmorphia Studies
- Advanced Fluorescence Microscopy Techniques
- Ion Channels and Receptors
- Retinal Development and Disorders
- Bipolar Disorder and Treatment
- Workaholism, burnout, and well-being
- Clinical practice guidelines implementation
- Neuroscience and Neuropharmacology Research
- Female Genital Mutilation/Cutting Issues
- Psychoanalysis and Psychopathology Research
- Electrochemical Analysis and Applications
Humboldt State University
2024
Azienda Socio Sanitaria Territoriale Santi Paolo e Carlo
2017-2024
Ospedale San Paolo
1999-2024
Humboldt-Universität zu Berlin
2023-2024
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2022-2024
AOL (United States)
2024
Ospedale San Carlo
2023
Scuola Internazionale Superiore di Studi Avanzati
2018-2022
Instituto di Biofisica
2015-2022
National Research Council
2018-2022
Anorexia nervosa (AN) is a psychiatric disease with devastating physical consequences, pathophysiological mechanism still to be elucidated. Metagenomic studies on anorexia have revealed profound gut microbiome perturbations as possible environmental factor involved in the disease. In this study we performed comprehensive analysis integrating data microbiota clinical, anthropometric and psychological traits gain new insight pathophysiology of AN. Fifteen AN women were compared fifteen age-,...
A hypothesis that eating disorders are a phenomenological variant of obsessive-compulsive disorder (OCD) has been proposed. This study was conducted to determine whether anorexia nervosa and bulimia, the two main disorders, familial risk for spectrum (OCD tic disorders) is higher in families patients with disorders.The morbidity first-degree relatives 136 female probands (84 nervosa, 52 bulimia) compared 72 comparison subjects.The significantly among 436 than 358 subjects (9.69% versus 0%)....
Cortical spreading depression (CSD), a wave of depolarization followed by cortical activity, is pathophysiological process implicated in migraine with aura and various other brain pathologies, such as ischemic stroke traumatic injury. To gain insight into the pathophysiology CSD, we generated mouse model for severe monogenic subtype aura, familial hemiplegic type 3 (FHM3). FHM3 caused mutations SCN1A, encoding voltage-gated Na+ channel NaV1.1 predominantly expressed inhibitory interneurons....
Tumor microenvironments are often characterized by an increase in oxidative stress levels. We studied the response to stimulation human primary (IGR39) or metastatic (IGR37) cell lines obtained from same patient, performing patch-clamp recordings, intracellular calcium ([Ca2+]i) imaging, and RT-qPCR gene expression analysis. In IGR39 cells, chloramine-T (Chl-T) activated large K+ currents (KROS) that were partially sensitive tetraethylammonium (TEA). A fraction of KROS was inhibited...
To explore the prevalence of symptoms anxiety and depression, along with PTSD- ED-related symptoms, across a sample patients Eating Disorders (EDs) compared to group healthy controls (HC) during lockdown period in Italy; assess whether patients' reported aforementioned psychiatric improved, remained stable or worsened easing measures.t0 assessment (during lockdown): 59 ED 43 HC completed an online survey, including Depression, Anxiety Stress Scale-21 items (DASS-21), Impact Event...
Abstract We developed a system for optogenetic release of single molecules in cells. confined soluble and transmembrane proteins to the Golgi apparatus via photocleavable protein released them by short pulses light. Our method allows light dose-dependent delivery functional cytosol plasma membrane amounts compatible with single-molecule imaging, greatly simplifying access microscopy any live were able reconstitute ion conductance delivering BK LRRC8/volume-regulated anion channels membrane....
Abstract Ion channels are critical in enabling ion movement into and within cells important targets for pharmacological interventions different human diseases. In addition to their transport abilities, interact with signalling scaffolding proteins, which affects function, cellular positioning, links intracellular pathways. The study of “channelosomes” has the potential uncover involvement diseases, although this field research is still emerging. LRRC8A gene that encodes a crucial protein...
Background and purpose The volume regulated anion channel (VRAC) is known to be involved in different aspects of cancer cell behaviour response therapies. For this reason, we investigated the effect DCPIB, a presumably specific blocker VRAC, two types cancer: pancreatic duct adenocarcinoma (PDAC) melanoma. Experimental approach We used patch‐clamp electrophysiology, supported by Ca 2+ imaging, gene expression analysis, docking simulation mutagenesis. employed PDAC lines (Panc‐1 MiaPaCa‐2),...
Abstract Objective This quasi‐experimental study aimed to compare the outcome of patients with Anorexia Nervosa (AN) reporting moderate/severe childhood maltreatment (CM) treated exclusively Enhanced Cognitive Behaviour Therapy (CBT‐E) or CBT‐E plus Eye Movement Desensitisation and Reprocessing (EMDR). Method A total 75 AN CM were initially assessed regarding body mass index (BMI), general eating disorder (ED)‐specific psychopathology, dissociative symptoms, re‐evaluated after 40 sessions...
Probands affected with eating disorders (ED) present a higher number of relatives obsessive-compulsive disorders/tic than comparison population. Therefore, we hypothesized that ED and disorder (OCD) might share the same biological liability, single major gene account for liability. We tested this hypothesis by applying complex segregation analysis to 141 families probands (89 anorexia nervosa, restricting binge-eating types, 52 bulimia nervosa). Given relationship between OCD genetic...
Familial hemiplegic migraine 3 is an autosomal dominant headache disorder associated with aura and transient hemiparesis, caused by mutations of the neuronal voltage-gated sodium channel Nav1.1. While a gain-of function phenotype generally assumed to underlie familial migraine, this has not been fully explored. Indeed, major obstacle in studying vitro channels difficulty propagating mutagenizing expression plasmids containing their cDNAs. The aim work was study functional effect two...
We tested if there were any differences about nocturnal and diurnal anxiety between patients either affected by Binge Eating Disorder (BED) or Night eating Syndrome (NES). Fifty four BED, 13 NES 16 both BED using the Self Rating Anxiety Scale (SAS) Sleep Disturbance Questionnaire (SDQ). Their behavior was ascertained through (NEQ). Patients scored significantly higher on SAS than other patients. Among we found a correlation SDQ subscale two subscales of NEQ. scores ingestion Nocturnal is...
Familial hemiplegic migraine (FHM) is a group of genetic migraine, associated with hemiparesis and aura. Three causative different genes have been identified, all which are involved in membrane ion transport. Among these, SCN1A encodes the voltage-gated Na+ channel Nav1.1, FHM caused by mutations named FHM3. For 7 12 known FHM3-causing SCNA1 functional consequences investigated, even if gain function effect seems to be predominant phenotype, for several conflicting results obtained available...
The volume-regulated anion channel (VRAC) is formed by LRRC8 subunits. Besides their role in the maintenance of cell homeostasis, VRACs are critically involved oxidative stress mechanisms: reactive oxygen species directly modulate a subunit-dependent manner. It was reported that LRRC8A-LRRC8E heteromeric channels activated oxidation, whereas LRRC8A-LRRC8C heteromers inhibited. Here we adopted chimeric- as well concatemeric-based strategies to identify residues responsible for divergent...