A5057685639- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Advanced Materials and Mechanics
- Evolutionary Game Theory and Cooperation
- Evolution and Genetic Dynamics
- Cancer Research and Treatments
- Micro and Nano Robotics
- Immunotherapy and Immune Responses
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Ion Channels and Receptors
- Nicotinic Acetylcholine Receptors Study
- Fish Biology and Ecology Studies
- Pancreatic and Hepatic Oncology Research
- Genomics and Phylogenetic Studies
- Fish biology, ecology, and behavior
- Plant Virus Research Studies
- Immune cells in cancer
- Circular RNAs in diseases
- Cancer Genomics and Diagnostics
- Ion channel regulation and function
IRCCS Humanitas Research Hospital
2023-2024
Instituto di Biofisica
2019-2023
University of Milan
2014-2023
FuelCell Energy (United States)
2022
National Research Council
2020-2022
Ospedale Papa Giovanni XXIII
2021
Mylan (South Africa)
2020
Sorbonne Université
2014
Istituto Nazionale di Fisica Nucleare, Sezione di Torino
2014
University of Turin
2014
Abstract Artificial intelligence (AI)–powered approaches are becoming increasingly used as histopathologic tools to extract subvisual features and improve diagnostic workflows. On the other hand, hi-plex widely adopted analyze immune ecosystem in tumor specimens. Here, we aimed at combining AI-aided histopathology imaging mass cytometry (IMC) of non–small cell lung cancer (NSCLC). An AI-based approach was on hematoxylin eosin (H&E) sections from 158 NSCLC specimens accurately identify...
Tumor microenvironments are often characterized by an increase in oxidative stress levels. We studied the response to stimulation human primary (IGR39) or metastatic (IGR37) cell lines obtained from same patient, performing patch-clamp recordings, intracellular calcium ([Ca2+]i) imaging, and RT-qPCR gene expression analysis. In IGR39 cells, chloramine-T (Chl-T) activated large K+ currents (KROS) that were partially sensitive tetraethylammonium (TEA). A fraction of KROS was inhibited...
The tumor microenvironment (TME) is a complex ecosystem where malignant and non-malignant cells cooperate interact determining cancer progression. Cell abundance, phenotype localization within the TME vary over development in response to therapeutic interventions. Therefore, increasing our knowledge of spatiotemporal changes architecture importance better understand etiologic neoplastic diseases. Imaging Mass Cytometry (IMC) represents elective multiplexed imaging technology enabling in-situ...
While almost all animals are able to at least partially replace some lost parts, regeneration abilities vary considerably across species. Here we study gene expression patterns in distantly related species investigate conserved strategies. To this end, collect from the literature transcriptomic data obtained during of three (Hydra magnipapillata, Schmidtea mediterranea, and Apostichopus japonicus), compare them with vertebrates mammals. This allows us identify a common set differentially...
There is still no successful strategy to treat Huntington's disease, an inherited autosomal disorder associated with the aggregation of mutated forms huntingtin protein containing polyglutamine tracts more than 36 repeats. Recent experimental evidence challenging conventional view disease by revealing transcellular transfer proteins which are able seed oligomers involving wild type protein. Here we decipher molecular mechanism this unconventional heterogeneous oligomerization performing...
Approximately 20-50% of patients presenting with localized colorectal cancer progress to stage IV metastatic disease (mCRC) following initial treatment and this is a major prognostic determinant. Here, we have interrogated heterogeneous set primary (CRC), liver CRC metastases adjacent tissue identify molecular determinants the colon spreading. Screening Food Drug Administration (FDA) approved drugs for their ability interfere an identified metastasis signature may help filling unmet...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers. PDAC characterized by a complex tumor microenvironment (TME), that plays pivotal role in disease progression and resistance to therapy. Investigating spatial distribution interaction TME cells with basis for understanding mechanisms underlying represents current challenge research. Imaging mass cytometry (IMC) major multiplex imaging technology analysis heterogeneity. However, there dearth reports multiplexed IMC...
Melanoma is one of the most aggressive and highly resistant tumors. Cell plasticity in melanoma main culprits behind its metastatic capabilities. The detailed molecular mechanisms controlling are still not completely understood. Here we combine mathematical models phenotypic switching with experiments on IgR39 human cells to identify possible key targets impair switching. Our model shows that a cancer stem cell subpopulation within tumor prevents other cells. Experiments reveal hsa-mir-222...
Abstract Quantifying synergistic environmental effects in water contamination is still an open issue. Here, we have analyzed geolocalized data of pollutants recorded 2018 surface and groundwater Lombardy, one the areas with highest agricultural production rates, not only Italy, but also Europe. Both herbicides insecticides are present at concentration levels above legal limit, mainly waters. Geolocalized analysis allows us to identify interesting particularly affected by a combination...
The ability of bats to coexist with viruses without being harmed is an interesting issue that still under investigation. Here we use a mathematical model show the pattern body temperature variations observed in between day and night responsible for their keep check. From dynamical systems point view, our displays intriguing quasi-periodic behaviour might be relevant making system robust by avoiding viral escape due perturbations cycle.
<h3>Background</h3> General controlled nonderepressible 2 (GCN2) is a central kinase in the integrated stress response (ISR) that responds to amino acid deprivation. Cancer cells can utilize ISR for survival, but prolonged or hyperactivation of reduces proliferation and induces apoptosis. We are developing HC-7366, First-in-Class, First-in-Human GCN2 modulator activates GCN2, resulting anti-tumor activity. currently phase 1 trial (NCT05121948), has demonstrated robust efficacy multiple...
ABSTRACT The choanoflagellate Salpingoeca rosetta can differentiate into at least five morphologically and behaviorally distinct cell types during its lifetime, going from individual motile cells to linear rosette-shaped colonies. Due the capability form colonies close relationship with Metazoa, this organism is considered a model for studying multicellular evolution. gene regulatory programs underlying these transformations are, however, unknown. Here we analyze transcriptomic data obtained...
A delicate balance of reactive oxygen species (ROS) exists inside the cell: when mechanisms that control level ROS fail, cell is in an oxidative stress state, a condition can accelerate aging processes. To contrast pro-aging effect ROS, supplementation antioxidants has been recently proposed. Sulforaphane (SFN) isothiocyanate isolated from Brassica plants shown to modulate many critical factors cells helping counteract In present work, we exposed human dermal fibroblast short, sublethal and...
Abstract Background. While multiple studies have demonstrated improved clinical outcomes in patients receiving concurrent chemotherapy and immunotherapy, synergy may be limited by the destructive effects of DNA-damaging on non-cancer cells, including key constituents anti-tumor immunity (e.g. mature T cells). Furthermore, when first-line fails, subsequent lines produce lower response rates. Thus, new approaches that eradicate cancer cells while preserving immune required to more efficiently...
Abstract Background. Neutrophil elastase (ELANE) - a neutrophil-derived serine protease kills wide range of cancer cells without harming non-cancer through unique killing program involving histone H1 and the death domain CD95. Although ELANE attenuates tumor growth induces anti-tumor immunity following intratumoral delivery (Cui et al., Cell, 2021), systemic is stymied by presence inhibitors in blood (eg. alpha-1-antitrypsin (A1AT) which rapidly inhibit its enzymatic activity essential for...
<div>Abstract<p>Artificial intelligence (AI)–powered approaches are becoming increasingly used as histopathologic tools to extract subvisual features and improve diagnostic workflows. On the other hand, hi-plex widely adopted analyze immune ecosystem in tumor specimens. Here, we aimed at combining AI-aided histopathology imaging mass cytometry (IMC) of non–small cell lung cancer (NSCLC). An AI-based approach was on hematoxylin eosin (H&E) sections from 158 NSCLC specimens...
<p>Supplementary Figure 2</p>
<p>Supplementary Figure 1</p>