A5051046532- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Ion Channels and Receptors
- Migraine and Headache Studies
- Chronic Myeloid Leukemia Treatments
- Neuroblastoma Research and Treatments
- Cancer-related gene regulation
- Neuroscience of respiration and sleep
- Hemoglobinopathies and Related Disorders
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Neurological Complications and Syndromes
- Neuroscience and Neural Engineering
- Cancer therapeutics and mechanisms
- Protein Degradation and Inhibitors
- Microbial Inactivation Methods
- Protease and Inhibitor Mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Kruppel-like factors research
- Antimicrobial Peptides and Activities
National Research Council
2018-2024
Instituto di Biofisica
2012-2024
Institute of Genetics and Biophysics
2023
Ospedale Policlinico San Martino
2016-2021
University of Genoa
1989-2020
National Academies of Sciences, Engineering, and Medicine
2010-2011
Czech Academy of Sciences, Institute of Biophysics
2011
Universidade de São Paulo
2005
University of Palermo
1994
University of Ferrara
1984-1986
Cortical spreading depression (CSD), a wave of depolarization followed by cortical activity, is pathophysiological process implicated in migraine with aura and various other brain pathologies, such as ischemic stroke traumatic injury. To gain insight into the pathophysiology CSD, we generated mouse model for severe monogenic subtype aura, familial hemiplegic type 3 (FHM3). FHM3 caused mutations SCN1A, encoding voltage-gated Na+ channel NaV1.1 predominantly expressed inhibitory interneurons....
Tumor microenvironments are often characterized by an increase in oxidative stress levels. We studied the response to stimulation human primary (IGR39) or metastatic (IGR37) cell lines obtained from same patient, performing patch-clamp recordings, intracellular calcium ([Ca2+]i) imaging, and RT-qPCR gene expression analysis. In IGR39 cells, chloramine-T (Chl-T) activated large K+ currents (KROS) that were partially sensitive tetraethylammonium (TEA). A fraction of KROS was inhibited...
Background and purpose The volume regulated anion channel (VRAC) is known to be involved in different aspects of cancer cell behaviour response therapies. For this reason, we investigated the effect DCPIB, a presumably specific blocker VRAC, two types cancer: pancreatic duct adenocarcinoma (PDAC) melanoma. Experimental approach We used patch‐clamp electrophysiology, supported by Ca 2+ imaging, gene expression analysis, docking simulation mutagenesis. employed PDAC lines (Panc‐1 MiaPaCa‐2),...
The presynaptic P2X 7 receptor (P2X R) plays an important role in the modulation of transmitter release. We recently demonstrated that, nerve terminals adult rat cerebral cortex, R activation induced Ca 2+ ‐dependent vesicular glutamate release and significant ‐independent efflux through itself. In present study, we investigated effect new selective competitive antagonist 3‐(5‐(2,3‐dichlorophenyl)‐1H‐tetrazol‐1‐yl)methyl pyridine (A‐438079) on cerebrocortical terminal intracellular calcium...
Familial hemiplegic migraine 3 is an autosomal dominant headache disorder associated with aura and transient hemiparesis, caused by mutations of the neuronal voltage-gated sodium channel Nav1.1. While a gain-of function phenotype generally assumed to underlie familial migraine, this has not been fully explored. Indeed, major obstacle in studying vitro channels difficulty propagating mutagenizing expression plasmids containing their cDNAs. The aim work was study functional effect two...
Familial hemiplegic migraine (FHM) is a group of genetic migraine, associated with hemiparesis and aura. Three causative different genes have been identified, all which are involved in membrane ion transport. Among these, SCN1A encodes the voltage-gated Na+ channel Nav1.1, FHM caused by mutations named FHM3. For 7 12 known FHM3-causing SCNA1 functional consequences investigated, even if gain function effect seems to be predominant phenotype, for several conflicting results obtained available...
Adult neurogenesis is emerging as an important player in brain functions and homeostasis, while impaired or altered adult has been associated with a number of neuropsychiatric diseases, such depression epilepsy. Here we investigated the possibility that synapsins (Syns) I II, beyond their known developing mature neurons, also play role neurogenesis. We performed systematic evaluation distinct stages hippocampal dentate gyrus Syn II knockout (KO) mice, before (2-months-old) after...
Many studies highlighted the importance of IK channel for proliferation and migration different types cancer cells, showing how blockers could slow down growth. Based on these data, we wanted to characterize effects melanoma metastatic cells understand if such were exclusively IK-dependent. For this purpose, employed two blockers, namely clotrimazole senicapoc, cell lines: WM266-4 pancreatic Panc-1, which is reported have little or no expression. Clotrimazole senicapoc induced a decrease in...
Nanoplastics (NPs) represent an escalating hazard to both humans and the ecosystem due their pervasive presence. This review delves into (i) widespread occurrence of NPs across different environmental matrices, including food; (ii) routes estimates for human exposure; (iii) mechanisms blood–brain barrier (BBB) crossing; (iv) implications health, with a specific focus on molecular features associated neurotoxicity neurodegenerative processes. The impact central nervous system, ability cross...
Summary Activation of the oxidative burst and failure CD4+CD25+ cell regulation have been implicated in idiopathic nephrotic syndrome (iNS). The intimate mechanism is, however, unknown requires specifically focused studies. We investigated reactive oxygen species (ROS) generation [di-chlorofluorescein-diacetate (DCFDA)] fluorescence assay regulatory adenosine 5′-triphosphate (ATP) pathways blood 41 children with iNS, utilizing several agonists antagonists nucleotide/nucleoside receptors,...
Potassium channels have emerged as regulators of carcinogenesis, thus introducing possible new therapeutic strategies in the fight against cancer. In particular, large-conductance Ca2+-activated K+ channel, often referred to BK is involved several cancer-associated processes. Here, we investigated effects different activators, NS-11021, NS-19504, and BMS-191011, IGR39 (primary melanoma cell line) Panc-1 pancreatic duct carcinoma line), highly expressing IGR37 (metastatic that barely express...
Voltage-dependent sodium channels are membrane proteins essential for cell excitability. They composed by a pore-forming α-subunit and one or more β subunits. Nine α subunit five isoforms have been identified in mammals: β1, its splice variant β1B, β2, β3 β4. Although they do not form the ion channel pore, subunits modulate both function as well expression of on membrane.To investigate role β1 modulation expression, we silenced this auxiliary with specific antisense oligonucleotides (ASONs)...
In recent years, we have discovered Esc(1-21) and its diastereomer (Esc peptides) as valuable candidates for the treatment of Pseudomonas lung infection, especially in patients with cystic fibrosis (CF). Furthermore, engineered poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) were revealed to be a promising pulmonary delivery system antimicrobial peptides. However, “ad hoc” development novel therapeutics requires consideration their stability, tolerability, safety. Hence, by means...
The nine-member CLC gene family of Cl
The P2X(7) receptor (P2X(7)R) is an ATP-gated cation channel whose biophysical properties remain to be unravelled unequivocally. Its activity modulated by divalent cations and organic messengers such as arachidonic acid (AA). In this study, we analysed the differential modulation of magnesium (Mg(2+)) AA on P2X(7)R measuring whole-cell currents intracellular Ca(2+) ([Ca(2+)](i)) Na(+) ([Na(+)](i)) dynamics in HEK293 cells stably expressing full-length endowed with variant lacking entire...
Abstract DNA methylation at the 5′‐CCGG‐3′ sites of HLA‐DRα gene and relative flanking regions has been analyzed by Msp I Hpa II enzymatic digestion in order to determine whether a correlation exists between transcription gene. Unexpectedly, contrast behavior most eukaryotic genes, no positive was found hypomethylation expression. In fact, appears be fully unmethylated I/Hpa K562 cells, not expressing DR molecules, exhibit high degree Colo 38 which actively transcribe The unorthodox is...
To overcome the lack of effective pharmacological treatments for high-risk neuroblastoma (HR-NB), development novel in vitro and vivo models that better recapitulate disease is required. Here, we used an multiclonal cell model encompassing NB differentiation stages, to identify potential targets. This allowed us identify, by low-density RT-PCR arrays, two gene sets, one over-expressed during differentiation, other up-regulated more malignant cells. Challenging HR-NB expression datasets,...