A5016079998- Metabolism and Genetic Disorders
- Muscle metabolism and nutrition
- Neuroblastoma Research and Treatments
- Cancer therapeutics and mechanisms
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Research Studies
- Diet and metabolism studies
- Biochemical and Molecular Research
- Congenital heart defects research
- Congenital Heart Disease Studies
- Glycogen Storage Diseases and Myoclonus
- Signaling Pathways in Disease
- Connexins and lens biology
- Advanced Drug Delivery Systems
- Plant and animal studies
- Thermoregulation and physiological responses
- Cardiac Structural Anomalies and Repair
- GABA and Rice Research
- Biochemical Acid Research Studies
- 3D Printing in Biomedical Research
- Saffron Plant Research Studies
- Thermal Regulation in Medicine
- Cannabis and Cannabinoid Research
- Neuroscience and Neural Engineering
University of Genoa
2011-2025
National Research Council
2016-2024
Ospedale Policlinico San Martino
2016-2021
High Risk Neuroblastoma (HR-NB) is a pediatric cancer characterized by high malignancy and remarkable cell heterogeneity within the tumour nodules. In recent study, we demonstrated that in vitro vivo over-expression of non-coding RNA NDM29 (neuroblastoma differentiation marker 29) induces NB differentiation, dramatically reducing their malignancy. Among gene expression changes, differentiated phenotype induced decrease ABC transporters responsible for anticancer drug resistance. Thus,...
Aethina tumida, commonly known as the small hive beetle, poses a threat to honeybee populations, particularly Apis mellifera, across several European regions. Originating in sub-Saharan Africa, there is risk of infestation spreading from Calabria, region south Italy. The essential role played by spp. pollination biodiversity preservation, agricultural productivity, and overall economy related dangers posed invasion tumida. Current detection methods often fail identify infestations early,...
Cardiomyocyte renewal represents an unmet clinical need for cardiac regeneration. Stem cell paracrine therapy has attracted increasing attention to resurge rescue mechanisms within the heart. We previously characterized effects that human amniotic fluid–derived stem cells (hAFSC) can exert provide cardioprotection and enhance repair in preclinical models of myocardial ischemia cardiotoxicity. Here, we analyze whether hAFSC secretome formulations, namely, conditioned medium (hAFSC-CM) over...
The aim of this study was to compare the micro‐morphological features two different non‐drug Cannabis sativa L. biotypes (Chinese accession G‐309 and one fibrante variety) evaluate phytochemical profile as well some biological properties essential oils (EOs) obtained by hydrodistillation dried flowering tops. After a evaluation scanning electron microscopy, composition analysed GC–FID GC–MS analyses. Antioxidant anti‐acetylcholinesterase were investigated several in vitro cell‐free assays,...
In in vitro mouse hippocampal slices we investigated whether cyclocreatine is capable of entering brain cells independently the creatine transporter and if it reproduces neuroprotective effect creatine. Our study shows that does not increase content, but taken up as such then phosphorylated to phosphocyclocreatine. This uptake largely blocked by inactivation transporter, however some posphorylated even after inactivation. Thus, sets a cyclocreatine/phosphocyclocreatine system transporter....
Creatine, a compound that is critical for energy metabolism of nervous cells, crosses the blood-brain barrier (BBB) and neuronal plasma membrane with difficulty, only using its specific transporter. In hereditary condition where creatine transporter defective (creatine deficiency) there no in brain, administration useless lacking The disease severe incurable. Creatine-derived molecules could cross BBB independently might be useful to cure this condition. Moreover, such also stroke other...
To overcome the lack of effective pharmacological treatments for high-risk neuroblastoma (HR-NB), development novel in vitro and vivo models that better recapitulate disease is required. Here, we used an multiclonal cell model encompassing NB differentiation stages, to identify potential targets. This allowed us identify, by low-density RT-PCR arrays, two gene sets, one over-expressed during differentiation, other up-regulated more malignant cells. Challenging HR-NB expression datasets,...
Despite significant improvement of neuroblastoma (NB) patients’ survival due to recent treatment advancements in years, NB is still associated with high mortality rate. In search novel strategies increase NB’s susceptibility pharmacological treatments, we investigated the vitro and vivo effects fendiline hydrochloride as an enhancer cisplatin antitumor activity. To assess modulation on responses, used (evaluating cell proliferation by XCELLigence technology colony formation, gene expression...
High-risk neuroblastoma (HR-NB) still remains the most dangerous tumor in early childhood. For this reason, identification of new therapeutic approaches is fundamental importance. Recently, we combined conventional pharmacological approach to NB, represented by cisplatin, with fendiline hydrochloride, an inhibitor several transporters involved multidrug resistance cancer cells, which demonstrated enhancement ability cisplatin induce apoptosis. In work, co-administrated acetazolamide, a...
Several enzymes are involved in the energy production, becoming a possible target for new anti-cancer drugs. In this paper, we used biochemical and silico studies to evaluate effects of two guanidine molecules, (Boc)2-creatine metformin, on creatine kinase, an enzyme regulation intracellular levels. Our results show that both drugs inhibit kinase activity; however, displays competitive inhibition, while metformin acts with non-competitive mechanism. Moreover, is able activity hexokinase...
Background: Adenosine triphosphate (ATP) is the energy currency of body; it takes part in various and indispensable metabolic processes for maintenance cell homeostasis, degrading to its hydrolysis product, adenosine diphosphate (ADP). Efficient ways restore ATP are therefore necessary cells. When lacks due ischemic conditions or high demand, phosphocreatine gives phosphate group ADP that converts ATP, a reaction catalyzed by enzyme creatine kinase. For this reason, utilized as...