A5072578999- Extracellular vesicles in disease
- Galectins and Cancer Biology
- Advanced Proteomics Techniques and Applications
- Asthma and respiratory diseases
- Prostate Cancer Treatment and Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Mesenchymal stem cell research
- Plant-Microbe Interactions and Immunity
- Caveolin-1 and cellular processes
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Peptidase Inhibition and Analysis
- Neurological Disease Mechanisms and Treatments
- Eosinophilic Esophagitis
- Legume Nitrogen Fixing Symbiosis
- Microbial Natural Products and Biosynthesis
- Amyloidosis: Diagnosis, Treatment, Outcomes
- RNA modifications and cancer
- Biomarkers in Disease Mechanisms
- S100 Proteins and Annexins
- Ginkgo biloba and Cashew Applications
- IL-33, ST2, and ILC Pathways
- Cancer, Lipids, and Metabolism
- Plant nutrient uptake and metabolism
- Parkinson's Disease Mechanisms and Treatments
National Research Council
2019-2025
Institute of Biomedical Technologies
2013-2025
University of Parma
1998-2024
Istituto per le Tecnologie Didattiche
2024
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2019
China National Rice Research Institute
2019
AgriBio
2019
Jena University Hospital
2019
Agricultural Genomics Institute at Shenzhen
2019
Chinese Academy of Agricultural Sciences
2019
In this paper, a pilot production process for mesenchymal stem/stromal freeze-dried secretome was performed in validated good manufacturing practice (GMP)-compliant cell factory. Secretome purified from culture supernatants by ultrafiltration, added to cryoprotectant, lyophilized and characterized. We obtained freeze-dried, "ready-off-the-shelf" free soluble powder containing extracellular vesicles proteins. the product, not-aggregated population of detected nanoparticle tracking analysis;...
Asthma is a chronic inflammatory disease.Reticular basement membrane (RBM) thickening considered feature of airway remodelling (AR) particularly in severe asthma (SA).Omalizumab, mAb to IgE effective SA and can modulate AR.Herein we describe protein profiles bronchial biopsies detect biomarkers anti-IgE effects on AR explain potential mechanisms/pathways.We defined the biopsy profiles, before after treatment.Unsupervised clustering baseline proteomes resulted very good agreement with...
Bronchial asthma is a heterogeneous disease characterized by three cardinal features: chronic inflammation, variable airflow obstruction, and airway hyperresponsiveness. Asthma has traditionally been defined using nonspecific clinical physiologic variables that encompass multiple phenotypes are treated with anti-inflammatory therapies. Based on the modulation of remodeling after 12 months anti-immunoglobulin E (IgE) treatment, we identified two (omalizumab responder, OR; non-omalizumab NOR)...
Accumulating evidence shows that Mesenchymal Stem/Stromal Cells (MSCs) exert their therapeutic effects by the release of secretome, made both soluble proteins and nano/microstructured extracellular vesicles (EVs). In this work, for first time, we proved a proteomic investigation adipose-derived (AD)-MSC-secretome contains alpha-1-antitrypsin (AAT), main elastase inhibitor in lung, 72 other involved protease/antiprotease balance, 46 response to bacteria. By secretome fractionation, AAT is...
Background: Prostate cancer (PCa) is the second most prevalent solid tumor among men globally and leading non-skin in American men. In 2020 alone, approximately 1.4 million new cases of prostate were diagnosed worldwide, representing 14.1% all This multifactorial disease exhibits substantial variation incidence mortality across different ethnic groups geographic regions. Although prostate-specific antigen (PSA) remains widely used as a biomarker for PCa, its limitations reduce effectiveness...
Background: Prostate cancer (PCa), is the second most prevalent solid tumor among men worldwide (7.3%), and leading non-skin in USA where it represents 14.9% of all new cases diagnosed 2024. This multifactorial disease exhibits substantial variation incidence mortality across different ethnic groups geographic regions. Although prostate-specific antigen (PSA) remains widely used as a biomarker for PCa, its limitations reduce effectiveness accurate detection. Consequently, finding molecules...
Boron neutron capture therapy (BNCT) is a binary cancer treatment modality where two different agents (10B and thermal neutrons) have to be present produce an effect. A dedicated trial design necessary for early clinical trials. The concentration of 10B in tissues accepted surrogate predict BNCT effects on tissues. Tissue, blood, urines were sampled after infusion boron carriers, namely BSH BPA the frame European Organisation Research Treatment Cancer (EORTC) 11001. In this study, urine...
α-Synuclein (AS) is an intrinsically disordered protein highly expressed in dopaminergic neurons. Its amyloid aggregates are the major component of Lewy bodies, a hallmark Parkinson's disease (PD). AS particularly exposed to oxidation its methionine residues, both vivo and vitro. Oxidative stress has been implicated PD oxidized α-synuclein shown assemble into soluble, toxic oligomers, rather than fibrils. However, structural effects still poorly understood. In this work, was obtained by...
Plant mitoviruses belong to Mitoviridae family and consist of positive single-stranded RNA genomes replicating exclusively in host mitochondria. We previously reported the biological characterization a plant mitovirus, designated Chenopodium quinoa mitovirus 1 (CqMV1), some accessions. In this study, we analyzed mitochondrial proteome from leaves quinoa, infected not by CqMV1. Furthermore, protein-protein interaction co-expression network models, provided system perspective how CqMV1 affects...
Ex vivo lung perfusion (EVLP) is an emerging procedure that allows organ preservation, assessment and reconditioning, increasing the number of marginal donor lungs for transplantation. However, physiological airflow measurements are unable to unveil molecular mechanisms responsible EVLP beneficial effects on graft monitor proper course treatment. Thus, it urgent find specific biomarkers possess these requirements but also accurate reliable techniques identify them. The purpose this study...
We previously reported that c-KIT+ human amniotic-fluid derived stem cells obtained from leftover samples of routine II trimester prenatal diagnosis (fetal hAFS) are endowed with regenerative paracrine potential driving pro-survival, anti-fibrotic and proliferative effects. hAFS may also be isolated III clinical waste during scheduled C-sections (perinatal hAFS), thus offering a more easily accessible alternative when compared to fetal hAFS. Nonetheless, little is known about the profile...
Background: Extended half-life (EHL) factor IX (FIX) concentrates allow for prophylaxis with prolonged dosing intervals and high bleeding protection in persons hemophilia B. Long-term real-world studies are lacking. Methods: In a retrospective-prospective study, the six-year use of EHL recombinant FIX-albumin fusion protein (rIX-FP) was analyzed, comparing outcomes previous standard (SHL) FIX patients already on prophylaxis. Results: Prophylaxis rIX-FP prescribed 15 (10 severe, 5 moderate;...
Patients with severe allergic asthma (SAA) when treated omalizumab may exhibit different extent of response. Identifying biomarkers that can predict the treatment effectiveness in patients be useful personalizing treatment.Patients from longitudinal phase PROXIMA study were selected for this ancillary study. After 12 months treatment, categorized according to their response as: "clinical responder" (Asthma Control Questionnaire [ACQ] total score <1 at Month and/or a reduction number...
Pentraxin 3 (PTX3) is a main component of innate immune system by inducing complement pathway activation, acting as inflammatory mediator, coordinating the functions macrophages/dendritic cells and promoting apoptosis/necrosis. Additionally, it has been found in fibrotic regions co-localizing with collagen. In this work we wanted to investigate predictive role PTX3 myocardial damage fibrosis Duchenne Muscular Dystrophy (DMD). DMD an X-linked recessive disease caused mutations dystrophin gene...
Upon high throughput screening of 6700 microbial fermentation extracts, we discovered a compound, designated orthoformimycin, capable inhibiting protein synthesis in vitro with efficiency. The molecule, whose structure was elucidated by chemical, spectrometric, and spectroscopic methods, contains an unusual orthoformate moiety (hence the name) belongs to novel class translation inhibitors. This antibiotic does not affect any function 30S ribosomal subunit but binds 50S causing inhibition...
Cardiomyocyte renewal represents an unmet clinical need for cardiac regeneration. Stem cell paracrine therapy has attracted increasing attention to resurge rescue mechanisms within the heart. We previously characterized effects that human amniotic fluid–derived stem cells (hAFSC) can exert provide cardioprotection and enhance repair in preclinical models of myocardial ischemia cardiotoxicity. Here, we analyze whether hAFSC secretome formulations, namely, conditioned medium (hAFSC-CM) over...
The nucleocapsid protein Np of SARS-CoV-2 is involved in the replication, transcription, and packaging viral genome, but it also plays a role modulation host cell innate immunity inflammation response. Ectopic expression alone was able to induce significant changes proteome human cells. cellular RNA helicase DDX1 among proteins whose levels were increased by expression. its related DDX3X found physically interact with increase 2- 4-fold affinity for double-stranded helicase-independent...