A5020144250- Sustainable Supply Chain Management
- Product Development and Customization
- Sustainable Industrial Ecology
- Recycling and Waste Management Techniques
- Mesenchymal stem cell research
- Silk-based biomaterials and applications
- Environmental Impact and Sustainability
- Service and Product Innovation
- Digital Transformation in Industry
- Manufacturing Process and Optimization
- Bone Tissue Engineering Materials
- Design Education and Practice
- Extracellular vesicles in disease
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Flexible and Reconfigurable Manufacturing Systems
- Quality and Supply Management
- Graphene and Nanomaterials Applications
- 3D Printing in Biomedical Research
- Digital Platforms and Economics
- Environmental Sustainability in Business
- Sharing Economy and Platforms
- Technology Assessment and Management
- Wound Healing and Treatments
- Antimicrobial Peptides and Activities
University of Applied Sciences and Arts of Southern Switzerland
2014-2024
Technology Transfer System (Italy)
2018
National Academies of Sciences, Engineering, and Medicine
2006
In this paper, a pilot production process for mesenchymal stem/stromal freeze-dried secretome was performed in validated good manufacturing practice (GMP)-compliant cell factory. Secretome purified from culture supernatants by ultrafiltration, added to cryoprotectant, lyophilized and characterized. We obtained freeze-dried, "ready-off-the-shelf" free soluble powder containing extracellular vesicles proteins. the product, not-aggregated population of detected nanoparticle tracking analysis;...
To validate the use of ultrafiltration (UF) as an alternative applicable industrial method to replace ultracentrifugation (UC) in purification mesenchymal stromal cell (MSC)-secretome.Pharmaceutical formulations containing secretome and/or extracellular vesicles were extracted from adipose-MSCs and bone marrow-MSCs by combining UF or UC with lyophilization.UF led higher protein, lipid, cytokine exosomes yields compared UC. The isolation procedure source influenced immunomodulatory activity,...
Over the past decades more and production companies have adopted lean paradigm to get operational performance improvements. More recently, competitive environment is requiring an increasing attention towards sustainability issues leading implementation of practices also improve and, in particular, environmental impacts. The relationships between green approaches terms their mutual influence achieving target been investigated several studies (see for example (Dieste et al., 2019)– (Henao...
Digitalization has shown the potential to disrupt industrial value chains by supporting real-time, risk-free and inexpensive inputs decision making towards enhanced companies' productivity networks flexibility. Developing a reliable robust digital replica of physical systems chain is one most advanced (and challenging) approaches digitalization, condensed in concept Digital Twin (DT). DT plays fundamental role creating data-rich environment where simulation optimization procedures can be...
Three-dimensional printing of poly(ε-caprolactone) (PCL) is a consolidated scaffold manufacturing technique for bone regenerative medicine. Simultaneously, the mesenchymal stem/stromal cell (MSC) secretome osteoinductive, promoting colonization by cells, proliferation, and differentiation. The present paper combines 3D-printed PCL scaffolds with lyosecretome, freeze-dried formulation MSC secretome, containing proteins extracellular vesicles (EVs). We designed lyosecretome two loading...
Arg-Gly-Asp (RGD)-based cyclopentapeptides (cRGDs) have a high affinity towards integrin αvβ3 and αvβ5, which are overexpressed by many tumor cells. Here, curcumin-loaded silk fibroin nanoparticles (SFNs) been functionalized on the surface with cRGD to provide active targeting cells; “click reaction” between RGD-based cyclopentapeptide carrying an azide group triple-bond-functionalized has exploited. Both naked SFNs were less than 200 nm in diameter showed round-shaped morphology but, after...
Abstract BACKGROUND In the last decade, many researchers demonstrated biological activities of native Bombyx mori silk sericin (SS), and its use is widespread in cosmetic biomedical field. However, SS a polluting material from fibroin textile industry. This paper aims to demonstrate that industrial wastewater‐derived has properties, can be used as an eco‐friendly product for cosmetic/pharmaceutical purposes with important impact on circular economy. RESULTS We focused derived ad hoc...
Producing mesenchymal stem cell (MSC)-secretome for dose escalation studies and clinical practice requires scalable good manufacturing (GMP)-compliant production procedures formulation into a standardized medicinal product. Starting from method that combines ultrafiltration freeze-drying to transform MSC-secretome pharmaceutical product, the lyosecretome, this work aims to: (i) optimize lyosecretome formulation; (ii) investigate sources of variability can affect robustness process; (iii)...
Recently, 3D-printed scaffolds for the controlled release of mesenchymal stem cell (MSC) freeze-dried secretome (Lyosecretome) have been proposed to enhance scaffold osteoinduction and osteoconduction; coprinting poly(ε-caprolactone) (PCL) with alginate hydrogels allows adequate mechanical strength be combined modulable kinetics active principle release. This study represents feasibility sterile production coprinted proof concept their in vitro biological efficacy. Sterile were obtained,...
Mesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed sustain regeneration by tissue-resident MSCs. SNPs prepared exploiting self-assembly method poloxamer and proanthocyanidins (P), quercetin (Q) or epigallocatechin gallate (E). SNPs, diameter less than 150...
SmartBone® (SB) is a biohybrid bone substitute advantageously proposed as class III medical device for regeneration in reconstructive surgeries (oral, maxillofacial, orthopedic, and oncology). In the present study, new strategy to improve SB osteoinductivity was developed. scaffolds were loaded with lyosecretome, freeze-dried formulation of mesenchymal stem cell (MSC)-secretome, containing proteins extracellular vesicles (EVs). Lyosecretome-loaded (SBlyo) prepared using an absorption method....
The come to power of the sharing economy paradigm has incentivized adoption service-based business models largely relying on digital platforms providing infrastructure and ecosystems sustain market uptake. manufacturing domain isn't exempted from this trend, witnessing growing launch with aim shifting value proposition towards a model product servitization share, instead ownership. Most current examples in are very narrowed-scope systems that just connect providers or, when giving detailed...
Silk fibroin nanoparticles (SFNs) have been widely investigated for drug delivery, but their clinical application still faces technical (large-scale and GMP-compliant manufacturing), economic (cost-effectiveness in comparison to other polymer-based nanoparticles), biological (biodistribution assessments) challenges. To address biodistribution challenge, we provide a straightforward desolvation method (in acetone) produce homogeneous SFNs incorporating increasing amounts of Fe
This article proposes a decision support system to improve the management of middle-of-life phase according product life cycle (LC) cost evaluation. A new maintenance approach integrates information gathered from different actors in order obtain data which may impact on whole value chain. specific algorithm calculating residual LC costs is used for optimisation interventions fleet trucks. The proposed enables create value-transforming into knowledge available all phases and meant...
Manufacturing companies continuously face the challenge of improving their sustainability profile, but one major problems with sustainability-centred business approaches relates to simultaneous pursuit private and public (society environment) benefits. Industrial symbiosis (IS) can be a powerful answer these approaches: IS lead virtuous behaviours coupling environmental (public) benefits economic (private) performances. projects opportunities are amplified for belonging industrial parks or...
Ageing, occurrence of work-related musculoskeletal disorders, and rising sensitivity towards the disabled are paving way for new, customized movement assisting devices enabling increased mobility independence, correction user's kinesis prevention diseases, combined with a constant monitoring kinematics conditions. This paper presents digital double manufacturing, delivery maintenance chain(s) developed those devices, focusing on its data model, logics, services optimization functionalities...
The sharing economy emerged in recent years as a model disrupting the approach to traditional B2B and B2C value chains by giving access underutilized resources at fraction of cost whom cannot or do not want buy new products. In this context, multi-sided platforms (MSPs) play pivotal role providing environments technological infrastructures able match make needs manifold user insisting on them. manufacturing sector didn't remain untouched trend, but still struggle set up drivers supporting...
SMEs rarely have a pre-defined pattern for the conceiving and development of innovative solutions. Most innovation management steps rely on designers' managers' intuition with no structured path to generating ideas. Especially in turbulent economic environments companies need efficiently choose most promising ideas order effectively allocate internal resources innovation. What is proposed this paper platform tools supporting managers different process, from identification company...