A5058208273- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Carbohydrate Chemistry and Synthesis
- Analytical Chemistry and Chromatography
- Tuberculosis Research and Epidemiology
- Silk-based biomaterials and applications
- vaccines and immunoinformatics approaches
- Transgenic Plants and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Proteomics Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Microbial Natural Products and Biosynthesis
- Microfluidic and Capillary Electrophoresis Applications
- Protein Hydrolysis and Bioactive Peptides
- 3D Printing in Biomedical Research
- Toxin Mechanisms and Immunotoxins
- Chemical Synthesis and Analysis
- Collagen: Extraction and Characterization
- Bee Products Chemical Analysis
- Vitamin D Research Studies
- Natural product bioactivities and synthesis
- Enzyme Production and Characterization
- Chronic Lymphocytic Leukemia Research
University of Pavia
2016-2025
Vrije Universiteit Amsterdam
2017
Sodium alginate (SA)-based hydrogels are often employed as bioink for three-dimensional (3D) scaffold bioprinting. They offer a suitable environment cell proliferation and differentiation during tissue regeneration also control the release of growth factors mesenchymal stem secretome, which is useful biointegration. However, such show poor mechanical properties, fast-release kinetics, low biological performance, hampering their successful clinical application. In this work, silk fibroin...
Hydrophilic interaction liquid chromatography (HILIC) has always been devoted to the analysis of small polar compounds, glycans and glycopeptides. However, in last 10 years HILIC emerged as an analytical tool for intact proteins, showing peculiar potentialities separation protein glycoprotein variants. The great compatibility with mass spectrometry detection its unique complementarity reverse phase makes interesting option characterization. In this review a comprehensive overview insight...
Glycosylation is considered a critical quality attribute of therapeutic proteins. Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position glycans. Whereas analysis released glycans glycopeptides provides information about composition and/or glycan, intact glycoprotein allows assignment individual proteoforms co-occurring modifications. Yet, resolving protein glycoforms at level challenging. We have explored capacity hydrophilic liquid...
Peroxisome proliferator-activated receptors (PPARs) belong to the superfamily of nuclear and represent targets for therapeutical treatment type 2 diabetes, dyslipidemia hyperglycemia associated with metabolic syndrome. Some medicinal plants have been traditionally used treat this kind diseases. Today only few drugs targetting PPARs approved reason, rapid identification novel ligands and/or chemical scaffolds starting from natural extracts would benefit a selective affinity ligand fishing...
In this study, an HPLC HILIC-UV method was developed for the analysis of intact neo-glycoproteins. During development experimental conditions evaluated involved different HILIC columns (TSKgel Amide-80 and ZIC-pHILIC), water-acetonitrile mixtures containing various types acids salts. The final selected based on a TSKgel column mobile phase composed acetonitrile water both 10 mM HClO4. influence temperature sample preparation chromatographic performances also investigated. applied to...
Arg-Gly-Asp (RGD)-based cyclopentapeptides (cRGDs) have a high affinity towards integrin αvβ3 and αvβ5, which are overexpressed by many tumor cells. Here, curcumin-loaded silk fibroin nanoparticles (SFNs) been functionalized on the surface with cRGD to provide active targeting cells; “click reaction” between RGD-based cyclopentapeptide carrying an azide group triple-bond-functionalized has exploited. Both naked SFNs were less than 200 nm in diameter showed round-shaped morphology but, after...
Abstract Background To create a dual-acting vaccine that can fight against tuberculosis, we combined antigenic arabino-mannan analogues with the Ag85B protein. start process, studied impact of modifying different parts protein on its ability to be recognized by antibodies. Results Through our research, discovered three modified versions protein, rAg85B-K30R, rAg85B-K282R, and rAg85B-K30R/K282R, retained their antibody reactivity in healthy individuals those tuberculosis. further test...
An efficient chemoenzymatic strategy followed by an integrated <italic>in silico</italic> and proteomic analysis for the preparation of neoglycoproteins was described.
Abstract Exogenous application of human epidermal growth factor (hEGF) stimulates wound healing. The aim this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, particular treating skin-related disorders as burns. Ribonuclease A (RNase A) used investigate a number different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose glyoxyl-agarose showed preserve appropriate orientation...
During collagen biosynthesis, proline is post-translationally converted to hydroxyproline by specific enzymes. This amino acid, unique collagen, plays a crucial role in stabilizing the triple helix structure and could serve as an important biomarker for content quality analysis. Hydroxyproline has four isomers, depending on whether hydroxylated at position 4 or 3 cis- trans- conformation formed. Moreover, extensive hydrolysis of required its acid analysis, epimerization may also occur,...
Tuberculosis is still one of the most deadly infectious diseases worldwide, and use conjugated antigens, obtained by combining antigenic oligosaccharides, such as lipoarabinomannane (LAM), with proteins from Mycobacterium tuberculosis (MTB), has been proposed a new strategy for developing efficient vaccines. In this work, we investigated effect chemical glycosylation on two recombinant MTB produced in E. coli an additional seven-amino acid tag (recombinant Ag85B TB10.4). Different...
Oligosaccharides are a key component on glycoconjugates with biomedical applications. However, the investigation of more efficient and environmental protocols for their preparation carrying defined oligosaccharides remains big challenge due to lack access structurally well-defined functionalized oligosaccharides. In this work highly sustainable chemoenzymatic synthesis neo-glycoproteins in green solvents was studied. particular, we described new activated disaccharides useful direct protein...
Tuberculosis is the deadliest infectious disease in world. The variable efficacy of current treatments highlights need for more effective agents against this disease. In past few years, we focused on investigation antigenic glycoconjugates starting from recombinant Ag85B (rAg85B), a potent protein antigen Mycobacterium tuberculosis. paper, structural modifications were rationally designed order to obtain rAg85B variant able maintain its immunogenicity after glycosylation. Lysine residues...