A5005199763- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Radiopharmaceutical Chemistry and Applications
- Advanced Proteomics Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Biosensing Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- HER2/EGFR in Cancer Research
- Nanoparticle-Based Drug Delivery
- Click Chemistry and Applications
- Ion-surface interactions and analysis
- Genetic Neurodegenerative Diseases
- Microfluidic and Bio-sensing Technologies
- Acute Lymphoblastic Leukemia research
- Advanced biosensing and bioanalysis techniques
- Plant and Biological Electrophysiology Studies
- Electrochemical sensors and biosensors
- Metabolomics and Mass Spectrometry Studies
- Field-Flow Fractionation Techniques
- Chromatography in Natural Products
- Chemical Synthesis and Analysis
Vrije Universiteit Amsterdam
2015-2024
University of Amsterdam
2020-2023
Stichting tot Steun VCVGZ
2021
University of Tasmania
2015
Utrecht University
2007-2014
Abilities In Motion
2012
University of Twente
2005
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCapillary Electrophoresis: Trends and Recent AdvancesRobert L. C. VoetenRobert VoetenDivision of BioAnalytical Chemistry, Department Chemistry Pharmaceutical Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1085, 1081 HV The NetherlandsTI-COAST, Science Park 904, 1098 XH NetherlandsMore by Robert VoetenView Biography, Iro K. VentouriIro VentouriTI-COAST, NetherlandsAnalytical Group, van't Hoff Institute for Molecular University VentouriView Rob...
Capillary electrophoresis coupled to time-of-flight mass spectrometry (CE–TOF-MS) via a porous tip sheathless electrospray ionization (ESI) interface was studied for the characterization of pharmaceutical glycoproteins. To achieve optimal glycoform separation, background electrolytes low pH were used in conjunction with capillary neutral coating exhibiting near-zero electroosmotic flow. Crucial interfacing parameters, like ESI voltage and tip-to-end plate distance, optimized very flow rates...
To compare the quality of therapeutic erythropoietin (EPO) products, including two biosimilars, with respect to content, aggregation, isoform profile and potency.Two original Eprex (epoetin alpha) Dynepo delta), biosimilar Binocrit Retacrit zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis (5) in-vivo potency.Tested EPO products differed in composition, potency.Of tested biosimilars have same or even...
In this work, the usefulness of capillary electrophoresis–electrospray ionization time-of-flight–mass spectrometry for analysis biopharmaceuticals was studied. Noncovalently bound coatings consisting Polybrene-poly(vinyl sulfonic acid) or Polybrene-dextran sulfate-Polybrene were used to minimize protein and peptide adsorption, achieve good separation efficiencies. The potential electrophoresis-mass (CE-MS) system characterize degradation products investigated by analyzing samples drugs,...
Glycosylation is considered a critical quality attribute of therapeutic proteins. Protein heterogeneity introduced by glycosylation includes differences in the nature, number and position glycans. Whereas analysis released glycans glycopeptides provides information about composition and/or glycan, intact glycoprotein allows assignment individual proteoforms co-occurring modifications. Yet, resolving protein glycoforms at level challenging. We have explored capacity hydrophilic liquid...
Antibody-based pharmaceuticals often encompass a complex structural heterogeneity requiring enhanced analytical methods for reliable characterization of variants and degradation products. We have explored the capabilities low-flow sheathless capillary electrophoresis-mass spectrometry (CE-MS) high-resolution sensitive profiling antibody therapeutics. Near-zero electroosmotic flow was achieved by employing novel neutral coating that also prevents protein adsorption. CE-MS analysis intact...
Size-exclusion chromatography employing aqueous mobile phases with volatile salts at neutral pH combined electrospray-ionization mass spectrometry (SEC-ESI-MS) is a useful tool to study proteins in their native state. However, whether the applied eluent conditions actually prevent protein–stationary phase interactions, and/or protein denaturation, often not assessed. In this study, effects of additives on SEC retention and ESI were thoroughly investigated. Myoglobin was used as main model...
Among all the –omics approaches, proteomics and metabolomics have received increased attention over last decade. Both approaches reached a certain level of maturity, showing their relevance in numerous clinical applications, including biomarkers discovery, improved diagnosis, staging, prognosis diseases, as well better knowledge on various (patho-)physiological processes. Analytically, reversed-phase liquid chromatography – mass spectrometry (RPLC-MS) is considered golden standard...
A flow-through microvial is used to interface capillary electrophoresis and mass spectrometry (CE-MS) develop a method for simultaneous profiling both neutral sialylated glycans without derivatization or labeling. The CE separation was performed at near-zero electroosmotic flow in with neutral, hydrophilic coating, using 50 mM ammonium acetate 20% methanol (pH 3.1) as the background electrolyte. optimized reversed polarity negative ion ESI-MS. Enzymatically released N-glycans from human...
Amyotrophic lateral sclerosis (ALS) is characterized by the progressive loss of motor neurons and premature death. The limited understanding mechanisms underlying selective neuron death has significantly hindered development disease-modifying treatments. Ferroptosis, a form cell dependent on iron accumulation, been implicated in degeneration ALS. Oxidized phosphatidylcholines (PC-OxPL) have identified as key effectors pathophysiological processes associated with this pathway. Preclinical...
The usefulness of a noncovalent, positively charged capillary coating for the efficient analysis intact basic proteins with CE was studied. Capillaries were coated by subsequent flushing solutions 10% w/v Polybrene (PB), 3% dextran sulfate (DS), and again PB. Coating characterization studies showed that stable coatings could be produced which exhibited pH-independent highly reproducible EOF. PB-DS-PB evaluated Tris phosphate BGEs various pH using four model proteins: alpha-chymotrypsinogen...
A collaborative study on the robustness and portability of a capillary electrophoresis‐mass spectrometry method for peptide mapping was performed by an international team, consisting 13 independent laboratories from academia industry. All participants used same batch samples, reagents coated capillaries to run their assays, whereas they utilized equipment available in laboratories. The varied model, type instrument manufacturer. Furthermore, different types sheath‐flow electrophoresis–mass...
Abstract Greater control is desirable in the stochastic conjugation technology used to synthesize antibody–drug conjugates (ADC). We have shown recently that a fluorescent dye can be stably conjugated mAb using bifunctional platinum(II) linker. Here, we describe general applicability of this novel linker for preparation stable and efficacious ADCs. The ethylenediamine moiety, herein called Lx, was coordinated Desferal (DFO) or auristatin F (AF) provide storable “semifinal” products, which...