A5073334841- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Selenium in Biological Systems
- Ion-surface interactions and analysis
- Pesticide Residue Analysis and Safety
- Advanced Chemical Sensor Technologies
- Isotope Analysis in Ecology
- Glycosylation and Glycoproteins Research
- Mercury impact and mitigation studies
- Nuclear Physics and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Chemical Synthesis and Analysis
- Trace Elements in Health
- Transgenic Plants and Applications
- Fermentation and Sensory Analysis
- Analytical Chemistry and Sensors
- Bacterial Identification and Susceptibility Testing
- Molecular Sensors and Ion Detection
- Lipid Membrane Structure and Behavior
- Plant biochemistry and biosynthesis
- Pharmacological Effects and Assays
- Medical Imaging Techniques and Applications
University of Liège
2016-2025
IONICS Mass Spectrometry (Canada)
2025
Agora
2016-2017
Islamic Azad University, Science and Research Branch
2015-2017
Laboratoire de Chimie
2009-2016
Element Six (Germany)
2016
Centre National de la Recherche Scientifique
2009-2012
Institut des Sciences Analytiques et de Physico-Chimie pour l'Environnement et les Matériaux
2009-2010
In-Q-Tel
2009
University of Zurich
1995
Per- and polyfluoroalkyl substances (PFAS) are contaminants of increasing concern, with over seven million compounds currently inventoried in the PubChem PFAS Tree. Recently, ion mobility spectrometry has been combined liquid chromatography high-resolution mass (LC-IMS-HRMS) to assess PFAS. Interestingly, using negative electrospray ionization, perfluoroalkyl carboxylic acids (PFCAs) form homodimers ([2M-H]-), a phenomenon observed trapped, traveling wave, drift-tube IMS. In addition limited...
Summary Background and Objective Due to the increasing popularity of exotic fruits in Western diet, allergologists are confronted with allergic reactions substances these plants. The present report describes an anaphylactic reaction after consumption lychee fruit ( Litchi sinensis ). atopic patient also suffers from rhinoconjunctivitis due a sensitization against pollen Compositae family as well dyspnoea eating sunflower seeds. Our goals were determine crossreactivity between antibodies...
An analytical approach allowing the identification of unknown seleniummetabolites in selenium-rich yeast was described. Anion-exchange HPLC Se-metabolome fraction co-eluting with salts size-exclusion chromatography allowed separation nine selenium species (excluding isomers and selenate) as monitored by inductively coupled plasma mass spectrometry (ICP MS). The individual fractions were analyzed electrospray QTOF MS/MS hybrid linear ion trap/OrbitrapMSn after sample introduction...
Ion mobility (IM) is now a well-established and fast analytical technique. The IM hardware constantly being improved, especially in terms of the resolving power. Drift Tube (DTIMS), Traveling Wave (TWIMS), Trapped Mobility Spectrometry (TIMS) coupled to mass spectrometry are used determine Collision Cross-Sections (CCS) ions. In chemistry, CCS approached as descriptor for ion identification it also physical chemistry 3D structure elucidation with computational support. extracted from reduced...
Rationale Mass spectrometry (MS) is the reference method for screening of ultra‐trace residues pesticides in food because MS offers required selectivity/sensitivity to gather information and enable analyst make informed decisions during identification process. Here we present discuss use collision cross section (CCS) values addition mass accuracy retention times a pesticide that integrates all features offered by coupling ultra‐performance liquid chromatography (UPLC) with ion mobility...
Kendrick mass defect (KMD) analysis is widely used for helping the detection and identification of chemically related compounds based on exact measurements. We report here use KMD as a criterion filtering complex spectrometry data set. The method allow automated, easy efficient processing, enabling reconstruction 2D distributions families homologous from MSI images. show that filtering, in-house software, suitable robust high resolution (full width at half-maximum, fwhm, m/z 410 20 000) very...
Strong cation exchange HPLC with the parallel ICP MS and electrospray hybrid linear ion trap quadrupole orbital mass spectrometry (ESI Orbitrap MS) detection was developed for study of metabolomic pattern selenium in selenium-rich yeast. The mobile phase composition (gradient ammonium formate 20% methanol) optimized to obtain separation conditions guaranteeing identical sensitivity during entire chromatographic run compatibility ionization. Twenty seven Se-containing metabolites observed...
A method was developed for the comprehensive cartography of selenium metabolites synthesized in process conversion selenite [Se(iv)] into organic compounds and enrichment yeast with selenium. The number detected considerably increased (49) owing to optimization fractionation procedure use UPLC. purity minor selenoorganic amino acids oligopeptides allowed successful on-line de-novo identification based on exact mass (<1 ppm) fragmentation (MS2/MS3) spectra obtained high resolution accuracy....
A collaborative study on the robustness and portability of a capillary electrophoresis‐mass spectrometry method for peptide mapping was performed by an international team, consisting 13 independent laboratories from academia industry. All participants used same batch samples, reagents coated capillaries to run their assays, whereas they utilized equipment available in laboratories. The varied model, type instrument manufacturer. Furthermore, different types sheath‐flow electrophoresis–mass...
We have probed for reaction intermediates involved in the dual-gold-catalyzed activation of a conjugated 1,5-diyne substrate and its further coupling to benzene liquid phase. This was done by sampling mixture electrospray ionization followed high-resolution ion mobility mass spectrometry—under conditions allowing resolution structural isomers differing their collision cross sections less than 0.5%. For cationic corresponding catalyst + diyne (activation stage) we resolve four isomers. At...
Ion mobility spectrometry (IMS) is a gas phase separation technique, which relies on differences in collision cross section (CCS) of ions. Ionic clouds unresolved conformers overlap if the CCS difference below instrumental resolution expressed as CCS/ΔCCS. The experimental arrival time distribution (ATD) peak then superimposition various contributions weighted by their relative intensities. This paper introduces strategy for accurate drift determination using traveling wave ion (TWIMS)...
MALDI mass spectrometry imaging (MALDI MSI) is a powerful analytical method for achieving 2D localization of compounds from thin sections typically but not exclusively biological samples. The dynamically harmonized ICR cell (ParaCell) was recently introduced to achieve extreme spectral resolution capable providing the isotopic fine structure ions detected in complex latest improvement technology also includes 2ω detection, which significantly reduces transient time while preserving nominal...
The current trend for increasing technical complexity in the field of CE-ESI-MS interfaces has incited more accessible alternatives. In this work, a simple low sheath-flow ESI interface operating submicroliter nanospray regime without nebulizing gas assistance was evaluated. use sheath liquid enabled improving ionization analytes, while absence minimized sample dilution and loss efficiency. After rapid qualification, effect main operational parameters such as composition flow rate, working...
Many studies have evidenced the main role of lipids in physiological and also pathological processes such as cancer, diabetes or neurodegenerative diseases. The identification situ localization specific low-abundant lipid species involved cancer biology are still challenging for both fundamental marker discovery. In this paper, we report isobaric minor phospholipids human breast xenografts by FTICR MALDI imaging supported histochemistry. These potential candidates can be further confirmed...
Over the years, polymer analyses using ion mobility-mass spectrometry (IM-MS) measurements have been performed on different mobility (IMS) setups. In order to be able compare literature data taken IM(-MS) instruments, heating and temperature evaluations already explored. Nevertheless, extrapolations other analytes are difficult thus straightforward same-sample instrument comparisons seem only reliable way make sure that setups do not greatly change gas-phase behavior. We used a large range...
Despite well-established pathways and metabolites involved in grapevine-Plasmopara viticola interaction, information on the molecules first moments of pathogen contact with leaf surface their specific location is still missing. To understand localise these molecules, we analysed grapevine discs infected P. MSI. Plant material preparation was optimised, different matrices solvents were tested. Our data shows that trichomes hamper matrix deposition ion signal. Results show putatively...
Summary Production of recombinant pharmaceutical glycoproteins has been carried out in multiple expression systems. However, N ‐glycosylation, which increases heterogeneity and raises safety concerns due to the presence non‐human residues, is usually not controlled. The composition ‐glycans are also susceptible affect protein stability, function immunogenicity. To tackle these issues, we developing glycoengineered Nicotiana tabacum Bright Yellow‐2 (BY‐2) cell lines through knock ectopic...
Ion mobility-mass spectrometry (IM-MS) has emerged as a powerful separation and identification tool to characterize synthetic polymer mixtures topologies (linear, cyclic, star-shaped,…). Electrospray coupled IM-MS already revealed the coexistence of several charge state-dependent conformations for single state biomolecules with strong intramolecular interactions, even when limited resolving power instruments were used. For polymers, sample's polydispersity allows observation chain lengths. A...
We used tandem ion mobility spectrometry measurements to investigate how the photo-isomerization of a chromophore (a methylpyridinium derivative) is affected by complexation with crown ether.
Electrospray ionization of [RuCl<sub>2</sub>(<italic>p</italic>-cymeme)(PTA)] afforded a mixture two molecular ions resulting from an in source oxidation Ru<sup>II</sup> into Ru<sup>III</sup> or protonation the 1,3,5-triaza-7-phosphaadamantane (PTA) ligand.