A5015405140- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Advanced Chemical Sensor Technologies
- Pesticide Residue Analysis and Safety
- Neuropeptides and Animal Physiology
- Pharmacological Effects and Assays
- Identification and Quantification in Food
- Analytical Chemistry and Chromatography
- Advanced biosensing and bioanalysis techniques
- Neurological Disorders and Treatments
- Ion-surface interactions and analysis
- SARS-CoV-2 and COVID-19 Research
- Meat and Animal Product Quality
- Advanced Proteomics Techniques and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Biomedical Research and Pathophysiology
- Spectroscopy and Chemometric Analyses
- Analytical Methods in Pharmaceuticals
- Flavonoids in Medical Research
- Plant biochemistry and biosynthesis
- Cholinesterase and Neurodegenerative Diseases
- Forensic Toxicology and Drug Analysis
- Lysosomal Storage Disorders Research
Waters (United States)
2011-2017
University of Antwerp
2016
Steklov Mathematical Institute
2010
Wythenshawe Hospital
1999
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, virus that causes COVID-19, spread so rapidly across globe. The policy response to pandemics will always have include accurate monitoring of spread, this provides one few alternatives total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by reverse transcription polymerase chain reaction (RT-PCR). Although efficient, automatable, acceptably cheap, reliance on...
Travelling wave ion mobility spectrometry – mass (TWIMS‐MS) was evaluated as a tool for structural identification of metabolites small molecule drugs in cases where the exact position biotransformation could not be identified by conventional tandem spectrometry. Test sets compounds containing biotransformations at aromatic positions were analyzed. These present problem traditional MS methods since an atomic level localization cannot normally determined from n spectra. In addition to...
Modern mass spectrometry methods provide a huge benefit to saponin structural characterization, especially when combined with collision-induced dissociation experiments obtain partial description of the (ion) structure. However, complete structures these ubiquitous secondary metabolites remain challenging, since isomeric saponins presenting small differences are often present in single extract. As typical example, horse chestnut triterpene glycosides, so-called escins, comprise containing...
Ion mobility-mass spectrometry (IM-MS) has emerged as a powerful separation and identification tool to characterize synthetic polymer mixtures topologies (linear, cyclic, star-shaped,…). Electrospray coupled IM-MS already revealed the coexistence of several charge state-dependent conformations for single state biomolecules with strong intramolecular interactions, even when limited resolving power instruments were used. For polymers, sample's polydispersity allows observation chain lengths. A...
Abstract In this study a new method for the simultaneous confirmation of betamethasone and dexamethasone residues in bovine liver is presented. A Quattro LCZ triple quadrupole mass spectrometer, equipped with an atmospheric pressure ionization (API) source, was coupled to high performance liquid chromatograph (HPLC) system. Spiked samples were first extracted acetonitrile, extracts purified on C‐18 columns. LC separations performed Hypercarb column, acetonitrile/water (90:10, v/v, +0.3%...
Cerebrolysin, a parenteral peptide preparation produced by controlled digestion of porcine brain proteins, is an approved nootropic medicine in some countries. However, it also easily and globally available on the Internet. Nevertheless, until now, its exact chemical composition was unknown. Using high performance liquid chromatography (HPLC) coupled to ion trap ultra (UHPLC) quadrupole‐ion mobility‐time‐of‐flight mass spectrometry (Q‐IM‐TOF MS), combined with UniProt pig protein database...
A novel, sensitive, high performance liquid chromatography/tandem mass spectrometric (i.e. spectrometry/mass spectrometry) method with on-line extraction and clean-up for the screening confirmation of residues tetracyclines in kidney has been developed. After homogenised McIlvain buffer, an aliquot extract is directly injected on LC/MS/MS system further sample on-line. Detection analytes was achieved by positive electrospray ionization followed multiple reaction monitoring. For each...
Aim: An ultrasensitive nano UHPLC–ESI–MS/MS method is developed to simultaneously monitor three low-concentration neuromedin-like peptides in microdialysates. Results: Peptide preconcentration and sample desalting performed online on a trap column. A shallow gradient slope at 300 nl/min the analytical column maintained 35°C, followed by two saw-tooth wash cycles, results highest sensitivity lowest carryover. The validated allows accurate precise quantification of 0.5 pM neurotensin...
The pandemic readiness toolbox needs to be extended, targeting different biomolecules, using orthogonal experimental set-ups. Here, we build on our Cov-MS effort LC-MS, adding SISCAPA technology enrich proteotypic peptides of the SARS-CoV-2 nucleocapsid (N) protein from trypsin-digested patient samples. Cov2MS assay is compatible with most matrices including nasopharyngeal swabs, saliva, and plasma has increased sensitivity into attomole range, a 1000-fold improvement compared direct...
This paper describes the investigation of potential a quadrupole orthogonal acceleration time-of-flight mass spectrometer (Q-TOF) equipped with an atmospheric pressure ionisation interface for quantitative measurements small molecules separated by reversed phase liquid chromatography. To this end, detection limits and linear dynamic range in particular were studied LC/MS/MS experiment using 3,4-methylenedioxymethamphetamine standards 3,4-methylenedioxyethylamphetamine internal...
Abstract Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, virus that causes COVID-19, spread so rapidly across globe. The policy response to pandemics will always have include accurate monitoring of spread, this provides one few alternatives total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Although efficient, automatable acceptably cheap,...
Abstract INTRODUCTION The pandemic readiness toolbox needs to be extended, providing diagnostic tools that target different biomolecules, using orthogonal experimental setups and fit-for-purpose specification of detection. Here we build on a previous Cov-MS effort used liquid chromatography-mass spectrometry (LC-MS) describe method allows accurate, high throughput measurement SARS-CoV-2 nucleocapsid (N) protein. MATERIALS METHODS We Stable Isotope Standards Capture by Anti-Peptide Antibodies...