A5005072649- Hormonal and reproductive studies
- Pharmacological Effects and Assays
- Forensic Toxicology and Drug Analysis
- Muscle metabolism and nutrition
- Mass Spectrometry Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- High Altitude and Hypoxia
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- Mycobacterium research and diagnosis
- Analytical Chemistry and Chromatography
- Botulinum Toxin and Related Neurological Disorders
- Diphtheria, Corynebacterium, and Tetanus
- Pesticide Residue Analysis and Safety
- Pharmacogenetics and Drug Metabolism
- Doping in Sports
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Exercise and Physiological Responses
Ghent University
2017-2025
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, virus that causes COVID-19, spread so rapidly across globe. The policy response to pandemics will always have include accurate monitoring of spread, this provides one few alternatives total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by reverse transcription polymerase chain reaction (RT-PCR). Although efficient, automatable, acceptably cheap, reliance on...
Plasticity of skeletal muscle is induced by transcriptional and translational events in response to exercise, leading multiple health performance benefits. The microenvironment harbors myofibers mononuclear cells, but the rich cell diversity has been largely ignored relation exercise adaptations. Using our workflow transcriptome profiling individual myofibers, we observed that their exercise-induced was surprisingly modest compared with bulk tissue response. Through integration single-cell...
Hypoxia-inducible factor (HIF) stabilizers are listed in the World Anti-Doping Agency's prohibited list as they can increase aerobic exercise capacity. The rapid pace of emergence highly structurally diverse HIF could pose a risk to conventional structure-based methods doping control detect new investigational drugs. Therefore, we developed strategy that is capable detecting presence any stabilizer, irrespective its structure, by biological activity. Previously cell-based HIF1/2 assays were...
As hypoxia-inducible factor stabilizers (HIFs) can artificially enhance an athlete's erythropoiesis, the World Anti-Doping Agency prohibits their use at all times. Every urine sample for doping control analysis has to be evaluated presence of HIFs and therefore sensitive methods that allow high throughput are needed. Samples suspicious need confirmed following identification criteria established by Agency. Previous work shown advantages using turbulent flow online solid-phase extraction...
Abstract In doping control, to confirm the exogenous origin of exogenously administered anabolic androgenic steroids (AAS), a gas chromatography combustion isotope ratio mass spectrometry (GC‐C‐IRMS) analysis is performed. Recently published work suggests that epiandrosterone sulfate (EpiAS) promising IRMS target compound for detection AAS, capable prolonging window. However, EpiAS only excreted in urine its sulfoconjugated form, while all other compounds are glucuronidated, meaning cannot...
The most frequently detected substances prohibited by the World Anti-Doping Agency (WADA) belong to anabolic steroids class. challenging compounds among this class are endogenous steroids, which quantitative measurement of testosterone (T) and its metabolites with a so-called "steroid profiling" method. current steroid profile is based on concentrations ratios sum free glucuronidated steroids. Recently, our group developed profiling method for detection three 14 intact conjugates, including...
Abstract Stimulants are often used to treat attention deficit disorders and nasal congestion. As they can be misused overdosed, the detection of stimulants is relevant in toxicological field as well doping control field. The effects indeed beneficial for athletes. Therefore, their in-competition use prohibited by World Anti-Doping Agency (WADA). represent one most detected categories substances, automation methods detect confirm presence desirable. Previous work has shown advantages using...
Abstract Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, virus that causes COVID-19, spread so rapidly across globe. The policy response to pandemics will always have include accurate monitoring of spread, this provides one few alternatives total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Although efficient, automatable acceptably cheap,...