A5064191155- Biochemical effects in animals
- Free Radicals and Antioxidants
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Sports Performance and Training
- Adipose Tissue and Metabolism
- Stress Responses and Cortisol
- Healthcare and Venom Research
- Exercise and Physiological Responses
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Sports injuries and prevention
- Diet, Metabolism, and Disease
- Pharmacological Effects of Natural Compounds
- Nerve injury and regeneration
- GDF15 and Related Biomarkers
- Genetics and Physical Performance
- Cardiovascular Effects of Exercise
- Eating Disorders and Behaviors
- Diet and metabolism studies
- Muscle activation and electromyography studies
- High Altitude and Hypoxia
- Cardiomyopathy and Myosin Studies
- Genetic Neurodegenerative Diseases
- Pharmacological Effects and Assays
- Body Composition Measurement Techniques
University of Copenhagen
2021-2025
Ghent University
2019-2025
Ghent University Hospital
2024
Abstract Exercise promotes brain plasticity partly by stimulating increases in mature brain-derived neurotrophic factor (mBDNF), but the role of pro-BDNF isoform regulation BDNF metabolism humans is unknown. We quantified expression and mBDNF human skeletal muscle plasma at rest, after acute exercise (+/− lactate infusion), fasting. Pro-BDNF were analyzed with immunoblotting, enzyme-linked immunosorbent assay, immunohistochemistry, quantitative polymerase chain reaction. was consistently...
Skeletal muscle is a heterogenous tissue comprised primarily of myofibers, commonly classified into three fiber types in humans: one "slow" (type 1) and two "fast" 2A type 2X). However, heterogeneity between within traditional remains underexplored. We applied transcriptomic proteomic workflows to 1050 1038 single myofibers from human vastus lateralis, respectively. Proteomics was conducted males, while transcriptomics included ten males females. identify metabolic, ribosomal, cell junction...
Considerable inter-individual heterogeneity exists in the muscular adaptations to resistance training. It has been proposed that fast-twitch fibres are more sensitive hypertrophic stimuli and thus variation muscle fibre type composition is a contributing factor magnitude of training response. This study investigated if variability determined by typology most appropriate weekly frequency depends on typology. In strength-training novices, 11 slow (ST) 10 fast (FT) individuals were selected...
The heterogeneous fiber type composition of skeletal muscle makes it challenging to decipher the molecular signaling events driving health- and performance benefits exercise. We developed an optimized workflow for transcriptional profiling individual human fibers before, immediately after, after three hours recovery from high-intensity interval cycling From a point-of-view, we observe that there is no dichotomy in activation, could refer being recruited or non-recruited. Rather, activation...
Histidine-containing dipeptides (HCDs) are pleiotropic homeostatic molecules with potent antioxidative and carbonyl quenching properties linked to various inflammatory, metabolic, neurological diseases, as well exercise performance. However, the distribution metabolism of HCDs across tissues species still unclear.Using a sensitive UHPLC-MS/MS approach an optimized quantification method, we performed systematic extensive profiling in mouse, rat, human body (in n = 26, 25, 19 tissues,...
Balenine possesses some of carnosine's and anserine's functions, yet it appears more resistant to the hydrolysing CN1 enzyme. The aim this study was elucidate stability balenine in systemic circulation its bioavailability humans following acute supplementation. Two experiments were conducted which (in vitro) carnosine, anserine added plasma compare degradation profiles vivo) three increasing doses (1-4-10 mg/kg) acutely administered 6 human volunteers. Half-life (34.9 ± 14.6 min)...
Plasticity of skeletal muscle is induced by transcriptional and translational events in response to exercise, leading multiple health performance benefits. The microenvironment harbors myofibers mononuclear cells, but the rich cell diversity has been largely ignored relation exercise adaptations. Using our workflow transcriptome profiling individual myofibers, we observed that their exercise-induced was surprisingly modest compared with bulk tissue response. Through integration single-cell...
ABSTRACT Purpose This study aimed to investigate the effects of different work and recovery characteristics on W ′ reconstitution test predictive capabilities BAL model. Methods Eleven male participants (22 ± 3 yr, 55 4 mL·kg −1 ⋅min ) completed three five constant rate tests determine CP ′. Subsequently, subjects performed 12 experimental trials, each comprising two exhaustive bouts (i.e., WB1 WB2), interspersed by an active interval. In trial, bout (P4 or P8, i.e., predicted result in...
Histamine H 1 and 2 receptors regulate multiple health-promoting adaptations to exercise training.
Human skeletal muscle has the profound ability to hypertrophy in response resistance training (RT). However, this a high energy and protein cost is presumably mainly restricted recruited muscles. It remains largely unknown what happens with nonrecruited muscles during RT. This study investigated volume changes of 17 13 10-wk single-joint RT program targeting upper arm leg musculature.
Background Recent studies suggest that acute-combined carnosine and anserine supplementation has the potential to improve performance of certain cycling protocols. Yet, data on optimal dose, timing ingestion, effective exercise range, mode action are lacking. Three were conducted establish dosing guidelines concerning intake unravel mechanism underlying ergogenic effects.Methods First, a dose response study A was in which 11 men randomly received placebo, 10, 20, or 30 mg.kg−1 both anserine....
Manipulation of circulating histidine-containing dipeptides (HCD) has been shown to affect the development diabetes and early-stage diabetic nephropathy (DN). The aim present study was investigate whether such interventions, which potentially alter levels HCD, also advanced-stage DN. Two aerobic exercise training overexpression human carnosinase-1 (hCN1) enzyme, were tested. BTBR ob/ob mice either subjected (20 wk) or genetically manipulated overexpress hCN1, different diabetes- DN-related...
Abstract Histidine-containing dipeptides (HCDs) are pleiotropic homeostatic molecules linked to inflammatory, metabolic and neurological diseases, as well exercise performance. Using a sensitive UHPLC-MS/MS approach an optimized quantification method, we performed systematic extensive profiling of HCDs in the mouse, rat, human body (in n=26, n=25, n=19 tissues, respectively). Our data show that tissue HCD levels uniquely regulated by carnosine synthase (CARNS1), enzyme was preferentially...
Abstract Multiple intramuscular variables have been proposed to explain the high variability in resistance training induced muscle hypertrophy across humans. This study investigated if muscular androgen receptor (AR), estrogen α (ERα) and β (ERβ) content fiber capillarization are associated with whole‐muscle after chronic training. Male ( n = 11) female 10) novices (22.1 ± 2.2 years) trained their knee extensors 3×/week for 10 weeks. Vastus lateralis biopsies were taken at baseline post...
Human skeletal muscle fiber type composition varies greatly along the muscle, so one biopsy may not accurately represent whole muscle. Recommendations on number of biopsies and counts using immunohistochemistry whether these findings can be extrapolated to other muscles are lacking. We assessed in vastus lateralis gastrocnemius medialis 40 individuals. Per we took four samples from incision, collecting two each a proximally distally directed needle. Based another dataset involving 10 per...
Abstract Skeletal muscle is an inherently heterogenous tissue comprised primarily of myofibers, which are historically classified into three distinct fiber types in humans: one “slow” (type 1) and two “fast” 2A type 2X), delineated by the expression myosin heavy chain isoforms (MYHs). However, heterogeneity between within traditional remains underexplored. Indeed, whether MYHs main classifiers skeletal fibers has not been examined unbiased manner. Through development application novel...
Introduction: Mitochondria-derived peptides are newly discovered small bioactive thought to communicate metabolic status other cells. Two such peptides, MOTS-c and Humanin, have been shown particularly strong local systemic regulatory effects on metabolism oxidative stress in tissues, but their effect specifically vascular endothelial cells remains unclear. Furthermore, it has proposed that skeletal muscle is a source of humanin during exercise this not directly shown. Methods: In seven...
Personalised dosing of performance-enhancing food supplements is a hot topic. β-alanine currently dosed using fixed dose; however, evidence suggests that this might favour light compared to heavy subjects. A weight-relative dose seems reverse problem. In the present study, novel strategy was tested. fragmented dose, composed fragment 800 mg and 10 mg/kg body weight, 1600 20 weight in cohort subjects with ranging 48–139 kg (79.9 ± 24.4 kg). The results show that, following influence on...
Abstract Multiple sclerosis (MS) pathology features autoimmune-driven neuroinflammation, demyelination, and failed remyelination. Carnosine is a histidine-containing dipeptide (HCD) with pluripotent homeostatic properties that able to improve outcomes in an animal MS model (EAE) when supplied exogenously. To uncover if endogenous carnosine involved in, protects against, MS-related demyelination or remyelination failure, we here studied the HCD-synthesizing enzyme synthase (CARNS1) human...
<b>Background:</b> Carnosine is the archetype of a family histidine-containing dipeptides (HCDs) that play pivotal role in skeletal muscle proton buffering and antioxidant defense. Oral long-term supplementation carnosine precursor beta-alanine (BA) increases persons with COPD, but its effect on other HCDs unclear. <b>Method:</b> We performed highly sensitive targeted liquid-chromatography tandem mass spectrometry m. vastus lateralis plasma samples previously collected for randomized...