A5080012716- Cannabis and Cannabinoid Research
- Forensic Toxicology and Drug Analysis
- Neurotransmitter Receptor Influence on Behavior
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Metabolism, Diabetes, and Cancer
- Muscle metabolism and nutrition
- Hormonal and reproductive studies
- Cancer-related Molecular Pathways
- Eicosanoids and Hypertension Pharmacology
- Psychedelics and Drug Studies
- Effects and risks of endocrine disrupting chemicals
- Mass Spectrometry Techniques and Applications
- Adipose Tissue and Metabolism
- Neuroscience and Neuropharmacology Research
- High Altitude and Hypoxia
Ghent University
2020-2024
Conventionally, hypoxia-inducible factor (HIF) activation by prolyl hydroxylase domain enzyme (PHD) inhibition is monitored gene reporter assays. The principle relies on the monitoring of an upstream event (HIF stabilization) downstream transcriptional activity. Here, we developed a novel approach to directly sense HIF heterodimerization HIFα/HIFβ subunits, constituting functional transcription factor. Two live cell-based biosensor assay setups were designed, utilizing complementation...
Synthetic cannabinoid receptor agonists (SCRAs) are one of the largest groups new psychoactive substances monitored in Europe. SCRAs known to typically exert higher activity than tetrahydrocannabinol from cannabis, thereby entailing a greater health risk. Both Cumyl-PEGACLONE and 5F-Cumyl-PEGACLONE were not controlled by national legislation upon their first detection Germany 2016 2017, respectively, have been linked several fatalities. In this study, CB1 these compounds, together with two...
Synthetic cannabinoid receptor agonists (SCRAs) are amongst the largest groups of new psychoactive substances (NPS). Their often high activity at CB1 frequently results in intoxication, imposing serious health risks. Hence, continuous monitoring these compounds is important, but challenged by rapid emergence novel analogues that missed traditional targeted detection strategies. We addressed this need performing an activity-based, universal screening on a large set (n = 968) serum samples...
Hypoxia-inducible factor (HIF) stabilizers are listed in the World Anti-Doping Agency's prohibited list as they can increase aerobic exercise capacity. The rapid pace of emergence highly structurally diverse HIF could pose a risk to conventional structure-based methods doping control detect new investigational drugs. Therefore, we developed strategy that is capable detecting presence any stabilizer, irrespective its structure, by biological activity. Previously cell-based HIF1/2 assays were...
Over 200 synthetic cannabinoid receptor agonists (SCRAs) have been identified as new psychoactive substances. Effective monitoring and characterization of SCRAs are hindered by the rapid pace structural evolution. Ahead possible appearance on illicit drug market, were synthesized to complete a systematic library cumyl-indole- (e.g., CUMYL-CPrMICA, CUMYL-CPMICA) cumyl-indazole-carboxamides CUMYL-CPrMINACA, CUMYL-CPMINACA), encompassing butyl, pentyl, cyclopropylmethyl, cyclobutylmethyl,...