A5081143881- Biosimilars and Bioanalytical Methods
- Erythropoietin and Anemia Treatment
- Hormonal and reproductive studies
- Mass Spectrometry Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Analytical Chemistry and Chromatography
- Protein purification and stability
- Adrenal Hormones and Disorders
- Metabolomics and Mass Spectrometry Studies
- Leprosy Research and Treatment
University of Amsterdam
2021-2024
Amsterdam University Medical Centers
2021-2024
Vrije Universiteit Amsterdam
2022-2024
Dried blood spot (DBS) sampling has many advantages over conventionally used samples, but is thought to suffer from hematocrit related issues. The aim of our research was investigate whether reliable results can be obtained without bothering about effects in DBS analysis analytes that are mainly present the plasma compartment. Venous samples with variation and spotted volume were prepared. Spot diameter 25-OH Vitamin D3 testosterone concentrations measured. Moreover, D3, determined random...
Therapeutic monoclonal antibodies (mAbs) comprise a large structural variability with respect to charge, size and post-translational modifications. These critical quality attributes (CQAs) need be assessed during after the production of mAbs. This normally requires off-line purification sample preparation as well several chromatographic selectivities, which makes whole process time-consuming error-prone. To improve on this, we developed an integrated automated multi-dimensional analytical...
One of the critical steps during LC-MS/MS hormone analyses that affects sensitivity assay is ionization process. Enhancing efficiencies by addition supercharging reagents might be one way to improve and reduce limit quantification (LOQ). Therefore, we investigated whether m-nitrobenzyl alcohol (m-NBA), sulfolane, propylene carbonate, o-nitroanisole (o-NA) increased efficiency improved LOQ insulin, oxytocin, sex steroids, corticosteroids in test solutions. Additionally, influence was tested...
Over the last years, dried blood spot (DBS) sampling has gained significant interest due to development of analytical techniques combined with DBS, simplicity and low cost method. Despite its wide use, DBS can lead inaccurate results impact hematocrit (Hct) on analysis. Some analytes have shown be hardly impacted by Hct values. However, in other cases, a is observed, which requires use alternative approaches circumvent this issue. This review describes possible Hct-related bias context...