A5004557323- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- RNA Interference and Gene Delivery
- Chemokine receptors and signaling
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Glioma Diagnosis and Treatment
- Cholinesterase and Neurodegenerative Diseases
- Pharmacological Receptor Mechanisms and Effects
- Click Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Cancer Cells and Metastasis
- Nitric Oxide and Endothelin Effects
- Neurotransmitter Receptor Influence on Behavior
- Extracellular vesicles in disease
- Ion channel regulation and function
- Peptidase Inhibition and Analysis
- Phagocytosis and Immune Regulation
- Advanced Glycation End Products research
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Biochemical Analysis and Sensing Techniques
- Silk-based biomaterials and applications
University of Pavia
2015-2024
Istituto di Farmacologia Traslazionale
2019
Activation of the cAMP-dependent protein kinase A (PKA) pathway may induce cAMP-response element-binding (CREB) phosphorylation either directly or via cross-talk mechanisms with other signal transduction pathways. In this study, we have investigated in striatal primary cultures mechanism by which activation cAMP/PKA-dependent leads to CREB extracellular signal-regulated (ERK)-dependent pathway. We found that PKA-induced and CREB-dependent transcription are mediated calcium (Ca<sup>2+</sup>)...
In cancer cells integrins modulate important cellular events that regulate the metastasic cascade which involves detachment from tumor mass, dissemination and attachment to oncogenic niche. The α5β1, αvβ3 αvβ5 are widely expressed in different types recognize tripeptide Arg-Gly-Asp (RGD) motif present several extracellular matrix proteins. human glioblastoma, integrin expression correlates with grade, suggesting this may play a crucial role highly infiltrative behavior of high grade gliomas....
The vasoconstrictor peptide endothelin (ET-1) exerts its physiological and pathological effects via activation of ET A B receptor (ET-R) subtypes. In this study, we demonstrate that both ET-R subtypes are highly expressed in rat astrocytes vivo , indicating these cells potential targets the biological ET-1 brain. cultured cortical astrocytes, expressed, selective stimulation -R with induces phosphorylation cAMP response element-binding protein (CREB). signal transduction pathway activated by...
Metastatic spread is mainly sustained by cancer stem cells (CSC), a subpopulation of that displays stemness features. CSC are thought to be derived from undergo epithelial mesenchymal transition (EMT), thus acquiring resistance anoikis and anti-cancer drugs. After detachment the primary tumor mass, reach blood lymphatic flow, disseminate target tissue. This process nature dynamic in vitro models quite far vivo situation. In this study, we have tried reproduce adhesion tissue using 3D cell...
Developing drug delivery systems to target cytotoxic drugs directly into tumor cells is still a compelling need with regard reducing side effects and improving the efficacy of cancer chemotherapy. In this work, silk fibroin nanoparticles (SFNs) have been designed load previously described compound (NDI-1) that disrupts cell cycle by specifically interacting non-canonical secondary structures DNA. SFNs were then functionalized on their surface cyclic pentapeptides incorporating Arg-Gly-Asp...
The neurotransmitter dopamine (DA) at a 10 μM concentration elicited stimulation of intracellular cyclic AMP (cAMP) accumulation in cultured astrocytes derived from embryonic rat striatum. This was partially blocked by the β-adrenergic receptors antagonist propranolol, mimicked D1 agonist SKF 38393 and mixed D1/D2 apomorphine. A regional heterogeneity magnitude dopamine-induced cAMP observed obtained different brain areas. maximum effect striatal astrocytes, lower cortical no increase...
Abstract The cyclo [DKP‐ iso DGR] peptidomimetics 2 – 5 , containing bifunctional diketopiperazine (DKP) scaffolds that differ in the configuration of two DKP stereocenters and substitution at nitrogen atoms, were prepared examined vitro competitive binding assays with purified α v β 3 integrin receptors. IC 50 values ranged from low nanomolar (ligand ) to submicromolar integrin. biological activities ligands [DKP3‐RGD] 1 [DKP3‐ bearing same scaffold showing similar V values, compared terms...
Arg-Gly-Asp (RGD)-based cyclopentapeptides (cRGDs) have a high affinity towards integrin αvβ3 and αvβ5, which are overexpressed by many tumor cells. Here, curcumin-loaded silk fibroin nanoparticles (SFNs) been functionalized on the surface with cRGD to provide active targeting cells; “click reaction” between RGD-based cyclopentapeptide carrying an azide group triple-bond-functionalized has exploited. Both naked SFNs were less than 200 nm in diameter showed round-shaped morphology but, after...
Abstract: D 1 ‐and 2 ‐dopamine receptors exert important physiological actions on striatal neurons, but the intracellular second messenger pathways activated by these are still incompletely understood. Using primary cultures of rat cells, we have examined effects activating or arachidonic acid (AA) release and cyclic AMP accumulation. In neurons labeled incubation with [ 3 H]AA, ‐receptor stimulation enhanced H]AA produced application Ca 2+ ionophore A23187 purinergic agonist ATP. By...
Extracellular vesicles (EVs) are considered as promising nanoparticle theranostic tools in many pathological contexts. The increasing clinical employment of therapeutic nanoparticles is contributing to the development a new research area related design artificial EVs. To this aim, different approaches have been described develop mimetic biologically functional nanovescicles. In paper, we suggest simplified procedure generate plasma membrane-derived nanovesicles with possibility efficiently...
We analyzed the expression and relative distribution of mRNA for regulatory subunits (RIalpha, RIIalpha, RIIbeta) 150-kDa RIIbeta-anchor proteins cyclic AMP (cAMP)-dependent protein kinase (PKA) into discrete brain regions. The subcellular both holoenzyme free catalytic subunit was evaluated in same CNS areas. In neocortex corpus striatum high levels RIIbeta paralleled presence specific RII-anchoring proteins, membrane-bound PKA holoenzyme, low cytosolic activity (C-PKA). Conversely, areas...
The malignancy of glioblastoma (GB) is primarily due to the ability glioma cancer stem cells (GSC) disseminate into surrounding brain tissues, despite surgery and chemotherapy, form new tumoral masses. Members RGD-binding integrin family, which recognize arginine-glycine-aspartic acid (RGD) sequence present in components extracellular matrix, serve a crucial function dissemination GCS, are overexpressed GB. Small-molecule antagonists (SMIAs) designed RGD-integrins may therefore be an...
Photodynamic therapy (PDT) has recently attracted interest as an innovative and adjuvant treatment for different cancers including malignant gliomas. Among these, Glioblastoma (GBM) is the most prevalent neoplasm in central nervous system. Despite conventional therapeutic approaches that include surgical removal, radiation, chemotherapy, GBM characterized by extremely poor prognosis a high rate of recurrence. PDT physical process induces tumor cell death through genesis accumulation reactive...
cAMP signals are received and transmitted by multiple isoforms of cAMP-dependent protein kinases, typically determined their specific regulatory subunits. In the brain major isoform RIIbeta RII-anchor protein, AKAP150 (rat) or 75 (bovine), differentially expressed. Cortical neurons express AKAP75; conversely, granule cerebellar cells predominantly RIalpha RIIalpha. accumulate PKA catalytic subunit phosphorylated responsive element binding very efficiently into nuclei upon induction, whereas...