A5013286986- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Nanoplatforms for cancer theranostics
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- Nanoparticle-Based Drug Delivery
- Metal complexes synthesis and properties
- Ferrocene Chemistry and Applications
- Biochemical Analysis and Sensing Techniques
- Plant Gene Expression Analysis
- Medicinal Plants and Bioactive Compounds
- Trypanosoma species research and implications
- Congenital gastrointestinal and neural anomalies
- Hearing, Cochlea, Tinnitus, Genetics
- Berberine and alkaloids research
- Insect symbiosis and bacterial influences
- Heat shock proteins research
University of Pavia
2019-2025
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2023
IP
Aging and its related disorders are important issues nowadays the first cause of this physio-pathological condition is overproduction ROS. Ascorbic acid an antioxidant mediator anti-aging proprieties well known. Our previous data demonstrated that Voghera sweet pepper (VP), a distinctive type cultivated in Italy, particularly rich ascorbic acid. Based on these data, effect mediated by extracts edible part VP was evaluated vitro model both young old Normal Human Diploid Fibroblasts (NHDF)....
ABSTRACT Due to the lack of effective therapeutic approach, glioblastoma (GBM) remains one most malignant brain tumour. By in vitro investigations on primary GBM stem cells, we highlighted underlying mechanisms drug resistance alkylating agents, DNA damage responses. Here, flow cytometric analysis and viability repopulation assays were used assess long‐term cytotoxic effect induced by administration a fourth‐generation platinum prodrug, ( OC...
The proof-of-principle of the therapeutic potential heat shock protein 47 (HSP47) for diseases characterized by defects in collagen I synthesis is here proved osteogenesis imperfecta (OI), a prototype disorders. Most OI mutations delay chains folding, increasing their exposure to post translational modifications that affect secretion and impact extracellular matrix fibrils assembly. As model, we used primary fibroblasts from individuals with defect prolyl-3-hydroxylation complex, since are...
Alterations of endoplasmic reticulum (ER)-mitochondrial interaction have been associated with different pathological conditions, including neurodegenerative diseases, characterized by dysregulation protein homeostasis. However, little is known about how enhanced ER-mitochondrial tethering affects cellular proteostatic machinery. Here, we transiently overexpressed synthetic linkers (EMLs), stabilizing the distance at ≤5 nm (denominated as 5 nm-EML) and ∼10 (10 nm-EML), in HeLa cells. No...
Abstract Tsetse flies are insects of significant public health and zoonotic importance as they the main vectors African trypanosomes. To date, an effective vaccine is unavailable efforts to limit spread disease primarily rely on controlling tsetse populations. The discovery Spiroplasma (class Mollicutes) in Glossina fuscipes ( Gff ) palpalis subgroup), offers promising insights into its potential use a biological control agent hinder trypanosomes infection flies. Indeed, negative correlation...
The current standard oncotherapy for glioblastoma is limited by several adverse side effects, leading to a short-term patient survival rate paralleled worsening quality of life (QoL). Recently, Complementary and Integrative Medicine’s (CIM) innovative approaches have shown positive impacts in terms better response treatment, effect reduction, QoL improvement. In particular, promising potential cancer therapy has been found compounds coming from phyto- mycotherapy. objective this study was...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a newly discovered second messenger that gates two pore channels 1 (TPC1) and 2 (TPC2) to elicit endo-lysosomal (EL) Ca2+ release. NAADP-induced lysosomal release may be amplified by the endoplasmic reticulum (ER) through Ca2+-induced (CICR) mechanism. intracellular signals were shown modulate growing number of functions in cardiovascular system, but their occurrence role cardiac mesenchymal stromal cells (C-MSCs) still unknown....
Cells are continuously exposed to various sources of insults, among which temperature variations extremely common. Epigenetic mechanisms, critical players in gene expression regulation, undergo alterations due these stressors, potentially leading health issues. Despite the significance DNA methylation and histone modifications their changes following heat cold shock human cells remain poorly understood. In this study, we investigated epigenetic profiles subjected hyperthermia hypothermia,...
Photodynamic therapy (PDT) has recently attracted interest as an innovative and adjuvant treatment for different cancers including malignant gliomas. Among these, Glioblastoma (GBM) is the most prevalent neoplasm in central nervous system. Despite conventional therapeutic approaches that include surgical removal, radiation, chemotherapy, GBM characterized by extremely poor prognosis a high rate of recurrence. PDT physical process induces tumor cell death through genesis accumulation reactive...
Abstract One hundred years ago, Robert Feulgen published a landmark paper in which he described the first method to stain DNA cells and tissues. Although century has passed since discovery by Rossenbeck, chemical reaction still exerts an important influence current histochemical studies. Its contribution diverse fields, spanning from biomedicine plant biology, paved way for most significant studies that constitute our knowledge. The possibility specifically explore cell nuclei while...
Photodynamic therapy (PDT) is a promising anticancer strategy based on the light energy stimulation of photosensitizers (PS) molecules within malignant cell. Among multitude recently challenged PS, Rose bengal (RB) has been already reported as an inducer cytotoxicity in different tumor cells. However, RB displays low penetration capability across cell membranes. We have therefore developed short-term amino acids starvation protocol that significantly increases uptake human astrocytoma cells...
Malignant primary brain tumors remain among the most difficult cancers to treat, in particular, Glioblastoma Multiforme (GBM) is deadliest tumor. The standard therapies currently used are not efficient enough improving patients' survival and quality of life. Cisplatin (CDDP), a platinum-based drug, has shown efficacy against different solid neoplasms, but it also associated forms off-target toxicity. To overcome limitation use CDDP treatment GBM patients, fourth generation platinum compounds...
Enteric glial cells (EGCs), the major components of enteric nervous system (ENS), are implicated in maintenance gut homeostasis, thereby leading to severe pathological conditions when impaired. However, due technical difficulties associated with EGCs isolation and cell culture that results a lack valuable vitro models, their roles physiological contexts have been poorly investigated so far. To this aim, we developed for first time, human immortalized EGC line (referred as ClK clone) through...
The effects of ferrocene (Fc) and ferrocenium (Fc+) induced in triple negative human breast cancer MCF-7 cells were explored by immunofluorescence, flow cytometry, transmission electron microscopy analysis. different abilities Fc Fc+ to produce reactive oxygen species induce oxidative stress clearly observed activating apoptosis morphological changes after treatment, but also tests performed on the model organism D. discoideum, particularly case Fc+. induction ferroptosis, an iron-dependent...
Cell genome integrity is continuously threatened by various sources, both endogenous and exogenous. Oxidative stress causes a multitude of damages, severely affecting cell viability, fidelity genetic information inheritance, determining profound alterations in gene expression. Epigenetics represents major form expression modulation, influencing DNA accessibility to transcription factors the overall nuclear architecture. When assessing stress-induced epigenome reprogramming, widely diffused...
The evolution of chemical signals is subject to environmental constraints. A multicomponent signal may combine semiochemical molecules with supporting compounds able enhance communication efficacy. Carbonic anhydrases (CAs) are ubiquitous enzymes catalysing the reversible hydration carbon dioxide, a reaction involved in variety physiological processes as it controls environment different tissues or cellular compartments, thus contributing overall system homeostasis. CA-IV isoform has been...
ABSTRACT IP 3 receptor (IP R)-mediated Ca 2+ transfer at the mitochondria-endoplasmic reticulum (ER) contact sites (MERCS) drives mitochondrial uptake and oxidative metabolism is linked to different pathologies, including Parkinson’s disease (PD). The dependence of efficiency on ER-mitochondria distance remains unexplored. Employing molecular rulers that stabilize ER-mitochondrial distances 5 nm resolution, using genetically-encoded indicators targeting ER lumen sub-mitochondrial...
Malignant mesothelioma is a rare tumor associated with asbestos exposure. Mesothelioma carcinogenesis related to enhanced reactive oxygen species (ROS) production and iron overload. Despite the recent advances in biomedical sciences, date only available treatments include surgery small fraction of patients platinum-based chemotherapy combination pemetrexed. In this view, purpose study was evaluate therapeutic potential newly synthetized platinum prodrug Pt(IV)Ac-POA compared cisplatin (CDDP)...
As nanocarriers of a new generation, biomimetic nanovesicles are an emerging class therapeutic tools whose surface is integrated or fabricated with biomaterials capable mimicking the biological features and functions native cells. Thanks to this, nanovesicles, in particular, those made by plasma membrane moieties, possess greatly improved biocompatibility, high target specificity, long retention time, minimal undesired immune responses. For these reasons, multitude progenitor cells including...
Mercury is a highly toxic element that induces severe alterations and broad range of adverse effects on health. Its exposure global concern because it widespread in the environment due to its multiple industrial, domestic, agricultural medical usages. Among various chemical forms, both humans animals are mainly exposed mercury chloride (HgCl2), methylmercury elemental mercury. HgCl2 metabolized primarily liver. We analysed nuclear architecture an increasing dosage mouse hepatocytes cell...