A5015027835- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Environmental Toxicology and Ecotoxicology
- Physiological and biochemical adaptations
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Diabetes Treatment and Management
- Heart Failure Treatment and Management
- RNA and protein synthesis mechanisms
- Nanoparticle-Based Drug Delivery
- Vitamin D Research Studies
- Insect and Pesticide Research
- Retinoids in leukemia and cellular processes
- Neurobiology and Insect Physiology Research
- Genomics, phytochemicals, and oxidative stress
- Natural product bioactivities and synthesis
- Photoacoustic and Ultrasonic Imaging
- Neuroblastoma Research and Treatments
- Nanoparticles: synthesis and applications
- Diet, Metabolism, and Disease
- Calcium signaling and nucleotide metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Plant Gene Expression Analysis
- Occupational and environmental lung diseases
University of Pavia
2008-2024
Associazione Nazionale Medici Cardiologi Ospedalieri
2017
Istituto di Genetica Molecolare
2008
Aging and its related disorders are important issues nowadays the first cause of this physio-pathological condition is overproduction ROS. Ascorbic acid an antioxidant mediator anti-aging proprieties well known. Our previous data demonstrated that Voghera sweet pepper (VP), a distinctive type cultivated in Italy, particularly rich ascorbic acid. Based on these data, effect mediated by extracts edible part VP was evaluated vitro model both young old Normal Human Diploid Fibroblasts (NHDF)....
Although environmental airborne silver nanoparticles (AgNPs) levels in occupational and settings are harmful to humans, the precise toxic effects at portal entry of exposure after translocation distant organs still be deeply clarified. To this aim, present study assessed histopathological ultrastructural alterations (by means H&E TEM, respectively) rat lung liver, 7 28 days a single intratracheal instillation (i.t) low AgNP dose (50 microg/rat), compared those induced by an equivalent ionic...
Nonalcoholic fatty liver disease (NAFLD) is a serious health problem in developed countries. We documented the effects of feeding with NAFLD-inducing, methionine- and choline-deficient (MCD) diet, for 1-4 weeks on rat oxidative stress, respect to control diet. Glycogen, neutral lipids, ROS, peroxidated proteins, SOD2 were investigated using histochemical procedures; ATP, GSH, TBARS concentrations by biochemical dosages, expression was Western Blotting. In 4-week-diet period, glycogen stores...
This study compares the effects of machine perfusion (MP) at different temperatures with simple cold storage. In addition, role Ca2+ levels in MP medium was evaluated. For MP, rat livers were perfused for 6 hours Krebs-Henseleit (KH) solution (with 1.25 or 2.5 mM CaCl2) 4°C, 10°C, 20°C, 25°C, 30°C, 37°C. storage, situ and preserved Celsior 4°C hours. The reperfusion period (2 37°C) performed under same conditions used MP-preserved storage–preserved livers. Hepatic enzyme release, bile...
Machine perfusion at subnormothermic temperature (20°C), MP20, was developed by Vairetti et al. and showed to afford a better preservation of fatty livers respect traditional cold storage (CS) in terms enzyme release into the perfusate bile, glycogen stores, energy charge oxidative stress. Here we investigated whether it also caused decreased cell death apoptosis. Fatty lean Zucker rats were submitted MP20 or CS for 6 h reperfused normothermically 2 h. Apoptotic cells revealed...
Given the scarcity of donors, moderately fatty livers (FLs) are currently being considered as possible grafts for orthotopic liver transplantation (OLT), notwithstanding their poor tolerance to conventional cold preservation. The behaviour parenchymal and sinusoidal cells during is studied worldwide. Much less attention has been paid biliary tree, although this Achille's heel even normal transplantation. To evaluate response compartment FLs various phases OLT reliable markers necessary....
Photodynamic therapy (PDT) has recently attracted interest as an innovative and adjuvant treatment for different cancers including malignant gliomas. Among these, Glioblastoma (GBM) is the most prevalent neoplasm in central nervous system. Despite conventional therapeutic approaches that include surgical removal, radiation, chemotherapy, GBM characterized by extremely poor prognosis a high rate of recurrence. PDT physical process induces tumor cell death through genesis accumulation reactive...
Photodynamic therapy (PDT) is a promising anticancer strategy based on the light energy stimulation of photosensitizers (PS) molecules within malignant cell. Among multitude recently challenged PS, Rose bengal (RB) has been already reported as an inducer cytotoxicity in different tumor cells. However, RB displays low penetration capability across cell membranes. We have therefore developed short-term amino acids starvation protocol that significantly increases uptake human astrocytoma cells...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on the Mechanism of Action Gentamicin. Effect Protein Synthesis in Cell-Free Extracts Escherichia coli*G. Milanesi and O. CiferriCite this: Biochemistry 1966, 5, 12, 3926–3935Publication Date (Print):December 1, 1966Publication History Published online1 May 2002Published inissue 1 December 1966https://pubs.acs.org/doi/10.1021/bi00876a026https://doi.org/10.1021/bi00876a026research-articleACS PublicationsRequest reuse permissionsArticle...
Oxidative stress in fatty livers is mainly generated by impaired mitochondrial β -oxidation, inducing tissue damages and disease progression. Under suitable excitation, light liver endogenous fluorophores can give rise to autofluorescence (AF) emission, the properties of which depend on organ morphofunctional state. In this work, we characterized AF a rat model lipid accumulation oxidative stress, induced 1–9-week hypercaloric methionine-choline deficient (MCD) diet administration. The...
Abstract In the Medicago genus, saponins are complex mixtures of triterpene pentacyclic glycosides extensively studied for their different and economically relevant biological pharmaceutical properties. This research is aimed at determining first time tissue cellular localization in vegetative organs truncatula , a model plant species legumes, by histochemistry transmission electron microscopy. The results showed that present mainly palisade mesophyll layer leaves, whereas stems they mostly...
The aim of this phase IV study was to assess the tolerability and efficacy acipimox, a lipid-lowering drug, in 3009 type II diabetic out-patients with types hyperlipoproteinaemia. carried out by 150 Italian diabetes centres. Acipimox given at dosage one capsule (250 mg), two or three times daily for least 2 months. produced mean fall 43% serum triglycerides 18% total cholesterol levels compared baseline. effect present throughout treatment, but most pronounced end treatment. An increase...
Graft steatosis is a risk factor for poor initial function after liver transplantation. Biliary complications are frequent even normal A subnormothermic machine perfusion (MP20) preservation procedure was developed by our group with high potential reducing injury to hepatocytes and sinusoidal cells of lean fatty livers respect conventional cold storage (CS). We report the response biliary tree CS or MP20, in obese Zucker rat liver. Dipeptidylpeptidase-IV (DPP-IV), crucial inactivation...
The identification of new biomarkers allowing an early and more accurate characterization patients with ST-segment elevation myocardial infarction (STEMI) is still needed, exosomes represent attractive diagnostic tool in this context. However, the their protein cargo relation to cardiovascular clinical manifestation lacking. To end, 35 STEMI (17 experiencing resuscitated out-of-hospital cardiac arrest (OHCA-STEMI) 18 uncomplicated) 32 chronic coronary syndrome (CCS) were enrolled. Plasma...
Abstract Over the last 50 years, many species of amphibians have declined markedly, and even become extinct throughout world. The cause this is not clear, several factors may be involved, including exposure to contaminants. effects some pollutants (i.e., chromium heptachlor epoxide) on green frog (.Rana kl. esculenta) were assessed experimentally, using morpho‐histological techniques. At lowest doses used (0.5 ppm for Cr, 4 ppb ‐ 40 epoxide), hepatocytes showed no relevant structural or...