A5078462901- Cell death mechanisms and regulation
- Metal complexes synthesis and properties
- RNA Interference and Gene Delivery
- Fungal Biology and Applications
- Photodynamic Therapy Research Studies
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Glioma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Medicinal Plants and Bioactive Compounds
- Immunotherapy and Immune Responses
- Cancer-related cognitive impairment studies
- DNA Repair Mechanisms
- Nanoplatforms for cancer theranostics
- Nuclear Structure and Function
- Ferrocene Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Histone Deacetylase Inhibitors Research
- Phytochemistry and Bioactivity Studies
- RNA regulation and disease
- Neurogenesis and neuroplasticity mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Phytochemistry and Biological Activities
University of Pavia
2016-2025
Biogem
2017-2021
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2019
University of Salento
2018
Istituto di Genetica Molecolare
2006-2014
Istituto Nazionale di Fisica Nucleare, Sezione di Pavia
2011
National Research Council
2009
Institute of Cell Biology and Neurobiology
2009
Fondazione IRCCS Istituto Nazionale dei Tumori
2001
Policlinico San Matteo Fondazione
1998
An apoptotic program leading to controlled cell dismantling implies perturbations of nuclear dynamics, as well changes affecting the organelle structure and distribution. In human cancer cells driven apoptosis by different stimuli, we have recently investigated morphological properties several organelles, including mitochondria, lysosomes, endoplasmic reticulum Golgi apparatus. this review, will discuss body evidence in literature suggesting that organelles are generally relocated and/or...
Frailty is a geriatric syndrome associated with both locomotor and cognitive decline, implicated in poor quality of life negative health outcomes. One central question surrounding frailty whether phenotypic the impairment during aging. Using spontaneous behavioral tests by studying dynamic change aging, we demonstrated that two form vulnerability, recognition memory develop parallel therefore, integration motoric evaluations are imperative. We developed an integrated index based on...
Frailty is a geriatric syndrome associated with both locomotor and cognitive decline, typically linked to chronic systemic inflammation, i.e., inflammaging. In the current study, we investigated effect of two-month oral supplementation standardized extracts H. erinaceus, containing known amount Erinacine A, Hericenone C, D, L-ergothioneine, on frailty cerebellum aged mice. Locomotor performances were monitored comparing healthy aging frail Cerebellar volume cytoarchitecture, together...
Brain aging is a crucial risk factor for several neurodegenerative disorders and dementia. The most affected cognitive function memory, worsening early during aging. Inflammation oxidative stress are known to have role in pathogenesis of impairments, link exists between aging/frailty immunosenescence/inflammaging. Based on anti-aging properties, medicinal mushrooms represent source develop medicines functional foods. In particular, Hericium erinaceus (He) displays actions ranging from...
Phenotypic frailty is characterized by a progressive decline in physical functioning. During ageing, morphological and functional alterations involve the brain, chief theories oxidative stress, free radical accumulation, reduced antioxidant defenses as most implicated mechanisms. From boosting immune system to fighting senescence, medicinal mushrooms have been found number of health longevity benefits. Among them, Hericium erinaceus (He) has demonstrated display variety physiological...
The design, synthesis, characterization and biological properties of a Pt(iv) complex containing the very active inhibitor histone deacetylase (2-propynyl)octanoic acid, POA, as an axial ligand are reported here. title complex, namely (OC-6-44)-acetatodiamminedichlorido(2-(2-propynyl)octanoato)platinum(iv), 1, POA in racemic or enantiomeric forms, was one/two orders magnitude more than cisplatin, depending on chemo-sensitivity cancer cell lines. Moreover, 1 exhibited similar even better...
Aging and its related disorders are important issues nowadays the first cause of this physio-pathological condition is overproduction ROS. Ascorbic acid an antioxidant mediator anti-aging proprieties well known. Our previous data demonstrated that Voghera sweet pepper (VP), a distinctive type cultivated in Italy, particularly rich ascorbic acid. Based on these data, effect mediated by extracts edible part VP was evaluated vitro model both young old Normal Human Diploid Fibroblasts (NHDF)....
ABSTRACT Due to the lack of effective therapeutic approach, glioblastoma (GBM) remains one most malignant brain tumour. By in vitro investigations on primary GBM stem cells, we highlighted underlying mechanisms drug resistance alkylating agents, DNA damage responses. Here, flow cytometric analysis and viability repopulation assays were used assess long‐term cytotoxic effect induced by administration a fourth‐generation platinum prodrug, ( OC...
Vascular smooth muscle cells derived from the rat aorta were cultured on unmodified or F(+) ion-implanted polystyrene (5 x 10(12) 5 10(14) ions/cm(2), energy 150 keV). In 1-day-old cultures, adhered to modified in higher numbers and over larger contact areas. Increased resistance of trypsin-mediated detachment growth support indicated an improved adhesion polymer at later culture intervals. The ion-modified polymers also had a total protein content. By use immunocytochemistry, several...
Although environmental airborne silver nanoparticles (AgNPs) levels in occupational and settings are harmful to humans, the precise toxic effects at portal entry of exposure after translocation distant organs still be deeply clarified. To this aim, present study assessed histopathological ultrastructural alterations (by means H&E TEM, respectively) rat lung liver, 7 28 days a single intratracheal instillation (i.t) low AgNP dose (50 microg/rat), compared those induced by an equivalent ionic...
Breast cancer (BC) is the second most common cause of brain metastasis onset in patients, with cerebellum accounting for 33% cases. In current study, using a 4T1 triple-negative mouse BC model, we revealed that an orally administered medicinal mushrooms (MM) blend, rich β-glucans, played direct and specific anti-cancer action on cerebellar metastases, also bettering locomotor performances. The neuroprotective effect MM blend plays through (i) inhibition metastatization pattern typical TNBC...
During spontaneous apoptosis of thymocytes there is extrusion ribonucleoproteins (RNPs) from the cell. The aim this investigation was to elucidate whether RNP aggregates in apoptotic cells and bodies still contain RNA an appreciable amount. We demonstrated by specific cytochemical techniques that nuclear RNPs extruded cytoplasm spontaneously a sufficient amount be detected cytochemically. These heterogeneous ectopic RNP-derived structures (HERDS) are formed perichromatin fibrils,...
Cisplatin (cisPt) is used as a chemotherapeutic agent for the treatment of variety human tumours; more recently, it has been demonstrated that tumour cell exposure to cisPt ultimately results in apoptosis, but mechanism by which nuclear cisPt/DNA generates cytoplasmic cascade events involved not clarified. We have investigated effects on proliferation neuronal line B50, with particular attention being given understand whether mitochondria are target and their involvement apoptotic...
Although medicinal mushroom extracts have been proposed as promising anti-cancer agents, their precise impacts on metastatic breast cancer are still to be clarified. For this purpose, the present study exploited effect of a novel blend, namely Micotherapy U-care, in 4T1 triple-negative mouse model. Mice were orally administered with consisting mixture Agaricus blazei, Ophiocordyceps sinensis, Ganoderma lucidum, Grifola frondosa, and Lentinula edodes. The syngeneic tumor-bearing mice...
SUMMARY Microfluorimetric and flow cytometric analyses were used in nuclei of cortical cells roots the endomycorrhizal system Allium porrum L. + a Glomus sp., strain E 3 (from Rothamsted Station), an attempt to explain nuclear hypertrophy. No variation DNA content comparison with controls was observed mycorrhizal roots. An increase fluorescence (about 25 %) root stained undersaturating concentration DAPI. This can be explained by greater accessibility fluorochromes owing lower degree...
Comparative studies on the effects of platinum complexes in use or clinical trials are carried out order to discover differences neurotoxic potential and reversibility neurotoxicity. In this paper, we summarized current literature neurotoxicity chemoresistance cisplatin (cisPt) discussed our recent efforts interference cisPt a new compound [Pt(O,O ′ -acac)(γ-acac)(DMS)] (PtAcacDMS), with high specific reactivity sulphur ligands instead nucleobases as cisPt, some crucial events rat postnatal...