A5007949616- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Mitochondrial Function and Pathology
- Vestibular and auditory disorders
- Neurobiology and Insect Physiology Research
- Metabolism and Genetic Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- DNA and Nucleic Acid Chemistry
- Cell death mechanisms and regulation
- Adipose Tissue and Metabolism
- Molecular Sensors and Ion Detection
- Anesthesia and Neurotoxicity Research
- Amino Acid Enzymes and Metabolism
- Lipid Membrane Structure and Behavior
- Neuropeptides and Animal Physiology
- Biochemical effects in animals
- DNA Repair Mechanisms
- Physiological and biochemical adaptations
- Bat Biology and Ecology Studies
- Cancer-related cognitive impairment studies
- Glioma Diagnosis and Treatment
- Metal complexes synthesis and properties
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Insect behavior and control techniques
University of Pavia
2010-2019
Istituto di Genetica Molecolare
2003-2012
Institute of Cell Biology and Neurobiology
2009
National Research Council
1974-1990
University of Turin
1990
Czech Academy of Sciences
1986
Czech Academy of Sciences, Institute of Physiology
1985-1986
An apoptotic program leading to controlled cell dismantling implies perturbations of nuclear dynamics, as well changes affecting the organelle structure and distribution. In human cancer cells driven apoptosis by different stimuli, we have recently investigated morphological properties several organelles, including mitochondria, lysosomes, endoplasmic reticulum Golgi apparatus. this review, will discuss body evidence in literature suggesting that organelles are generally relocated and/or...
Cisplatin (cisPt) is used as a chemotherapeutic agent for the treatment of variety human tumours; more recently, it has been demonstrated that tumour cell exposure to cisPt ultimately results in apoptosis, but mechanism by which nuclear cisPt/DNA generates cytoplasmic cascade events involved not clarified. We have investigated effects on proliferation neuronal line B50, with particular attention being given understand whether mitochondria are target and their involvement apoptotic...
Mild pulmonary interstitial edema was shown to cause fragmentation of matrix proteoglycans. We therefore studied compartmental fluid accumulation by light and electron microscopy on lungs anesthetized rabbits fixed in situ vascular perfusion after 0.5 ml · kg −1 min iv saline infusion for 180 causing ∼6% increase lung weight. Morphometry showed that a relevant portion (44%) extravascular is detected early the alveolar septa, 85% this accumulating thick air-blood barrier. The arithmetic mean...
Comparative studies on the effects of platinum complexes in use or clinical trials are carried out order to discover differences neurotoxic potential and reversibility neurotoxicity. In this paper, we summarized current literature neurotoxicity chemoresistance cisplatin (cisPt) discussed our recent efforts interference cisPt a new compound [Pt(O,O ′ -acac)(γ-acac)(DMS)] (PtAcacDMS), with high specific reactivity sulphur ligands instead nucleobases as cisPt, some crucial events rat postnatal...
We evaluated the relationship among proliferation, death and migration of granule cells in lobules VI–VIII vermis, comparison with lobule III, during cerebellar development. To this aim, a single injection cisplatin, i.e., cytostatic agent that is known to induce proliferating cells, was given 10-day-old rats. Histochemical markers (PCNA immunoreaction) apoptotic (TUNEL staining) were used; variations external granular layer (EGL) thickness parallel. After PCNA TUNEL reactions, evident...
Abstract Replicative synthesis of DNA in the brain adult frog was studied by light microscope autoradiography. Animals collected during active period (May–June) and hibernation (January) were used. In frogs, 3H‐thymidine labelling occurred mainly ependymal cells which line ventricles. The mean index (LI%) higher ependyma lateral fourth ventricles than diencephalon tectal parts mesencephalon. recessus infundibularis preopticus number labelled (LCs) several times greater third ventricle. LCs...
'Binomial' cell proliferation and death have been studied in only a few non-mammalian vertebrates, such as fish. We thought it of interest to map proliferation/apoptosis the brain frog (Rana esculenta L.) this animal species undergoes, during annual cycle, physiological events that could be associated with central nervous system damage. Therefore, we compared active period deep underground hibernation frog. Using western blot analysis for proliferating nuclear antigen (PCNA), revealed...
Cisplatin (cisPt) is a chemotherapeutic drug used for several human malignancies. CisPt cytotoxicity primarily mediated by its ability to cause DNA damage and subsequent apoptotic cell death. the primary target of cisPt; however, recent data have shown that cisPt may important direct interactions with mitochondria, which can induce apoptosis account significant part clinical activity associated this drug. We previously demonstrated in rat neuronal line B50, at 20 h-treatment activates...
The role of some neuromodulators and neurotransmitters in the functioning molluskan cerebral neurons their metabolic changes during hibernation has been considered. ganglion mollusks is a center for integration different inputs from sensory areas head generation motor command impulses. During hibernation, animals are deprived many external stimuli do not have locomotion feeding. Immunocytochemistry bioactive peptides (BAPs), such as SP (Substance P), CCK8 (Cholecystokinin 8/Gastrin), CGRP...
Cisplatin (cisPt) is a chemotherapy agent used as treatment for several types of cancer. The main cytotoxic effect cisplatin generally accepted to be DNA damage. Recently, the mechanism by which cisPt generates cascade events involved in apoptotic process has been demonstrated. In particular it shown that some organelles are target and cell death. This paper aims describe morphological functional changes Golgi apparatus lysosomes during apoptosis induced neuronal rat cells (B50) cisplatin....
Abstract A natural defect in rat cerebellum postnatal development has been found the fissura prima, consisting various complex configurations of cerebellar layers. We investigated genesis fissure malformations through immunoreactions for PCNA, GFAP, GABAA α6, and calbindin to label proliferating cells external granular layer (egl), radial glial fibers, mature granule cells, Purkinje respectively. Results on critical stages provided interesting evidences how malformation develops fissures...