A5079166399- Respiratory Support and Mechanisms
- Inhalation and Respiratory Drug Delivery
- Chronic Obstructive Pulmonary Disease (COPD) Research
- High Altitude and Hypoxia
- Neonatal Respiratory Health Research
- Neuroscience of respiration and sleep
- Pleural and Pulmonary Diseases
- Pulmonary Hypertension Research and Treatments
- Proteoglycans and glycosaminoglycans research
- Asthma and respiratory diseases
- Cardiovascular and exercise physiology
- Caveolin-1 and cellular processes
- Airway Management and Intubation Techniques
- Heart Rate Variability and Autonomic Control
- Hemoglobin structure and function
- Spaceflight effects on biology
- Hemodynamic Monitoring and Therapy
- Erythrocyte Function and Pathophysiology
- Cancer, Hypoxia, and Metabolism
- Cardiovascular Syncope and Autonomic Disorders
- Atomic and Subatomic Physics Research
- Adipose Tissue and Metabolism
- Epilepsy research and treatment
- Lung Cancer Diagnosis and Treatment
- Occupational and environmental lung diseases
University of Milano-Bicocca
2014-2024
Victoria University
2016
University of Milan
1991-2015
National Bank of Romania
2010
University of Salento
2010
The University of Western Australia
2000-2007
Edith Cowan University
2007
Istituto Nazionale di Fisica Nucleare, Laboratori Acceleratori e Superconduttività Applicata
2007
Institute of Agricultural Biology and Biotechnology
2003
University of Insubria
2001
The present project involved a collective effort agreed by the European Society of Thoracic Surgeons, American Association for Surgery, and General Surgery Club to assemble joint panel experts review available data address ambiguous aspects chest tube definitions nomenclature. task force was composed 11 invited participants, identified their expertise in area management. subject divided different topics, which were turn assigned at least two experts. draft reports written on each topic...
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Large chondroitinsulphate‐containing proteoglycan (versican) isolated from rabbit lung was cleaved by purified gelatinase A (MMP‐2) and B (MMP‐9), as well crude enzyme extract with hydraulic edema. Gelatine zymography, performed after purification of gelatinases affinity chromatography, demonstrated that the contained two main gelatinolytic bands at about 92 kDa 72 kDa, identified specific antisera latent proMMP‐9 proMMP‐2, respectively. Moreover, edematous showed an increased amount...
In anesthetized adult rabbits, pulmonary perivascular interstitial pressure (P(ip)), measured by micropuncture technique with intact pleural space, averaged -10.5 +/- 1.9 (SD) cmH2O in control conditions, a wet-to-dry lung weight ratio (W/D) of 4.8 0.2. Saline infusion (120 ml i.v. over 120 min) induced edema, increasing P(ip) to 3.62 1.6 no significant increase W/D (5.13 0.1). For intravenous saline exceeding 140 ml, decreased about atmospheric development severe edema that was...
1. During respiratory efforts against a closed airway, the afferent activity of vagal fibres from pulmonary stretch receptors does not appreciably increase during inspiratory phase because lung is prevented expanding. 2. Occlusion at different levels airways allows localization in tracheo‐bronchial tree. 3. 144 on left side tree have been studied eleven dogs and their was as follows: 17·4% upper half intrathoracic trachea, 27·1% lower trachea carina, 11·1% main bronchus, 13·9% lobe 30·5%...
Pulmonary interstitial pressure was measured via micropuncture in anesthetized rabbits normoxia and after breathing 12% O 2 . In [arterial Po = 88 ± (SD) mmHg], pulmonary arterial were 16 8 −9.6 cmH O, respectively. After 6 h of hypoxia (arterial 39 mmHg), the corresponding values 30 3.5 2.5 ( P < 0.05). proteoglycan extractability, evaluated by hexuronate assay 0.4 M guanidinium hydrochloride extraction, 12.3, 32.4, 60.6 μg/g wet tissue 3 hypoxia, respectively, indicating a weakening...
Pulmonary edema induces changes in airway and lung tissues mechanical properties that can be measured by low-frequency forced oscillation technique (FOT). It is preceded interstitial which characterized the accumulation of extravascular fluid space air-blood barrier. Our aim was to investigate impact early stages development on respiratory system. We studied 17 paralysed mechanically ventilated closed-chest rats (325–375 g). Total input system impedance (Zrs) derived from tracheal flow...
Objective: Recent ultrasonographic studies suggest that the administration of surfactant to preterm infants with respiratory distress syndrome (RDS) does not affect lung water clearance. The purpose study was also look at clearance in rabbits receiving surfactant.
We extracted and isolated proteoglycans from lung tissue samples obtained three groups of anesthetized rabbits: 1) control animals (C; n = 8) killed by overdose after 180 min; 2) receiving an intravenous saline infusion (S; 4, 1.5 ml . kg-1 min-1) for 3) bolus 200 microg pancreatic elastase (E; 4), min. The dry weight-to-wet weight ratio in the was 5.2 +/- 0.2, 6.0 0.4, 5.6 0.5, respectively. Gel-filtration analysis showed a massive fragmentation versican family extracellular matrix (ECM) S...
Mild pulmonary interstitial edema was shown to cause fragmentation of matrix proteoglycans. We therefore studied compartmental fluid accumulation by light and electron microscopy on lungs anesthetized rabbits fixed in situ vascular perfusion after 0.5 ml · kg −1 min iv saline infusion for 180 causing ∼6% increase lung weight. Morphometry showed that a relevant portion (44%) extravascular is detected early the alveolar septa, 85% this accumulating thick air-blood barrier. The arithmetic mean...
We studied responses of endothelial and epithelial cells in the thin portion air-blood barrier to a rise interstitial pressure caused by an increase extravascular water (interstitial edema) obtained anesthetized rabbits receiving saline infusion (0.5 ml.kg(-1).min(-1) for 3 h). morphometric analyses their microenvironment (electron microscopy); furthermore, we also lung tissue extracts biochemical alterations proteins responsible signal transduction (PKC, caveolin-1) cell-cell adhesion...
The volume-pressure relationship of the lung was studied in six subjects on changing gravity vector during parabolic flights and body posture. Lung recoil pressure decreased by ∼2.7 cmH 2 O going from 1 to 0 vertical acceleration (G z ), whereas it increased ∼3.5 30° tilted head-up supine postures. No substantial change found 1.8 G . Matching changes relationships chest wall (previous data), results a decrease functional respiratory capacity ∼580 ml at relative ∼1,200 Microgravity causes...
We evaluated the effect of pancreatic elastase (7 IU iv) on pulmonary interstitial pressure (P ip ) in situ rabbit lungs by a micropuncture technique through intact parietal pleura. P was −10.8 ± 2.2 (SD) cmH 2 O control condition, increased to +5.1 1.7 at ∼60 min [condition referred as mild edema (ME)], and subsequently decreased −0.15 0.8 O, remaining steady from 80 up 200 with marked increase lung wet-to-dry weight ratio severe (SE)], suggesting an tissue compliance. functionally...
A correlation between interstial pulmonary matrix disorganization and lung cellular response was recently documented in cardiogenic interstitial edema as changes the signal-cellular transduction platforms (lipid microdomains: caveoale lipid rafts). These findings led to hypothesize a specific "sensing" function by cells resulting from perturbation cell-matrix interaction. We reason that interaction may differ hypoxic type of due observed difference sequential degradation proteoglycans (PGs)...