A5037457228- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Fungal Biology and Applications
- Vestibular and auditory disorders
- Palliative Care and End-of-Life Issues
- Geriatric Care and Nursing Homes
- Neural dynamics and brain function
- Medicinal Plants and Bioactive Compounds
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- Hearing, Cochlea, Tinnitus, Genetics
- Chemical Synthesis and Analysis
- Polysaccharides and Plant Cell Walls
- Phytochemistry and Biological Activities
- Phytochemistry and Bioactivity Studies
- Mitochondrial Function and Pathology
- Patient Dignity and Privacy
- Exercise and Physiological Responses
- Historical Art and Architecture Studies
- Dementia and Cognitive Impairment Research
- Retinal Development and Disorders
- Cancer-related molecular mechanisms research
- Neurobiology and Insect Physiology Research
- Cancer Cells and Metastasis
- Neuroinflammation and Neurodegeneration Mechanisms
University of Pavia
2016-2025
Institute on Aging
2019
Jagiellonian University
2019
European Association for Palliative Care
2019
Vrije Universiteit Brussel
2019
Kyiv City Clinical Oncology Center
2019
Università Cattolica del Sacro Cuore
2016
Neuroscience Institute
2012
University of Turin
2012
Mylan (Switzerland)
2012
This study presents the first comprehensive proteomic profile of an Italian strain Schizophyllum commune, a highly heterogeneous white-rot fungal species with significant potential for industrial, nutraceutical, cosmeceutical, and clinical applications. Three protein extraction methods their impact on yield resulting composition have been compared. Results revealed that combination Tris–Cl urea increases total variety enzymatic related to pivotal pathways. Notably, over 2000 proteins were...
Neurons process information in a highly nonlinear manner, generating oscillations, bursting, and resonance, enhancing responsiveness at preferential frequencies. It has been proposed that slow repolarizing currents could be responsible for both oscillation/burst termination high-pass filtering causes resonance (Hutcheon Yarom, 2000). However, different mechanisms, including electrotonic effects (Mainen Sejinowski, 1996), the expression of resurgent (Raman Bean, 1997), network feedback, may...
Synaptic activity can induce persistent modifications in the way a neuron reacts to subsequent inputs by changing either synaptic efficacy or intrinsic excitability. After high-frequency stimulation, long-term potentiation (LTP) of is commonly observed at hippocampal synapses (Bliss and Collingridge, 1993), excitability has recently been reported cerebellar deep nuclear neurons (Aizenmann Linden, 2000). However, potential coexistence these two aspects plasticity remained unclear. In this...
1. Current‐clamp recordings were made in whole‐cell patch‐clamp configuration from ninety‐one granule cells parasagittal cerebellar slices obtained 21‐ to 31‐day‐old rats. Recordings performed at 30 degrees C. 2. Resting membrane potential was ‐58 +/‐ 6 mV (n = 43). The voltage response step current injection showed inward rectification consistent with increasing input resistance during depolarization. Over ‐35 7 14) repetitive firing little or no adaptation activated. Spike frequency...
D'Angelo, Egidio, Paola Rossi, Simona Armano, and Vanni Taglietti. Evidence for NMDA mGlu receptor-dependent long-term potentiation of mossy fiber–granule cell transmission in rat cerebellum. J. Neurophysiol. 81: 277–287, 1999. Long-term (LTP) is a form synaptic plasticity that can be revealed at numerous hippocampal neocortical synapses following high-frequency activation N-methyl-d-aspartate (NMDA) receptors. However, it was not known whether LTP could induced the relay This particularly...
Long-term potentiation (LTP) is a synaptic change supposed to provide the cellular basis for learning and memory in brain neuronal circuits. Although specific LTP expression mechanisms could be critical determine dynamics of repetitive neurotransmission, this important issue remained largely unexplored. In paper, we have performed whole cell patch-clamp recordings mossy fiber-granule acute rat cerebellar slices studied its computational implications with mathematical model. During LTP,...
During long-term potentiation (LTP) at mossy fibre-granule cell synapses in rat cerebellum synaptic transmission and granule intrinsic excitability are enhanced. Although it is clear that changes mediated postsynaptically, there as yet no direct evidence for the site mechanism of transmission. To approach this problem, evoked postsynaptic currents (EPSCs) miniature (mEPSCs) were recorded by patch-clamp cerebellar slices obtained from P17-P23 rats. LTP was induced theta-burst stimulation...
Calcium-binding proteins such as calretinin are abundantly expressed in distinctive patterns the CNS, but their physiological function remains poorly understood. Calretinin is cerebellar granule cells, which provide major excitatory input to Purkinje cells through parallel fibers. Calretinin-deficient mice exhibit dramatic alterations motor coordination and cell firing recorded vivo unknown mechanisms. In present study, we used patch-clamp recording techniques acute slice preparation...
Variations in intracellular calcium concentration ([Ca2+]i) provide a critical signal for synaptic plasticity. In accordance with Hebb's postulate (Hebb, 1949), an increase postsynaptic [Ca2+]i can induce bidirectional changes strength depending on activation of specific biochemical pathways (Bienenstock et al., 1982; Lisman, 1989; Stanton and Sejnowski, 1989). Despite its strategic location processing, spatiotemporal dynamics their relationship plasticity at the cerebellar mossy fiber...
Several forms of long-term synaptic plasticity [long-term potentiation (LTP) and depression (LTD)] have been reported in the cerebellar circuit <i>in vitro</i>, but their determination vivo</i> was still lacking most cases. Here we show that, urethane-anesthetized rat, appropriate patterns facial tactile stimulation as well intracerebellar electrical can induce LTP LTD local field potentials recorded from granular layer Crus-IIa. prevailed control conditions, whereas during application...
Frailty is a geriatric syndrome associated with both locomotor and cognitive decline, implicated in poor quality of life negative health outcomes. One central question surrounding frailty whether phenotypic the impairment during aging. Using spontaneous behavioral tests by studying dynamic change aging, we demonstrated that two form vulnerability, recognition memory develop parallel therefore, integration motoric evaluations are imperative. We developed an integrated index based on...
Brain aging is a crucial risk factor for several neurodegenerative disorders and dementia. The most affected cognitive function memory, worsening early during aging. Inflammation oxidative stress are known to have role in pathogenesis of impairments, link exists between aging/frailty immunosenescence/inflammaging. Based on anti-aging properties, medicinal mushrooms represent source develop medicines functional foods. In particular, Hericium erinaceus (He) displays actions ranging from...
D'Angelo, Egidio, Giovanna De Filippi, Paola Rossi, and Vanni Taglietti. Ionic mechanism of electroresponsiveness in cerebellar granule cells implicates the action a persistent sodium current. J. Neurophysiol. 80: 493–503, 1998. Although substantial knowledge has been accumulated on cell voltage-dependent currents, their role regulating remained speculative. In this paper, we have used patch-clamp recording techniques acute slice preparations to investigate ionic basis rat at mature...
In the cerebellum, granule cells are inhibited by Golgi through GABAergic synapses generating complex responses involving both phasic neurotransmitter release and establishment of ambient gamma-aminobutyric acid (GABA) levels. Although at this synapse mechanisms postsynaptic integration have been clarified to a considerable extent, remained largely unknown. Here we investigated quantal properties during repetitive neurotransmission, revealing that tonic GABA(B) receptor activation GABA...
Although the role of abnormal prion protein (PrP) conformation in generating infectious brain diseases (transmissible spongiform encephalopathy) has been recognized, function PrP normal remains mostly unknown. In this investigation, we considered effect gene knock-out (PrP 0/0 ) on cerebellar neural circuits and particular granule cells, which show intense expression during development selective affinity for PrP. At third postnatal week, when would normally attain mature levels, mice showed...
ObjectivesAlthough a growing number of older people are dying in care homes, palliative has developed these settings only recently. Cross-country representative comparative research hardly exists this area. As part large EU-funded project, we aim to undertake homes Europe, describe and compare 6 countries terms (1) resident outcomes, quality costs end-of-life care; (2) structures staff knowledge attitudes toward care. We also explore country, facility, staff, patient, characteristics related...